FastQCFastQC Report
Mon 17 Apr 2023
000000000-KWF5G_l01_n01_ELP1A4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KWF5G_l01_n01_ELP1A4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24841
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT770631.02129543899199No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT685327.58745622156918No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA21698.731532546998913No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA18927.616440561974155No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA10064.049756451028542No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA9353.7639386498128093No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT2250.9057606376554889No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT2120.8534278008131718No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAAT2040.8212229781409767No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT1550.6239684392737812No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGC880.3542530493941468No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA860.34620184372609797No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA790.31802262388792724No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGC730.29386900688378087No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATA710.2858178012157321No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA620.24958737570951248No Hit
GACTACAACAGGATNAGATACCCTGGTAGTCCACGCCGTAAACGATGTCA580.23348496437341493No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT580.23348496437341493No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTACACGATGAGT560.22543375870536614No Hit
GACTACACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTG440.1771265246970734No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT410.1650497161950002No Hit
GACTACAACAGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT400.1610241133609758No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGA390.1569985105269514No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGAGGAGT380.152972907692927No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA370.14894730485890262No Hit
GACTACAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGCGA370.14894730485890262No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT350.14089609919085383No Hit
GACTACAACCGGAGTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGT350.14089609919085383No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGCGA340.13687049635682944No Hit
GACTACAACCGGATNAGATACCCTGGTAGTCCACGCCCTAAACGATGTCA300.12076808502073186No Hit
GACTACAACAGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT290.11674248218670746No Hit
GACTACAACCGGATTAGCTACCCTGGTAGTCCACGCCGTAAACGATGAGT260.10466567368463428No Hit
GACTACAACCGGATTAGATACCATGGTAGTCCACGCCGTAAACGATGAGT250.10064007085060989No Hit
GACTACAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTAA250.10064007085060989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)