FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP2A1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP2A1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length301
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT2227.848101265822784No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT2025.31645569620253No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA1215.18987341772152No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA78.860759493670885No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA78.860759493670885No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT67.59493670886076No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATTTCA11.2658227848101267No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGTGT11.2658227848101267No Hit
GACTACAACCGGATTAGATACCCTGATAGTCCACGCCGTAAACGATGAGT11.2658227848101267No Hit
GACTACAACAGGATTAGATACCCTGATAGTACACGCCGTAAACGATGAGT11.2658227848101267No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)