FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2B5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2B5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6068
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT255742.139090309822016No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT207234.146341463414636No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA3295.42188529993408No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2834.66381015161503No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT560.922874093605801No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT520.8569545154911009No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT210.34607778510217535No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG180.2966381015161503No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT150.24719841793012526No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT140.23071852340145024No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA140.23071852340145024No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT120.1977587343441002No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTGT120.1977587343441002No Hit
GACTCAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG120.1977587343441002No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT110.1812788398154252No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT110.1812788398154252No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA100.16479894528675015No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT90.14831905075807514No Hit
GACTACAACAGGAGTAGAGACCCGGGTAGGCCACGCCGTAAACGAGGAGT80.13183915622940012No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG80.13183915622940012No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT80.13183915622940012No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT70.11535926170072512No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATAAGT70.11535926170072512No Hit
GGACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAG70.11535926170072512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)