FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7422
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT309341.67340339531124No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT261035.16572352465643No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA4646.251684182161142No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA4526.090002694691457No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT901.2126111560226354No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT881.1856642414443546No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT300.40420371867421184No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG270.3637833468067906No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT150.20210185933710592No Hit
GACTCAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG140.18862840204796552No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT110.14820803018054432No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG100.13473457289140395No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG90.12126111560226355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)