FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n02_HRB0330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n02_HRB0330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTTTGCCACAATCCGGTCTCAGGGTGTTCACCTCCTGCATGGAGTT11.1494252873563218No Hit
TATCTCTGTAGGTATCATATTTTGCCGCTCGTGTTTTGCCTCCTTCCAGT11.1494252873563218No Hit
CTCTGAGACACCCTAAGAGAGAAAAAGGGGGAGGGTAGAGGGGGAGAGGG11.1494252873563218No Hit
CGCCGCTGCTGTTATCTGGTGAGGAGAGAGGAGAGGAGAGGACGAGCGGC11.1494252873563218No Hit
TTACATAATGAGGATTTGAAATTATAAGACAGTTTTGGGCTTATGGTTTG11.1494252873563218No Hit
CAAGATAACCATATCTTATCATACAGTTTATCATGGTAGGAAGAGGGCAA11.1494252873563218No Hit
GATGTCTCCGATCTGTACCATCTGGACGCCCTCGACGTCTCTCTTCTAAG11.1494252873563218No Hit
GCGCGGCAGCTCTTGAGGGAGCAGGAGAAGTTGCTTCCTCAGGAGGAGGA11.1494252873563218No Hit
CCGGCGGAGGAAGAGAGAAAATGGAAAAAGAAGAAAGAAAGGGGAGGCTA11.1494252873563218No Hit
CCGCGCCCTCTCCTTCCTCTTCCCCGGAGATCGGAAGAGCGTCGTGTAGG11.1494252873563218Illumina Single End PCR Primer 1 (100% over 23bp)
TCCAACCTAAGAATTGCGATAATGTCCTGAAGTTCTTTGTAAAGATCGGA11.1494252873563218No Hit
TAAATGGCTGTCATGCGTCTAGAAGCGTTCCGAACTAGTATGAAGATCGG11.1494252873563218No Hit
TTGTTGGGGCCATTCCCGTTGCTGCTAGTGTGCACCATCTGGCATGGGCA11.1494252873563218No Hit
CGGCGTCAAACATTTTGGGATGGAGGGAGTACTATTTTTCCTCTATAAGA11.1494252873563218No Hit
CGGGCGCCGGCGTCGGAGAGGGATTCCGGGCGGGAGAGGGAGATCGGAAG11.1494252873563218No Hit
TTGTACAGAGCGACACGAGGCGAACCCAGGGAAAGCTCGGAGGGTAGGAC11.1494252873563218No Hit
TTGATTTCAGCTTTTTTTGAGAGGAACAAGTTGATTATTTATGCCCCAAA11.1494252873563218No Hit
TTTGCAACAGCTCAGTGGAAAATAAATTTTAGCAGATCGGAAGAGCGTCG11.1494252873563218No Hit
TTAATCTTTTTTGAAAGCTTAAAAGGGGGGAAGTAAACCTGTTTTTATTT11.1494252873563218No Hit
TAGACCTAATACTATATTTGTTTTGTTCAGTCATTTCTCACAGATCGGAA11.1494252873563218No Hit
AGGATCTCCTCTCCCATGTTTCTGCACCATCCAACCGAAAATTAGATCGG11.1494252873563218No Hit
CAAAAAGAAAACCCTAAAAGAAGTCTGTTTCCCCCCCCTTTCTCCCTCTA11.1494252873563218No Hit
TTAAAAAAAAACATAATTAACCAAAGAATATCACTACTTGGAGAAAAAAA11.1494252873563218No Hit
GAGCTCCAAGGTTACTGCCTCCTCCCTTTTAAAATAAATATAATTTCCTC11.1494252873563218No Hit
ATCTGATGTTTCAATACAATCATTAGAAAAAGAATAACAATAGGAAGATC11.1494252873563218No Hit
GGCCTTTCTGTTAAAAATGAAGAGTATTAATAATGATACAGATCGGAAGA11.1494252873563218No Hit
CTCATTCCATCCTTGATTGCTGCATCCTAGAATAGAGGTAGTAGTGAATA11.1494252873563218No Hit
TTGTCTCGTCTAATAAGACTAAACAATAGGATATCCTCTCGTTTCGTAGA11.1494252873563218No Hit
TGAAAGTTGCACCATCAACATTGTAAGGTTCAACCTGTTCTTTTGTTCTG11.1494252873563218No Hit
GGACGGATAGAGTATTGTTAGAAAATAAGAGAAAGATCGGAAGAGCGTCG11.1494252873563218No Hit
CGGAAAAGTCAATTTGCCGCACGAAAAAATAGTTGTTGTAGTAGTGATAG11.1494252873563218No Hit
TCGTCGCCCTCCTCCCTCTATTTATTCGCCCCACCCCTCGCTCGAGCTCG11.1494252873563218No Hit
ATTAAGGGACCACAAAAAATCTCGCTGTTAAGTTAAGGGACCTCCGATGA11.1494252873563218No Hit
CCCTGTCCCATCAAGATGATGGGAGACACAGCAACCGGAACGTCATTCCA11.1494252873563218No Hit
TTACCTGAATGTCAAAAAGAAAAATCGAAACGGTTACTTGCATTTATATA11.1494252873563218No Hit
ATAATCTCTTTGCTAGGTCATTACCCCTCTTAATAGTATGGCAAAGCTAT11.1494252873563218No Hit
GTACTTCTTGCTGTTTGTCATCATAGATCGGAAGAGCGTCGTGTAGGGAA11.1494252873563218Illumina Single End PCR Primer 1 (100% over 26bp)
ACCAAAATGATGAGATTAGTGCAGCTGTAATCAACTCGGGAGATGGTGAT11.1494252873563218No Hit
GCTTCCACTGCGTCCGTTAGTCGACTGGCAGCCTCGCGTAAGATCGGAAG11.1494252873563218No Hit
CTGTGCATGCGTATATGTTATACATCAAAATCATTAAATAAGCAGAATAA11.1494252873563218No Hit
AAAAATATTTTGCGTTACCTCTGCGTGAGCGCTCCGTTACTTTTCAAAAA11.1494252873563218No Hit
CTTCCTCGCGAGCCCAGACCCCACCGGGCGCCTTTGAAGAAAGGCTTGTC11.1494252873563218No Hit
GGAAAGAAGGAGACGGGAAGCAGAGACAGGAGATCGGAAGAGCGTCGTGT11.1494252873563218No Hit
ACAACAGTACTGGGGGGAAATCTATATGATCTCAGTTTGAATCTGCAAGA11.1494252873563218No Hit
TTTTTTGAAAAGGTCCATTTAAAAAAAAACGGGAGGGGGGAGAAGATCGG11.1494252873563218No Hit
GAGATGGCGGCCTGGCGCAGGGCGGCGGTGGGGGAAGGAGAGAGGAGAAA11.1494252873563218No Hit
GGTATGGAGCATGGACGAGCCCAACTGGTGCAAGGTTGGTGGACGCAGCG11.1494252873563218No Hit
CCTTTTCTGGGAGGTTGGGGATGAGGGCATACAGAATAGATCGGAAGAGC11.1494252873563218No Hit
CCTATCCTTTTTATTATTTCAGTTGATGATAAATCCTTTGGTTGACAGAT11.1494252873563218No Hit
TTACAATACTGCATATGAACTCTTTATTCCCAGCAATTTCTGGTTCCATA11.1494252873563218No Hit
GTTTTTTTTACTAACAGATTGAAGAAAAATCTATATGTGAAAGAAACAGA11.1494252873563218No Hit
TTCTCCATGCTTTCATGTGCTTTCTTCTCTTAAAAAGAAAAAAAAAATCC11.1494252873563218No Hit
TGGCTGTAGTAGGTGAGGGGGAGAGAGAGAAAAAAAGGGGTTAAATACTC11.1494252873563218No Hit
TTTTTCAAGGCTTAGCAGTGACCATTCGGTGCAGACTGAGATCGGAAGAG11.1494252873563218No Hit
TTTTGAAGCAACAAAATTGTCATTCTTCTTTTTTTTTTCTTTTTCAGTTT11.1494252873563218No Hit
TAAATAAAGTAGAAATAGGGTTAACACCTGAATTAGAGCTAGATTTTGGT11.1494252873563218No Hit
CGGAGAGGGAGAAAGGAAAAGGAGAATGAAAAACAAGATCGGAAGAGCGT11.1494252873563218No Hit
TTTTTTCTCCATTAGTGGGCCGCTTGTTAATAATATATAAGTTGATGAAA11.1494252873563218No Hit
GATTGTATCCATATTGGTTTTCCTAGTTCTACTTGGACAAAGATCGGAAG11.1494252873563218No Hit
CCACCTAAGATGACTAAGCTGTTTCAAATTTGCCATTTTAGCCAAAGATC11.1494252873563218No Hit
GATTTTGCTGGAGGGAGAAGAGAGGGAGATGGAAATGGAAATGGAAGAGA11.1494252873563218No Hit
CTTCCTTCCTCTCGCCATTATTCCCCTTGTCTTCGCCGCAGATCGGAAGA11.1494252873563218No Hit
TTAACCCTAGAATGGATAATATCTGGTGCCATATTATCTCTGAGATCGGA11.1494252873563218No Hit
CCGAGCCGCCGAGCCGTATTTGCTGGTTAATATTGTTTAATTATTACAGA11.1494252873563218No Hit
TGATAGCCAAGGAAACCACATAAAACTAAATTAAGCTAGTTTATCTACGG11.1494252873563218No Hit
AGATGGTCTATCATCTAGCCTCAACCGTGGTTTGGTGGAAGAGATCGGAA11.1494252873563218No Hit
TGTCTTTCCCTCAAAAGAAAATATGGAAGTTTAAGATTCCTCTGAAAATT11.1494252873563218No Hit
TAATGCCCCCTGTTGAGTCTACTCGAAGACGATGAAGCCCCCGCACTAAG11.1494252873563218No Hit
CTCTTGTGAAAATTTATTTTTAGGCCAGATAACTTTTCAAATACCGATAA11.1494252873563218No Hit
CTGGAGAGAGAGGGGGAGAGAGGGGAGAGAAAAGAGGGAGGGAGAGAGAG11.1494252873563218No Hit
CCTCACCTCACCTCTCTCTCTCTCTTCACTCTTTCTCTCTCTACATCTTT11.1494252873563218No Hit
GTTCCTCTTCAGCCCCGACGACGTCGCCGCGTTGCGCGACCAGCTCCCCA11.1494252873563218No Hit
CAATATGATTCCTTTGCATCTGATTAAATGCAATCTGAAGCGAGCAGCAA11.1494252873563218No Hit
CCTCTTTTTTCTCCTCCTTTTCTCTTCCAAATTTCTCTCCAGATCGGAAG11.1494252873563218No Hit
GGCAGCCTAAAGCCACGAACCAAACTCATACTAGAAGAGATCGGAAGAGC11.1494252873563218No Hit
GTCTTCATCTCCGATGTTGATATTGCCAAATTTGGAGATCGGAAGAGCGT11.1494252873563218No Hit
GCTACAGCATCATGAGATATAAGCTTTTGCAAACAGGATAGTCCTATTAA11.1494252873563218No Hit
CGAGAAAGCTGTGTGCACCCTGTGAAGGGAGATCGGAAGAGCGTCGTGTA11.1494252873563218Illumina Single End PCR Primer 1 (100% over 21bp)
CTTTGATTAAGCTGTATTGAATCTAACACATTGCAAATAACAAAAAATGA11.1494252873563218No Hit
TCGCAGATAGGTAACGCTTGTGTTCGGTATCTTGTCCAAATAGAATCCGA11.1494252873563218No Hit
AGTCCTCATGAGAGGATGGGGAAGGAGTGGGAGAAGATCGGAAGAGCGTC11.1494252873563218No Hit
TGGAAAAATTTCCCATTCCCCCTTTTCCCCCTTCTTTAGATCGGAAGAGC11.1494252873563218No Hit
CTGCGGGAGAGAGAAAAATAGATAAGGGAGAAGAGAAAAAGATAAAGTTG11.1494252873563218No Hit
TCTTGCTCAATTTTTCCCAAACCCAGAAGAGGAGTTCCTGGAAGATGAAG11.1494252873563218No Hit
GTTTCTCTCCTCCTTCCTTCTTCTTTCTCTCCCTCGGCTTCTCCCTTCCT11.1494252873563218No Hit
TCTAAAGAGGAAGGATGAAGAAGGAAGTGAAGATCGGAAGAGCGTCGTGT11.1494252873563218No Hit
CTCGAAGAAGAGAAAGAGAAGAGAGAAAAGAGAAGAAAAGAAAAGAAAAA11.1494252873563218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)