FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n02_nHRB12LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n02_nHRB12LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGTCCGTGTAG80.3683241252302026Illumina Single End PCR Primer 1 (97% over 35bp)
GAACTCCAGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGTC40.1841620626151013Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGTCCGTGTAGATCT40.1841620626151013Illumina Single End PCR Primer 1 (97% over 35bp)
CCAGTCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGTCCGTG40.1841620626151013Illumina Single End PCR Primer 1 (97% over 35bp)
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGTCCGT40.1841620626151013Illumina Single End PCR Primer 1 (97% over 35bp)
GTGACTGGAGTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCG40.1841620626151013Illumina Single End PCR Primer 1 (97% over 35bp)
GAACTCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGTCCG30.13812154696132595Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)