FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n02_HRB0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n02_HRB0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGAATATCTTCTTAGCCTCGTACGTTGTCTCGGGCAAAGATCGGAA10.9900990099009901No Hit
GCAACTTGAAAAATATGCAGACCGGTACATGAACTTAGCAATCAAGTGCA10.9900990099009901No Hit
TTCTGATCCAAATCTTTTGTCACCTTGCGCTCTCAACAGAAACAGAGAGC10.9900990099009901No Hit
TGGATCCACCCCTGGAGGAGCGAACCAGTGGAGCCGAGTAGGAGATCGGA10.9900990099009901No Hit
AGTCAAGAAGCAGCTTAATTCAAGTAATCAAAATAAGGCTCCGTTAGTCC10.9900990099009901No Hit
TTCATCATGTTGGCTTGCTGGGCGAGGATTTGGGTGAGAATGGCGTTGGT10.9900990099009901No Hit
AAATGTGTTAGCCTTGAAATTCTTGTTGTTTTGTTTCGAAGAGATGTCTG10.9900990099009901No Hit
TATATAATACCCGATGTGACATGCCAAAACTTTACACCCCTGGATCTAAA10.9900990099009901No Hit
ATAGATCGGTCACCTTGCCGAGACAGTATAAATCACTTACATCGAAAAGA10.9900990099009901No Hit
GCGCACCGCCCGTGCGCCATCTTCACTAAACCCTCCGCTAGAATCTTTCT10.9900990099009901No Hit
TGAATTAATATGATACAACATCGGCCCATCAACCAATGGACTATAGATCG10.9900990099009901No Hit
TCGACAAGCTATCACTAATCAAGGAAAAAGTTGACGAGTCACCAGATCGG10.9900990099009901No Hit
ACATTCTCTGGATCAATTTCAGGGGAATCTACGAACTAAGATCGGAAGAG10.9900990099009901No Hit
CGGACGAGTTCGTCGCCGTTGACTACTAGCCGTCTAGCACGTCACCGAAG10.9900990099009901No Hit
CTTATTACCTCAGAATCATGTCTCTTCAGCTCTGCAAGATCGGAAGAGCG10.9900990099009901No Hit
CACTCGAATTTGGAGTTCCACAAACCGATCTAGTGGTGAAACGTAGATCG10.9900990099009901No Hit
TCTTTTGCACGCTTCACTCAAAAAAGAACATCTAAGATCGGAAGAGCGTC10.9900990099009901No Hit
GATAGTCTCATTTTTCATGCAGAAGTAACATCTTAAGCTTCCATTCCAAT10.9900990099009901No Hit
GTTTAATTTTGCACTTCGTTTTCCAAACTTTTGTTTTTTTAACTACATCT10.9900990099009901No Hit
TCAATATATAAAATTCTCTTGCACCGATCATTTTTTCCATGTTTTATCTG10.9900990099009901No Hit
TGAATCAGTTCTACAATATCAAAGACTTAGCTCCTGAATTTTGCTAAGAT10.9900990099009901No Hit
GACAATTCTTAAGGACACATCCCTGCTTTCACTTAATTGAAGAGATCGGA10.9900990099009901No Hit
GCTCCTGCACCCATGGCATGGCGGCTTCAAAATCTTTGGATAAGATTATT10.9900990099009901No Hit
TGCTGGCCCGCGCATTCCAGGTCGACGGCGGCGTGGTCGAGAAAGATCGG10.9900990099009901No Hit
GATAAACAACATGAAACGTCAAAAATATCCCTAGGTCAGTCCTTCTCACA10.9900990099009901No Hit
ACGAGCACCTTGAAGAAGTGCGGCCGCCTCAGATCGGAAGAGCGTCGTGT10.9900990099009901No Hit
TTCACCGTGCTATATTTTTGGCTTGGCTTCGCAGATCGGAAGAGCGTCGT10.9900990099009901No Hit
AGTGTGCTTGCGGTGCCTCCGCCACTGTGAGATCGGAAGAGCGTCGTGTA10.9900990099009901Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAATATTCCTGGCCAATGATTTTTAAGGGAAAATTTTAATTCTCCCAT10.9900990099009901No Hit
TTTTAGGGTTTTTTTATTTTTTTGGCTGAAAAATCTCTCTTTTGCTAAAA10.9900990099009901No Hit
ACTAGGGTTTATGGTCTGTGGTTGGTCATATTCAATACCATTTGATGAGA10.9900990099009901No Hit
GTTCCACTTATACTTAGAGTATTATTTGTTTGCAGAGATTAACACATAAA10.9900990099009901No Hit
GAGATGAAAGGAGGAGAGGAAGCGGCGATGCTACCGGAAGATCGGAAGAG10.9900990099009901No Hit
GTAAAGATCAATTTTTTTAACCGGGATTAAAAATCATTTTTAATCCCGGC10.9900990099009901No Hit
ACTTTGCCTTTCGGTTATGGAATCGAGCTAGGATTTTTTTGACGGCAGAT10.9900990099009901No Hit
GGATTCGAGAACTGACTTTTGGGACGTTTATTTGGTAGCAAGGAATTAGG10.9900990099009901No Hit
CAACTGCTACCAGTGTGTTCATGCTGTAGATTGCCCTGAAAAGGATTATG10.9900990099009901No Hit
TCAGCCTCAATGTTTTGAGTCTGCATTTCATTATAAATCACTTCTACAGA10.9900990099009901No Hit
GCAAATTTCTACTAGAACTACAGCAGCACTGTGGCTGTACAGCCAAGATC10.9900990099009901No Hit
ATGTTTGTTTACAAATAGGCTTAGAAGGTCATTACACCTTCACACACGGC10.9900990099009901No Hit
CATCAGTTAGTTCCCTGATCCTGCTTTGCACACTACATGAGAAACGAAGA10.9900990099009901No Hit
CCCCTCGTGACCTCATCGCCGTTTCCCCCTTTTCAGATCGGAAGAGCGTC10.9900990099009901No Hit
GTACTACTAAAGTAGAATAGCATGAGAGAAGTTTCTTTCATTTTTCAACG10.9900990099009901No Hit
CATGAGGTAAAAAAGGGTCGACATTCATTAAGGTGATGATCAGGAACCTG10.9900990099009901No Hit
CCGGCTCGCGGCGGCCCGCCCCCGCCGCGCGATCCGCTCGCGCCTCGATC10.9900990099009901No Hit
CTTGTCCTCTGAGACTAAGTACAACACGTCAATAGTATCTTTCTGATGAG10.9900990099009901No Hit
ACAAAGCAATCTAAAAGCAAAAATTACTTCGCGCCGTGCACGCCGGCCGG10.9900990099009901No Hit
GATCTTTTGCTCGAGTATACAAGATGTACACATATAAAGAGATCGGAAGA10.9900990099009901No Hit
CCAGAACTCACACTTGCTGAGCTTGGCATACAATTGGTGTTCCCGCAACA10.9900990099009901No Hit
ACTCAGGACCTAAAGTTGTGGCTCCAGTTAGGCCTGTGGTAGAGAGATCG10.9900990099009901No Hit
GCATATCAATTTATCTCTATCGGCTGCTTCTCGCTTTAGGGTTTCTACCG10.9900990099009901No Hit
AAAGAAAAAGGTAAAACGCAACAAAAATTAAAGATGTAAAAGCAGAGATC10.9900990099009901No Hit
TGTTGAAAGCATCCTTATTCCACTCTCTCTTCCCTTACTCAAATTGTTCT10.9900990099009901No Hit
CTGTGTTTAGTTTCACGCCAAAATTGGAAGTTTGAAAAAAATTGGAAGAG10.9900990099009901No Hit
TGTAAAGCTCATTTTGTAATTCTTTTCATAAGCCTAAGAAGATCGGAAGA10.9900990099009901No Hit
TGGCGCCAGGATCTGCTGCGCCTCCGCCGGGCGACACCTCGCGCTCGGCA10.9900990099009901No Hit
TGGCTTCCAAGCCACGGACCGACGAACGTGCGGAGATCGGAAGAGCGTCG10.9900990099009901No Hit
AAAAGGGGGTCTATTAAGAACTTAATAAGGATTAGAAGTTGATTTGGGGT10.9900990099009901No Hit
CCCTAACACCCTTGTCACCCTCAGGTGAAGCCTCGGTATCCGTACCTCGC10.9900990099009901No Hit
CTTTGGCTGTTTGGCAGACTGTTTATGTACCCGTATGTTCGCAGATCGGA10.9900990099009901No Hit
AGGATCGCGGCTATGGTGGAGAGTATAGTGGACAGGCGCTTGAAGGAGAA10.9900990099009901No Hit
TGGTTAGTATATGGACGACACAAAGGACGTTAGGGAGATCGGAAGAGCGT10.9900990099009901No Hit
GTGTGTGTGTGTGTGTATGAAAATTGCTCTAAAATATCAGATAAATCCAT10.9900990099009901No Hit
GAGCGGAGTATGGCGTAGCATCTCGGTGTGCTCCGCGCGCTGCAGCCGTT10.9900990099009901No Hit
CGCGTGCTGGACTGAGCAAACTCTCAAGATCGGAAGAGCGTCGTGTAGGG10.9900990099009901Illumina Single End PCR Primer 1 (100% over 24bp)
ACATATGGAGCCCATATGTCAGTCCTTCTTCCTCCTCCCTCTCTCCTTTC10.9900990099009901No Hit
ATGTGTCAATCAATTGAAAATAATGAGTCCATCGATTTATGTCAAATCCG10.9900990099009901No Hit
CACCGGGACATGTATTACACTTGGCTAGACTCTAAATTCTTAACTTGAGA10.9900990099009901No Hit
CTCGCCGACACCATCTGGAACAGCGGCCGCTCGACGGCCTCGTCGGGGTC10.9900990099009901No Hit
GGAATTACTCCCCAATCATCGTCCTGACCTTTGGGCAAGAGATCGGAAGA10.9900990099009901No Hit
TATGTTTGAATCAAATAAATGTGTCGTATCTAAATATGGAACTTTTATTG10.9900990099009901No Hit
ACTGACATTTTTGTGAAAAGAGATGTTTGCGATGGCAATTTTAAAACAGT10.9900990099009901No Hit
CTTTGCCTACTTTACTGCCGTTGATGCGCAACTTCGTCATCATAGTCGAT10.9900990099009901No Hit
AGACAATGTGGATAAAAGACAGGCAATAAGATCTTAGGTAGGTTCCTTTT10.9900990099009901No Hit
CCATGTCAAACGGTCGAGACCTCACCGACGTCTGCTTCGACGTCGACGGC10.9900990099009901No Hit
TTGCTGGCTGGCTCTGGCTGTCTACGATGGGCGTCCATGGCAAGATCGGA10.9900990099009901No Hit
CTTCACACTTAGGGCCAGTTTAGTTCTCAAAAATTTTCCCCTTCACCTAT10.9900990099009901No Hit
AAGAACAGTCCAGTTTGGAAAGGGAGAGGGGCATGTGGTAGATCGGAAGA10.9900990099009901No Hit
TGAGAGAACACAAACAACGGCACGAGTCAATAAGATCGGAAGAGCGTCGT10.9900990099009901No Hit
CCAATTGTTCAATACTTCAATTTACATGGATTTTCAGAGTGCAGACCTTA10.9900990099009901No Hit
TTTAGTACTACCTCCGTCCTATAAAAATTGCATTTCTAGCTAATAGATCG10.9900990099009901No Hit
TGTTTCAAATATCTCAAAATCCTCTTATCCGCCGCCCATGGAAGAAGATC10.9900990099009901No Hit
TTTAATTGCGAGATTCGTTAAATCCTGCTCCATATTTATCGCTTGCATGC10.9900990099009901No Hit
CGGCGTCAATTCCCGCTCTGCTTATGTGTTGTACTGTATCTATTATTTCC10.9900990099009901No Hit
CATCATCACTTATGATGATGACCGGAGGATCTGCTAAGGCTGGGGCAACT10.9900990099009901No Hit
GATTGACAGGTGGGCCCCAGCACAGTAACTTTTTATAATTTCTCCATTTA10.9900990099009901No Hit
GTTTAGCCGCCAACAAAATAGTTGAGCTCTAAGGCTCTAAGGTGTTGCTC10.9900990099009901No Hit
GTGTCCAACAAAATTTGTGTGCCACACTTTGGAGATCGGAAGAGCGTCGT10.9900990099009901No Hit
CGTGGCCGCTCGTCTCCCTCCCCTTTGCAAGATCGGAAGAGCGTCGTGTA10.9900990099009901Illumina Single End PCR Primer 1 (100% over 21bp)
AACTTAATTTATTGAATTTTATTCCCTCATTTTGATGATCTTGAATTTTG10.9900990099009901No Hit
ATCGGGGAAAGATAGATGACGACGGTGAACCGGCGGCAACACATGGAGAC10.9900990099009901No Hit
GCACCGCTTCATCTTCGGGCAAGCTAATGTCCAAGAACTTGTCGCGCACC10.9900990099009901No Hit
GACAAAAGTTCGCCGCGCGAATCAAGAAGTAAGTTTTTGCCACGAACGCA10.9900990099009901No Hit
CTGTGACTAGCAGCCGAACTCATATAGGAGATCGGAAGAGCGTCGTGTAG10.9900990099009901Illumina Single End PCR Primer 1 (100% over 22bp)
TGATTTTCCTGGATGGCATGAGCGTTTGGAAATTCTGAAAGTTCACTCTA10.9900990099009901No Hit
GCGCCGTGAGACCTAGCGCCGCCGTCGCCGCCGTCGCTCCAGCCGTCGCC10.9900990099009901No Hit
TTCTTTTTGTTTTCCCTTAAAATAAAAAACCTTTCCAAGATTCTCGGTGA10.9900990099009901No Hit
TCCTCCTCTCTCTCCGCCGTCGCCGCAGATCGGAAGAGCGTCGTGTAGGG10.9900990099009901Illumina Single End PCR Primer 1 (100% over 24bp)
CCATCGTGCCCGTCGCCACCACCTCGTGACCGCGGCCACTACCAGGTCAC10.9900990099009901No Hit
CGAAATTAAAACCTTGATAACTTTGCCTTCCGGTCATGGAATCGAGCTAG10.9900990099009901No Hit
GAGACCAGGTCCCCGTGCCATTCGGTATTCCGAACTCGGAGATCGGAAGA10.9900990099009901No Hit

[FAIL]Adapter Content

Adapter graph