FastQCFastQC Report
Fri 18 Nov 2022
000000000-KN4F5_l01_n01_S3-2022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KN4F5_l01_n01_S3-2022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179230
Sequences flagged as poor quality0
Sequence length251
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC111226.205434358087373TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC12820.7152820398370808TruSeq Adapter, Index 5 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2370.13223232717736985TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAGCTCGTATGC2050.1143781732968811TruSeq Adapter, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)