FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5367
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT233443.48798211291224No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT195236.370411775666106No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA991.8446059250978202No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT861.6023849450344698No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA711.3228991988075276No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA611.1365753679895658No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA601.1179429849077696No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT510.9502515371716043No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT370.689398174026458No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT310.577603875535681No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT220.40991242779951553No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT200.3726476616359232No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT170.31675051239053476No Hit
GACTCAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG130.2422209800633501No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG120.22358859698155395No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG110.20495621389975777No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT100.1863238308179616No Hit
GACTACAACAGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT100.1863238308179616No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGAGT90.16769144773616546No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT80.1490590646543693No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGCGA80.1490590646543693No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT80.1490590646543693No Hit
GACTACAACGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG80.1490590646543693No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT70.13042668157257314No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT60.11179429849077697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)