FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP1E1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP1E1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6626
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT230434.772109870208276No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT200430.244491397524904No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA5678.557198913371566No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA5338.044068819800785No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA1512.2789012979172956No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA1071.6148505885904014No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT811.2224569876245095No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT580.8753395713854513No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT560.8451554482342287No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT530.7998792635073951No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA190.2867491699366133No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA180.27165710836100215No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT150.22638092363416845No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA140.21128886205855718No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT130.196196800482946No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA130.196196800482946No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGCGA130.196196800482946No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGAGT110.1660126773317235No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGC110.1660126773317235No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT110.1660126773317235No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA100.1509206157561123No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT100.1509206157561123No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTGT90.13582855418050108No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT90.13582855418050108No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG90.13582855418050108No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA90.13582855418050108No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT80.12073649260488982No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA70.10564443102927859No Hit
GACTACAAACGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT70.10564443102927859No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTCAACGATGAGT70.10564443102927859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)