FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2E4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2E4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4540
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT150533.14977973568282No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT123227.13656387665198No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA53211.71806167400881No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA45510.022026431718063No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA581.277533039647577No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT491.079295154185022No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA430.9471365638766519No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT320.7048458149779736No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA220.4845814977973568No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT190.4185022026431718No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT120.2643171806167401No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA120.2643171806167401No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT110.2422907488986784No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA100.22026431718061676No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTTA90.19823788546255508No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT80.1762114537444934No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT80.1762114537444934No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT80.1762114537444934No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT80.1762114537444934No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA80.1762114537444934No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT70.15418502202643172No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGA70.15418502202643172No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA60.13215859030837004No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA60.13215859030837004No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT60.13215859030837004No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT60.13215859030837004No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAAAGATGAGT50.11013215859030838No Hit
GACTACACCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT50.11013215859030838No Hit
GACTACAACAGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT50.11013215859030838No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTTA50.11013215859030838No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGCGT50.11013215859030838No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGTCA50.11013215859030838No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT50.11013215859030838No Hit
GACTAAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG50.11013215859030838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)