FastQCFastQC Report
Sat 4 Feb 2023
000000000-KMC2R_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KMC2R_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23828745
Sequences flagged as poor quality0
Sequence length8
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT623990526.18646093195424No Hit
TAGGTAAA567584723.819328294461165No Hit
ACCAGTCC536335922.507937367242796No Hit
GGACAGGG452146018.974813822549194No Hit
ACAGTGAT1837770.7712407850266558Illumina PCR Primer Index 5 (100% over 8bp)
ACCAGTCA640260.2686922874033022No Hit
CTTCCTTA447980.18799982961754805No Hit
CTTTCCTA445760.18706818172757314No Hit
ACAGTCCA346670.14548395226017988No Hit
GGACAGGA334180.1402423837260418No Hit
CTTTCTTA295300.12392595581513001No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)