FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n02_HRB0333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n02_HRB0333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATTTTGTGAAAAAAGATTCGTTGTGAAAAAATAAAGGGGATGTAATA15.0No Hit
TGAAAAATCCCCAAATACCCCCCCCCTAGCCCATTTTTCTTTTTTCCTTC15.0No Hit
TGTATCCTCTAGCCTCGAAGCTGGCTGCAGCTGAAACTGAAGAACAATCA15.0No Hit
CTACTTGGGAGCGAGAAGATGAATTGAAGTCAGCTCATCCTCATCTAGAT15.0No Hit
TTCTCTTATTATCACCAGAAGACGAGATCACGATCCCAAAAGCACAGATC15.0No Hit
CGGGTCATTTAAAACACAGGATGCAGAGTCAAAGAATGAGAAGGAATCTC15.0No Hit
CATTGGATTCGATTAGTCGTCGCTTCGTTGTTGGATTCGTGGCCATCCAT15.0No Hit
CCCTGCCGCTTCTCCTCATCGGCCTTTCGGAATCGAGATCGGAAGAGCGT15.0No Hit
CGTGCCGCGGCAGCCATCAGGGTGGAGACGGCTTGAGGTGCCCGTGCTCT15.0No Hit
GGATGGAGAGGCGGGTGAGGCGAGGGGGGTCGCCCGCCGGCGGGGGAGAA15.0No Hit
GCGCCTCCTCGGCCTCCTCTCCGGAGATCGGAAGAGCGTCGTGTAGGGAA15.0Illumina Single End PCR Primer 1 (100% over 26bp)
AAAGAAATCAAGAAAGGGGGCGAGGTGGAGCAAGAGACCGTGTCCTCGAA15.0No Hit
TGTGGAGAGTGACAGACAGAGCGAAGAGCCTCCTCCAGAGATCGGAAGAG15.0No Hit
ATTTTGGGCCATGTAACTTAAGAAAAGATTCTCAAGATCGGAAGAGCGTC15.0No Hit
TGCCAACATTCGCATTAATAGACAAAAATTCGCCGCGCGAAAGATCGGAA15.0No Hit
TGGCCGCCTCCGCCTCCTGCTCGTGCCCGCACCGCCAACATCACAGATCG15.0No Hit
GCGCCGGCCGCGGCGAGGTCGCGAGATCGGAAGAGCGTCGTGTAGGGAAA15.0Illumina Single End PCR Primer 1 (100% over 27bp)
GCTAGTACCTACTCCCTCCGTTCCAAAAAAAGACAAACCCTGGTTTCCGT15.0No Hit
ATCCGCCTCCCGCCGCCGCCAGCGTAGATCGGAAGAGCGTCGTGTAGGGA15.0Illumina Single End PCR Primer 1 (100% over 25bp)
CCCCTCCTCTCTTCCCTCTCCCCTTTTTCCCCCTCTCTCAGATCGGAAGA15.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)