FastQCFastQC Report
Sat 4 Feb 2023
000000000-KMC2R_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KMC2R_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23828745
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTTCCTTATCTCGTATGCCGT1495350.6275403929162027No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGTAAAATCTCGTATGCCGT1278800.5366627575224797No Hit
CTTATACACATCTCCGAGCCCACGAGACACCAGTCCATCTCGTATGCCGT1121570.47067942520682476RNA PCR Primer, Index 16 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACAGGGATCTCGTATGCCGT755030.31685680467015787No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTTCCTTATCTCGTATGCCG511280.21456438431818378No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGGTAAAATCTCGTATGCCG445600.18700103593370107No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCAGTCCATCTCGTATGCCG393400.16509472068293987No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTTCCTTATCTCGTATGCC293030.12297332486457008No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACAGGGATCTCGTATGCCG261940.10992605779280445No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGTAAAATCTCGTATGCC258400.10844045710338501No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA251420.10551122184571617No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA244960.10280021041813156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)