FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2E1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2E1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA6753.6No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA4233.6No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT21.6No Hit
GACTACAACCGTTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAAC21.6No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCT10.8No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCG10.8No Hit
GACTACAACAGGATTAGCTACCCTGGTAGTCCACGCCCTAAACGATGTCA10.8No Hit
GAACTACAACCGGATTAGATACCCTGGTAGTCCACGACGTAAACGATGAG10.8No Hit
AACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA10.8No Hit
GAACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAG10.8No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA10.8No Hit
GACTACAACCGGATTAGATACCCTGGTAGTTCACGCCCTAAACGATGTCA10.8No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTACACGATGTCA10.8No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTCAACGATGTCA10.8No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA10.8No Hit
GACTACAACAGGATTAGATACCCTGGTATTCCACGCCCTAAACGATATCA10.8No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)