FastQCFastQC Report
Sat 4 Feb 2023
000000000-KMC2R_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KMC2R_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23828745
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGACAAGTTTGTCTTCGCGTCTG8083023.3921299673986187No Hit
TCTTTCCCTACACGACGCTCTTCC7654383.212246385615357Illumina Single End PCR Primer 1 (100% over 24bp)
GGTGATTAGTTAGCTTCGCGTCTG7396183.1038898607543115No Hit
TGCGCGTGTGGACATACGCGTCTG6158822.584617863844697No Hit
TTAGCGCTCCCGCTGACGCGTCTG5036612.113669855462384No Hit
CGTAATAAGGTAAGTCCGCGTCTG3349271.40555870651182No Hit
CCAACCTAGTCTTAGTCGCGTCTG2860621.2004912554144165No Hit
TAAAGCATCTTTGTGGCGCGTCTG2860481.2004325028447784No Hit
AAGTGGCGTACTAACCCGCGTCTG2747191.1528890841712394No Hit
AGGGTTTAGCCAATCCCGCGTCTG2658381.1156189719601262No Hit
CAAAGGCGTAACCGGGCGCGTCTG2353760.9877817736519485No Hit
GTTACCGGTAGGCGCACGCGTCTG1826120.7663517319103461No Hit
GCCGTTAGTGCAAGTACGCGTCTG1805450.7576773346644987No Hit
ATAATGGTCGAAGTTACGCGTCTG1639090.6878624954860191No Hit
CGTTCCTGTGCAACAACGCGTCTG1621750.680585570075134No Hit
CCGTTCCTCACTTGCACGCGTCTG1505320.6317244151968557No Hit
CGCAACAAGATTAGGCCGCGTCTG1465550.6150344888075305No Hit
GAATTAGTCGATAAGCCGCGTCTG1441840.605084321478114No Hit
CATGAGGTCGTTAGTTCGCGTCTG1394300.5851336274738766No Hit
AGAGCCGGTTTCTCATCGCGTCTG1389480.5831108604334807No Hit
CTAGTAAAGTTATGGACGCGTCTG1360530.5709616683547538No Hit
GGTTACCTCACACAAACGCGTCTG1344050.5640456515859312No Hit
TTTAAGCTCATTCAGTCGCGTCTG1335950.5606463957711579No Hit
TAGTGTGCAGCCAATACGCGTCTG1329080.5577633232467761No Hit
TCACGCCCACTCAGTTCGCGTCTG1183640.496727796617069No Hit
ATAGGTTCAACAGAGGCGCGTCTG1156900.48550605581620015No Hit
TCCTTGCGTCGGTTATCGCGTCTG1087090.4562095066273948No Hit
CCCAGCTCACCTCGACCGCGTCTG1078650.45266756600064334No Hit
AAATCGATCTCCATGACGCGTCTG1021140.428532849715753No Hit
TGTTCCCGTCGAGGCACGCGTCTG1015330.4260946180757736No Hit
GGCTATGAGCTATCGCCGCGTCTG1009020.423446555829944No Hit
GCATGCAAGGCTAGCACGCGTCTG984140.4130053848828379No Hit
GGCGATTCAGCTCGAGCGCGTCTG979900.41122602134522823No Hit
GACTTTGTCTTCAAGTCGCGTCTG968320.40636634451373754No Hit
CGTGCCTCATCACAGACGCGTCTG967250.40591730701721807No Hit
GCAAGAAAGAGCAAGACGCGTCTG943020.3957489158577172No Hit
GAGTGATTCCGCACATCGCGTCTG934140.39202232429781764No Hit
GACCAGGGTCGGTTCACGCGTCTG926480.3888077194161925No Hit
TAGTGTGCAACAAAGACGCGTCTG925270.388299929350035No Hit
GCTCCTGAGGAGCTTGCGCGTCTG900120.3777454498757698No Hit
GGGCCATAGCGCAAATCGCGTCTG897380.3765955781557107No Hit
GGAGCATGTACCTTGGCGCGTCTG853970.35837808495579604No Hit
GTCCTAATCTCAGCTACGCGTCTG838150.35173904458669564No Hit
GGCAAGCTCGCTTGACCGCGTCTG824570.3460400453318041No Hit
GTTCCAGGTAGGTTAGCGCGTCTG804700.337701377055317No Hit
ATCGCCTAGCAATTGACGCGTCTG767340.3220228341861898No Hit
TCCTTAGAGGTCCCTGCGCGTCTG764790.3209526980963538No Hit
CATCCGTAGTCCTCCCCGCGTCTG736120.3089210111569032No Hit
TCTTTCCCTACACGACGCCCTTCC719920.3021224995273566Illumina Single End PCR Primer 1 (95% over 24bp)
AGTAGGCGTTTCCAGGCGCGTCTG694590.29149248103498526No Hit
GAGTCAAGTCAACCGCCGCGTCTG687290.2884289541895723No Hit
TCAAGTGCACTCAGTTCGCGTCTG678550.28476111519931074No Hit
GGTTGCAAGATAACTGCGCGTCTG669110.2807995133608589No Hit
CCAACTGGTAAGTGCACGCGTCTG663660.27851235975709165No Hit
ACCTCCCTCGCTCAACCGCGTCTG615080.25812521809268596No Hit
ATATTGCTCCTTATGGCGCGTCTG561910.23581183146657533No Hit
GCTCAAACAGGATAAACGCGTCTG539210.22628552196097612No Hit
GCACCTTAGTGCTCACCGCGTCTG520290.21834553183560443No Hit
TGAGGTCCATAGCATGCGCGTCTG509770.21393069588851615No Hit
AAGTGGCGTATCCGTGCGCGTCTG497180.20864716123320803No Hit
GCCATTATCCTTGCAACGCGTCTG478220.20069038465936836No Hit
GTCATAAAGCAATAGCCGCGTCTG477840.20053091339892218No Hit
GGCTTGCGTTGACTACCGCGTCTG473280.19861725827356835No Hit
GCCTATATCGCTTGCGCGCGTCTG453830.19045484770599544No Hit
TAAAGCATCTTGCAGCCGCGTCTG428270.17972830713493304No Hit
ATTGGCGAGGACTTAACGCGTCTG427490.17940097138980673No Hit
ATGGCCTTCCTAATGTCGCGTCTG425690.178645581208746No Hit
GAGGTTAGTTGCTCTCCGCGTCTG411450.1726696055541322No Hit
GGAGGATAGGCGATATCGCGTCTG405370.17011806538699376No Hit
ACAACGGTCCGAAACGCGCGTCTG402150.16876675628531843No Hit
CTTATGAGTTGTCATACGCGTCTG389340.16339089616343622No Hit
GGTTGTTGTCAATTGCCGCGTCTG381020.15989931488208883No Hit
TGTGCGGCAGTACCGGCGCGTCTG379510.15926562645242123No Hit
AGGGCCTAGCCAGTAGCGCGTCTG377620.15847246676230745No Hit
TGCAGGTCAAGCTCAACGCGTCTG376040.1578094020478208No Hit
CAAGGTGTCGGTTACCCGCGTCTG367580.15425906819683538No Hit
ATTAGGATCGCGAGTACGCGTCTG367520.15423388852413333No Hit
GGCTAAGTCATTCAGTCGCGTCTG366620.153856193433603No Hit
GCCACTTAGATTCATCCGCGTCTG365090.15321411177970135No Hit
GCATTATTCACTAAGACGCGTCTG363950.1527356979983629No Hit
CAATTGCGTTACAACCCGCGTCTG360590.15132563632704954No Hit
TCGATTAGTCTATCTTCGCGTCTG360440.15126268714529448No Hit
GTTCTGTCATAATGCGCGCGTCTG359420.15083463270936004No Hit
TTGCTGGGTTCAAGCTCGCGTCTG358560.1504737240672977No Hit
GCGGTAGGTTTCCAGGCGCGTCTG353120.14819076707564752No Hit
CTTCCTCCACCGTTCACGCGTCTG349570.14670096977411107No Hit
ATGGCTTGTCACACCACGCGTCTG348270.14615541019890052No Hit
ATTGTCAGTTTCCGGACGCGTCTG346180.14527831826644666No Hit
AGTTTCTAGCTCAAGGCGCGTCTG339730.14257150345097905No Hit
TAACCACGTTTCTAAGCGCGTCTG339250.14237006606936287No Hit
GGGTTGGGTACAATGGCGCGTCTG332850.13968423431448027No Hit
TACAACAAGCCTTCAACGCGTCTG326270.13692286354149158No Hit
GGAAATTGTACCTTGGCGCGTCTG314810.13211354605540493No Hit
TAGTAGCAGCTAACTTCGCGTCTG311800.1308503658081867No Hit
GGTCGAGGTTTGTAGCCGCGTCTG304470.12777424912642274No Hit
GGTTTACGTTCCATTTCGCGTCTG304190.12765674398714663No Hit
AGCATCCTCAAGGTCGCGCGTCTG303610.12741334048436037No Hit
CCGGTTAGTTACGAAGCGCGTCTG302370.1268929605818519No Hit
CGTGATGAGGGAGTAACGCGTCTG302360.12688876396973486No Hit
AATTGAGTCAAGCTTTCGCGTCTG289540.12150870723573566No Hit
CCTTCTAGTCACACCACGCGTCTG288110.12090859170300408No Hit
ACGTTTCCATTAACCCCGCGTCTG285390.11976711320717896No Hit
CTTACTTGTCCTGAGCCGCGTCTG283100.11880608903238506No Hit
TCATTGAGTAGCTAAGCGCGTCTG278450.11685466439797815No Hit
TTGGTCCAGCTTTACGCGCGTCTG277720.11654831171343685No Hit
GTTAGAGGTGCAACGGCGCGTCTG277370.11640143028934173No Hit
GGAGTAGCAAGGATGGCGCGTCTG276320.11596078601705628No Hit
CGTCAATGTCTTGACGCGCGTCTG265540.11143683815492592No Hit
GACAAACTCCGTCCAGCGCGTCTG259400.10886011831508541No Hit
GTTGGTGAGAAGCTACCGCGTCTG259020.10870064705463926No Hit
ATAGCTAAGTCACTATCGCGTCTG256950.10783194834641942No Hit
TAGCAAGGTTATGGGACGCGTCTG255160.1070807547774757No Hit
ACAGCCTTCCAATTACCGCGTCTG255060.10703878865630566No Hit
AGTAACGGTTCACTTTCGCGTCTG254670.10687512078374249No Hit
TAAAGGGAGCCTTAATCGCGTCTG253840.10652680197803116No Hit
CTTATGAGTTCTTAAGCGCGTCTG252440.10593927628165059No Hit
GAACTTAAGGCAACATCGCGTCTG252270.10586793387566151No Hit
TTCGTTACAATCTCTACGCGTCTG251560.10556997441535423No Hit
CTAGCCTAGCTTGCTACGCGTCTG248100.10411794662287081No Hit
AACGTACAGGGTTATACGCGTCTG245640.10308558004208783No Hit
TGCTGGAAGTTTGAGACGCGTCTG244390.10256100352746232No Hit
AAAGGCAAGCAAGACTCGCGTCTG244340.1025400204668773No Hit
CAAATCAAGGCCAACACGCGTCTG242760.10187695575239064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)