FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP1H4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP1H4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5554
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT211338.04465250270076No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT188934.01152322650342No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA3806.841915736406194No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA2975.347497299243789No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT631.1343176089305007No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT571.026287360460929No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT260.4681310767014764No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT250.45012603528988115No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA190.34209578682030967No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA190.34209578682030967No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT160.28808066258552395No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT140.25207057976233344No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT130.2340655383507382No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT120.21606049693914295No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG110.19805545552754772No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGC110.19805545552754772No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT100.18005041411595246No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA100.18005041411595246No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT90.16204537270435723No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA80.14404033129276198No Hit
GACTCAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG70.12603528988116672No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT70.12603528988116672No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG70.12603528988116672No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATAAGT60.10803024846957147No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT60.10803024846957147No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT60.10803024846957147No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGAGT60.10803024846957147No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA60.10803024846957147No Hit
GACTACAACAGGAGTAGAGACCCGGGTAGGCCACGCCGTAAACGAGGAGT60.10803024846957147No Hit
GACTACACCAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT60.10803024846957147No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGAGT60.10803024846957147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)