FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP1G8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP1G8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52522
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT2158241.09135219527055No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT1857835.37184417958189No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA13332.5379840828605156No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA11762.2390617265146036No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA7481.42416511176269No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA6851.3042153764136932No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT5491.0452762651841134No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT5301.0091009481740985No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT3760.7158904839876623No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT3130.5959407486386658No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT1560.2970183922927535No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT1370.26084307528273865No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT1250.23799550664483454No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT1230.2341875785385172No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG1000.19039640531586766No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT870.16564487262480485No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG870.16564487262480485No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT780.14850919614637675No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT730.13898937588058338No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT720.1370854118274247No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTGT700.13327748372110734No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT680.12946955561478998No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG620.11804577129583795No Hit
GACTACAAACGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT600.11423784318952059No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGAGT590.11233387913636192No Hit
GACTACAACCGGATTAGATACACTGGTAGTCCACGCCGTAAACGATGAGT580.11042991508320321No Hit
GACTACAACAGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT570.10852595103004455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)