FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2E5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2E5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5188
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT139726.92752505782575No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT119022.937548188126446No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA92717.86815728604472No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA84216.22976098689283No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA500.963762528912876No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT470.9059367771781034No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA330.6360832690824981No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT260.5011565150346955No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA240.4626060138781804No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT220.4240555127216654No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA200.3855050115651504No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT140.2698535080956052No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA140.2698535080956052No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA130.25057825751734775No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT120.2313030069390902No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTTA110.2120277563608327No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGTCA100.1927525057825752No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT100.1927525057825752No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT80.15420200462606012No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT70.1349267540478026No Hit
GACTACAACAGGAGTAGAGACCCGGGTAGGCCACGCCGTAAACGAGGAGT70.1349267540478026No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGA60.1156515034695451No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA60.1156515034695451No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGTCA60.1156515034695451No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA60.1156515034695451No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGTAA60.1156515034695451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)