FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2A4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2A4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6038
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT232938.57237495859556No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT217135.955614441868164No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA4307.121563431599867No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA3365.564756541901292No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT691.142762504140444No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT470.7784034448492879No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT190.31467373302418017No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT140.23186485591255385No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT130.21530308049022856No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA130.21530308049022856No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA110.18217952964557801No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT100.16561775422325273No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG100.16561775422325273No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT90.14905597880092747No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTGT90.14905597880092747No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT80.13249420337860218No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTGT80.13249420337860218No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT80.13249420337860218No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT80.13249420337860218No Hit
GACTACAACCGGATTAGATACCCTGGTATTCCACGCCGTAAACGATGAGT70.11593242795627692No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGT70.11593242795627692No Hit
GACTCAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG70.11593242795627692No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG70.11593242795627692No Hit
GACTACAACCGGATTGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG70.11593242795627692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)