FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP1F8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP1F8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35981
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA1558743.32008560073372No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA1336137.13348711820127No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA4461.2395430921875434No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA3881.0783469053111365No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTAA1840.5113810066423946No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTACACGATGTCA1690.4696923376226341No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTAA1650.45857535921736475No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTACACGATGTCA1520.42244517940023907No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA1510.41966593479892167No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCACTAAACGATGTCA1260.3501848197659876No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTTA1180.32795086295544873No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA1170.3251716183541314No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGAGGTAA1070.29737917234095773No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGAGGTAA1070.29737917234095773No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCACTAAACGATGTCA1000.2779244601317362No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTTA900.25013201411856256No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA700.19454712209221534No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT670.18620938828826325No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCATAAACGATGTCA650.18065089908562854No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCGCTAAACGATGTCA640.17787165448431116No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCATAAACGATGTCA640.17787165448431116No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT580.161196186876407No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCGCTAAACGATGTCA580.161196186876407No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCCTAAACGATGTCA500.1389622300658681No Hit
GACTACAACAGGATTAGCTACCCTGGTAGTCCACGCCCTAAACGATGTCA500.1389622300658681No Hit
GACTACACCAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA460.12784525166059865No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT450.12506600705928128No Hit
GACTACAACAGGATTAGATACCATGGTAGTCCACGCCCTAAACGATGTCA440.12228676245796392No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTGA410.11394902865401184No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCCTAAACGATGTCA410.11394902865401184No Hit
GACTACAAACGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA390.10839053945137711No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCCTAAACGAGGTAA370.1028320502487424No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCT370.1028320502487424No Hit
GACTCAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAA360.10005280564742502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)