FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n02_HRB0342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n02_HRB0342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAGAGTAAATTGCGTTGACGGTACAAGAACTTGGCGGGTGGGTGCGAT10.9615384615384616No Hit
GAATGGAACAAAAAGGTTACAGGCTTTAGGAGGAAGATTGTAAGGTGAGC10.9615384615384616No Hit
CGCTATCAAAACTCTCGATCATGCAATGTCAGGGCCAGCTGGATAGATCG10.9615384615384616No Hit
CAACTGCACCGCGCTGCAGTACGTCGAAGATCGGAAGAGCGTCGTGTAGG10.9615384615384616Illumina Single End PCR Primer 1 (100% over 23bp)
GGACACCACGTCAGGGAAACCACTCTCCAAGAACATCGAGGGAGTCAAAA10.9615384615384616No Hit
CACCTCTGTCTCCTGCACCGACTCCTCCTCGGGCTCCTGCACCGACTCCT10.9615384615384616No Hit
TATTGGAGTGTATTGGATGCGTTCGTGGCAAAAACTGACTTCATGATTCG10.9615384615384616No Hit
ATGTTACTTGGCGTATAATAATCTCTATATGTCTATTATGGATCTGTACC10.9615384615384616No Hit
TGCCACGGCCGCTGACCTTGATAACTTTTTTATTTGAAATCTTTTAGATA10.9615384615384616No Hit
TTGTGCGCGCGGAAAACGAGGGAAAGAGGTTGGGAACATCGGGTGACTGG10.9615384615384616No Hit
TTACAACTTCTCCACCAACATTCTCTTCTCAACCAATCACAAAGATCGGA10.9615384615384616No Hit
ATATGTACAAAAATCATGTTTTGGTGCTTTAGATCGGAAGAGCGACGAGT10.9615384615384616No Hit
CCATTCGAACCGCCGAGATCACCTGTCGCTGACCGCAGATCGGAAGAGCG10.9615384615384616No Hit
AAATCGGCCGACCGACGAAAGATGGTTTCTCCACCAGTGAATCTTGTCTT10.9615384615384616No Hit
CTGGCTTGGTTCCACTGGCGCCGACTGGTGTTTTTGTCACCATCGATGGA10.9615384615384616No Hit
AATATTATAAATTTAAAAAATAGATTTGTTCGATATTTCAAAGCAACAGA10.9615384615384616No Hit
TTTTTGACCGGAAAGAAAATGATCAAAAAGCATAGTAGTACTCCAGTACC10.9615384615384616No Hit
GTGGTTCTCCCAGGCAAAAGAAAAATCGTCGGAGTTGAGGACAAGACTGA10.9615384615384616No Hit
CTCTGATTAACTTTCTATTGCACTCAAGATCCTGTGGCGCATGTACTGAT10.9615384615384616No Hit
GTTTTTGAGCAACTCCACAGGATCACTCGCTCCCGCAGCAACATCACTCG10.9615384615384616No Hit
TCCGACATGCTTGTATTAGAAAACTTCAACCACCAATTATCAGCAATAAG10.9615384615384616No Hit
TCCCTAGTCAACTATGACAAATGCTTAGAGATGCTACAGATCGGAAGAGC10.9615384615384616No Hit
TTGGTTTCTTTTCATTCAGGGTGCGTGCCGCTAGCGACAGACGAGATCGG10.9615384615384616No Hit
TTGATTTCCAATAAGGGTTAACCCACATGCCTTCCTCGCCATCAGATCGG10.9615384615384616No Hit
GTTCCAAATCAATTTTTGGCCATGTACACATAAAATTTGCTTTTCCATAA10.9615384615384616No Hit
TGCACCGCTGCGGCGAATACAACCATACAAGCGCGCGAATACAACTATAA10.9615384615384616No Hit
CGTGCCCACTTTCTCCATCCGAACTATCCATGACAACCATAGTTGTGTGG10.9615384615384616No Hit
CGTGTCTGTAGTCTGAATTAACCCGCACTCATAGTAAGATCGGAAGAGCG10.9615384615384616No Hit
AGCTTACACTGCCGCCCATCCTATCTCCAAACTCTCGCATCCGCAACAGC10.9615384615384616No Hit
GAAAGAACTTTCATCGCAACATCTCGCCCATTTCTAATAGTTCCCTGAAA10.9615384615384616No Hit
TGGATTTGCTGCGGCGCGCGGGTTGATATGAGGGGTGTCCCCTACAGATC10.9615384615384616No Hit
CCCCGGGGTGGGGGGCCAATTATCATGGCCTAGTGCTTAAACATGCAAGC10.9615384615384616No Hit
GAAACACCATATTCATCTAGCATAGTCCTTGCCATCTCAATCAATGTACG10.9615384615384616No Hit
GAGTAACTGTCACCCTATTTTTTAAAATATTTAATTTATCCCTAGACCAC10.9615384615384616No Hit
GATCGTGAGTTACTCATGTTACAGGCATTGGGGATGAACGAGGTATAGAT10.9615384615384616No Hit
TGTCCATCACGGCGATGATTTGTGATGATGCACTGCATCTGCATGCGACG10.9615384615384616No Hit
TTACGGCAATACATCGACGGTGACGGTTGACAGATCGGAAGAGCGTCGTG10.9615384615384616No Hit
AATTCTCCTCCATTGTCACTTCGTACCCTCTTGATGGTGAGATCATAAAG10.9615384615384616No Hit
CGAACAAGCTAGCTAGAGATCAAGCAACGCAGGCATGTCGTCGAGATCGG10.9615384615384616No Hit
AAATTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTAATTTTTTTT10.9615384615384616No Hit
TTTCTTCGATTTGCTTGCAGGAATAGGGTTGATTTGCACCGGCAAGATTA10.9615384615384616No Hit
ACACTTGAGGACACTGCAGGCCGTTATCTGACAGAAAGATCGGAAGAGCG10.9615384615384616No Hit
ATCCTCAACTCCTCTAAACAGTCCAAATTCCCGTCTATATTATGAGAATA10.9615384615384616No Hit
AGGTGTGGACCTGCAAAAAAATGAAAATCAACATAAATATGAGATCGGAA10.9615384615384616No Hit
GTATGTAAAGTTGGGGGTTGAAGGAGATGTCCGGTTTTGTGGTTCTGGGA10.9615384615384616No Hit
TCGAATCACAGGAACTAAGCCAACCAGAGCGGGAGATCGGAAGAGCCTCG10.9615384615384616No Hit
AGCAAAAGGAGGGAGGAGTGCAACAAAAGCAACAGAACAGAATCATATAT10.9615384615384616No Hit
TTTTAGTTTTTTTTCCGGTGATGAAAAGTTGTCATTTGAATGTGTACCAG10.9615384615384616No Hit
CATGTCGAGGCCGGCTTGGTTGGCGAAGGTGAACACAGGAGAGATCGGAA10.9615384615384616No Hit
TCCCTCTGCTCCTCTCCCCTCTTGTCCCTCGCGATGGCGAGATCGGAAGA10.9615384615384616No Hit
TAAAATGCAGCAAGTCCTCTGAAAAGACTTGTTAGTTGTTACTTGTTAGA10.9615384615384616No Hit
AAACCCAAATTGACGGAGCGATGGAGATGGAGATGGAGAGATCGGAAGAG10.9615384615384616No Hit
GCACTATGCTATCCTCTTTCATCCAACTTGAATGTATTTAAGATTAGAAG10.9615384615384616No Hit
CGTAGTGCAGAGCAACCACGATCTAACACCAAGGAGGCGTAGTTAGACTG10.9615384615384616No Hit
ACAAATTCAAATTTGAACATGCCTTAGTTGAATTCACAGAGATCGGAAGA10.9615384615384616No Hit
GGACAAAATCTGTCTCTGAAATGGGATCTGTTTGAATCGGATTCGATACG10.9615384615384616No Hit
CATGCTTAGGTTGAACTAAAGAACTGTGCTCCAATCCTCATCCAAGATCG10.9615384615384616No Hit
GCTACCCTTTTTATATGCAAGCAAAGCAGATCCTCGCAAAGCTTCTGTCA10.9615384615384616No Hit
AGAGCGACAGCGACAGGAGCGAGAACGACTACAGCGTGAGCAACAAGATC10.9615384615384616No Hit
CGGGCACTAGACAACAAGCAAAGAAACAAATTTCGCAATTAAGGTTTTAG10.9615384615384616No Hit
CCTAACCGACTGCTTTTTGGTAGCAGAAGTTAGCCGATAGATCGGAAGAG10.9615384615384616No Hit
TGTAATTGTAAAACCTTGGTTTGTTTATTAGTTTAATATATGTTCAGATC10.9615384615384616No Hit
ACATCTCCTGGAACGGAGCAGGCGACAGGTGGAACAGCGGAGATCGGAAG10.9615384615384616No Hit
GTGAAAACGAAAAAAATACCGAAATCCCAAAAGTATAGGAAATCATAAAA10.9615384615384616No Hit
ATTATCATATGCAGCATAAGTGGTGTTGAGTTAGTATTTGTGCTTCAAAA10.9615384615384616No Hit
ATCCCTTTGGAAGCTAATCCTCTGGAAAGTACGGTAGTAGCAAGATCGGA10.9615384615384616No Hit
GCTGTCTACTTTCATATATAGTACTTAAGCTGACTATCACTCCCACTTAA10.9615384615384616No Hit
AAGCAAGCCATCGGCTCTGATTTGCCAGGGGATGGCCGGAAAGATCGGAA10.9615384615384616No Hit
GGCTTCTTAGGGAACCGCACAGCTCGCTCGGACTTGTCTCTAATGGCTAT10.9615384615384616No Hit
CTTTTGTCAATTAATGCTGATATTGCCATACGTGGGTAGATCGGAAGAGC10.9615384615384616No Hit
ATATTTTCGTTAATTTTTAACATATTTTTTTATTCATGCATAAAATTCAA10.9615384615384616No Hit
GCGCTGGTGAAGAGTACCGATGGTGAGGAGTGAGATCGGAAGAGCGTCGT10.9615384615384616No Hit
CCGCCGCGTTGCTCGTCTTGGCTTGAGATGCAGATCGGAAGAGCGTCGTG10.9615384615384616No Hit
ACTGCCGATCGATGCAAATATGACGTCGGCTCAAGGATAAATGATATGTC10.9615384615384616No Hit
TCGGCTCTAATCTTGGGAATTCAGTTTCTGTTACAGATGGCTGATTGACA10.9615384615384616No Hit
CGCCTCCTGCTCTCCATGCTTGGTAGATCGGAAGAGCGTCGTGTAGGGAA10.9615384615384616Illumina Single End PCR Primer 1 (100% over 26bp)
TTCAAACAAATAAGACAATTTTTGAAGTATCAGATCGGAAGAGCGTCGTG10.9615384615384616No Hit
ACCGCACAATGGGCACAGGCGACACCGAAAGGACGAGGACGGGGCAGCGA10.9615384615384616No Hit
AAGAGGAGCATCAAGCACAAGAACAAGAACGGTTACAGCAAGATCGGAAG10.9615384615384616No Hit
AGAAGACTGATGTTAGAGCCATGGAGTATCCATACAAGTCAAAGGAAAAA10.9615384615384616No Hit
TTGGTTTCACTTTTGTGGCTTTCATTCGCTTGGTTGTTCAAACATAAGTG10.9615384615384616No Hit
GTGAGTTTGCCTGAAACGATAGGCAAAGGTAGAAGTTAATGGACCATATT10.9615384615384616No Hit
ATCCCCCAGACTAAAACAAATATTACAACTCCTAATTAGGCTAGCTAGCT10.9615384615384616No Hit
TTTTCCAAACATCGAACCCATTCCACAACAGTAAGTTCACAAGCTGTAGT10.9615384615384616No Hit
GACTTACTGGTTTGGCACCTTCTATCAAGGGAATCTGATGATCACAGTAA10.9615384615384616No Hit
TCCCATCGATCACAAGCAGTCTCAACTTTGCATGGTATAATAGATCGGAA10.9615384615384616No Hit
GTATTGTCATACGTGGAGGATGGAGGAAACATGAGAAATAACAGAGCAAG10.9615384615384616No Hit
TTCTCAGATCAGTAATGTCGAGTTCTATATGAATATAGCACAGATCGGAA10.9615384615384616No Hit
TACGGTGTTCGACGCTACGACCAAGCAGCAGCGATGCGGGGGAAGATCGG10.9615384615384616No Hit
GTTTAAGTGGCACACAGCTGCGACACGGCGGCACTAGGGCGACTTGGCTC10.9615384615384616No Hit
CGCTGACGATGATGATGACGACGGAAGATATGCCTCTCTTCAAACAGGAT10.9615384615384616No Hit
TGCCGCGCCGCCGCTTCAAGTTGCGCGCCGTCGCCTTCGGCCGCCGCCGC10.9615384615384616No Hit
ATAGTGATCGTCTTAAATCATTTCAATGGAAAGGTTGCAAGATCGGAAGA10.9615384615384616No Hit
TGGTAATTTAGAAACTTAGATCCATTCACGATTTGAAGATTGAGATCGGA10.9615384615384616No Hit
GTGCGTTCGTGGCAAAAACTCACTTCGTGATTCGCGCGGCGAACTTTTAT10.9615384615384616No Hit
TTCTGGCATTTGCCAGGTAGAATTGGTTGCAAATAGAACTCCTGCTCTGA10.9615384615384616No Hit
TAACTGTCCCTGAGGCAATGCAGCTTGTATCAACGCTTCTGGATACCGAG10.9615384615384616No Hit
TTGTTGAAAAACCGGTGCCGGCTATCCTTATGAACTGTGACAATCAGATC10.9615384615384616No Hit
GTGCGATGTTTTTGACCGGAAAGAAAAGGTTCAAAAAGCACCAAAACATG10.9615384615384616No Hit
CAAATGTTGCTAGATTTAAAACCCACTGTAAATAGAAAAATAGATCGGAA10.9615384615384616No Hit
AATAGGGTGATAAAAAAACTCTCAACATAGAGATCGGAAGAGCGTCGTGT10.9615384615384616No Hit
AATACCAGTCCGGATTCACATTTGCATTCTACAGAACCAGAAAATGAACT10.9615384615384616No Hit
TGGCTTGCCTTGCTGGCCTTGGAACTCTTCAAAGTCTTCTCCTGCGAAAT10.9615384615384616No Hit
CTGTTATGAACTCAATTTTCTGTCCTGCAAGACATAGATCGGAAGAGCGT10.9615384615384616No Hit

[FAIL]Adapter Content

Adapter graph