FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP1B9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP1B9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61432
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT2147434.95572340148457No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT1873130.49062377913791No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA42676.945891392108347No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA35915.845487693710118No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA20023.258887876025524No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA16802.734731084776664No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT5390.8773928896991795No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT4440.7227503581195468No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT3950.6429873681468942No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT3890.6332204714155489No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGCGA2120.3450970178408647No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGCGA1560.2539393150149759No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT1510.24580023440552157No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA1390.22626644094283108No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT1390.22626644094283108No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA1290.2099882797239224No Hit
GACTACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG1230.20022138299257713No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT1160.18882667013934107No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT1080.17580414116421408No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA1060.17254850892043236No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT920.14975908321396017No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG860.1399921864826149No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT820.13348092199505143No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA730.1188305768980336No Hit
GACTCAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG710.11557494465425186No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA690.1123193124104701No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT620.10092459955723403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)