FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n02_HRB0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n02_HRB0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGTAGTTGAGAGGTTTCAGGAAAACGACCTCGGGCCAGCTGGGTAG11.3513513513513513No Hit
CTTCTTCCCAGTTACTCGTATTACTGCCCTACGAGTAGAAATTCAGATCG11.3513513513513513No Hit
AACAAGTCATTTTGACACATCCAGAGCACCTCCCTGGATGGGGGAAAACA11.3513513513513513No Hit
AATTTCTTTTTGTGTGAAACGATGGTATTTTGTTTTTTTCCCCCGAAGAA11.3513513513513513No Hit
AACACACACACATTATCGAGAACGTTCTCGATAAATGTGGAGTTGTCACA11.3513513513513513No Hit
TTTATCGTCTAACAAGCAGGTTTCTTTTAGACCAAATCCAAACTTACTAA11.3513513513513513No Hit
GAATCGGCCGATGGGAGTGAAGTCGCCACGATGTCGTGTTCTTGGTGGAG11.3513513513513513No Hit
CCATCTACGTCCTCTTCTAGCTCCTCTCCCGTCGTTCGCCACGCTCCGTC11.3513513513513513No Hit
CTCCCTCCCGATCTCTCCTCCCTTCGGATCCACCCTCTCTCGCTCCCCTC11.3513513513513513No Hit
TTGGAGGCGGAGAAGGTGTCGAGGGAGGAGAGATCGGAAGAGCGTCGTGT11.3513513513513513No Hit
AAATTCATGTTTGATCTGATGAATTTCACATTGTTGTGTCTACCTACCTA11.3513513513513513No Hit
CCTTCTTCCATTACAACGCACGGGTATTTTTTCTAGTGATATTAAAGATA11.3513513513513513No Hit
AGAGGAAATCTTACATAGCACAAAGGCATCAGGAAAAAGTGCTAGATGTC11.3513513513513513No Hit
CTCCTCTCGCCTCCCCCCACGCGCTCTCCCCCACAGATCGGAAGAGCGTC11.3513513513513513No Hit
GACTGAAACTGCAACTCTCTTTTGCTTGCAAGGGAGTATTCTAGATCGGA11.3513513513513513No Hit
GTTTTAATTATTTGAGTTATCATTATTCCAGTTGTTATCAATTTTTTTTA11.3513513513513513No Hit
CACCCCTCCCCTCCCCATGTCCTCGCCTTCCTCCCCGCGCTCTTGCTCCC11.3513513513513513No Hit
CTTCTTCCTCCTTCCTTCCTTCTTCTGCTTCTTAGATCGGAAGAGCGTCG11.3513513513513513No Hit
CTCTTGTGTTTTCCTTCGCGGAGATTACAGAAGCGGCCGCGTCATCGGAG11.3513513513513513No Hit
TCATCCGTCATCGCTGCGAGAGTGCGACACCTCTTTGTAGGTTTGTCTTG11.3513513513513513No Hit
TTATGTTCCATGGGCTACAATTGTCTTTTTACCGATGAGATCGGAAGAGC11.3513513513513513No Hit
GTTTGGAAATCACTGAAGATTTCACATATTCTCCATCACTGCAGTGCATA11.3513513513513513No Hit
CGTATTGTTCTTAAGAATCTAAAAGCAAAAAATTTAGAAAGTAAATTTCG11.3513513513513513No Hit
AATTTGGCAGCAAACTAAATAAAGCCACTTTTTTGGCAACTTTACCAAAA11.3513513513513513No Hit
CTGCCATCTCACCATCACTCAGTTCCCAGTAAGACACAGATCGGAAGAGC11.3513513513513513No Hit
GAACGGGGGATCCGTGCGTAAACCCTAGGGTTTTTGGGTGGGCGCTTGGG11.3513513513513513No Hit
AAGTTACTGCTACTCACTTGATTAAAGTTGCCTATCGAACTGGGAAATAG11.3513513513513513No Hit
CACATCTTCCTCCTCTCTCTCTCTCTCTCCCCCCTCTCTCTTCTATCTTC11.3513513513513513No Hit
CCTCCTCCCTCGGCCCGCTCGGCTCTCTCTCTTCCTCAAGTCTGCTTTAC11.3513513513513513No Hit
ACAAGCACAGCCTCAAAGAAAAAAACCTGCAATAGAGTTTCTTTTTTTTT11.3513513513513513No Hit
CCCGGGCCGCCCACCCTGTCGAGAGGAGATAAGTGAGAGAGAGAGGAAGG11.3513513513513513No Hit
CCGCCGCCCACCAACCGCCCCCGCGCGCCGTCCTCCCCCGCCCGCCCTGC11.3513513513513513No Hit
ACAAAAACTTCCAAAATTGGGTCAAAGATTCCGAAATATAGGATTTTAAG11.3513513513513513No Hit
CGGCATCGCCTCCTCCGGCTTCGCCGCACCGCCGTCGACCCCGTCCACCC11.3513513513513513No Hit
GGTTCATCAATGATATACTTGCATATGGTCTTGGTAGGCATAAGATGAAG11.3513513513513513No Hit
GACATACACCTCTCTTCCTCTTTCAAAACCCAGATCGGAAGAGCGTCGTG11.3513513513513513No Hit
ACGAAGGGAAAACGCGCTCGCCAAGCCACGACCCTAAGGCACGGCAGGCA11.3513513513513513No Hit
CTCGCCGTCCTCGGCTGGCTTTTCTATGTCAATTCTTGCCGCCGCTATCA11.3513513513513513No Hit
CTGAAGAAAGCATCTTCTGGAAAGTCCAAAACAGTAGATCGGAAGAGCGT11.3513513513513513No Hit
ATGTACTCGGATGCACCAGAAGTAAAGGAAAAATTGGTTGTGATGCATCC11.3513513513513513No Hit
AATAAAAAAGGCAGAGAAAATAAAGATAAACAGAGAGGAAGGAGAGATCG11.3513513513513513No Hit
CCGCCGCCTCGCTCCTCGTTACTCTCACCACCAAGATCGGAAGAGCGTCG11.3513513513513513No Hit
TGGTGAACTTATTTCTTCTTTTTTCTGGCCCAGTCTGGCCCAGAGCCAAT11.3513513513513513No Hit
CAGCACCCCTGTGGTCGTCCTCTTCCTCTTCTTATAGCTTTCTCCACTGC11.3513513513513513No Hit
CCTTCTGATTGCTGGTTGAAGCGGATACCGGTCTTCTGCTTGGTATCGCC11.3513513513513513No Hit
GTGAGAGCCGCCGCCGCCTCCCTCTCTAGCTCTCTCTCTTCGATCTCGAT11.3513513513513513No Hit
TCTATCGTTGAGTAGCGTGCCTGACACCATTCTTTTTTATCTTTACAGAG11.3513513513513513No Hit
GGAAGAGGGAGGAGGATGACGCCGGCGGGAGGAAAAAGGGGAAAAGAGGG11.3513513513513513No Hit
ATCCAGCAAGCCCCCACATCAACGCCGCTCCGGCCGCGCGCGACGCCGCC11.3513513513513513No Hit
GGAGAGGTGCATGGGTCAACTTATCTTAGATCGGAAGAGCGTCGTGTAGG11.3513513513513513Illumina Single End PCR Primer 1 (100% over 23bp)
CATGGCCTTTAGCTAGCCAGAGCAGATCAGCACAAGCAAAAGATCGGAAG11.3513513513513513No Hit
AGAGCTACTGCAGTACCGATTAAAAAAACGGTTGTAGCTACTGGGCGACG11.3513513513513513No Hit
CCTTGCTCTTGCTCTTGCGATTCTCTTCTTCTTCTTCCTTCGCCTTCAAT11.3513513513513513No Hit
GGAGAAGAAGAAGGAGAAGAGCTCGAGTTCGGAGATCGGAAGAGCGTCGT11.3513513513513513No Hit
TTTTGAAAAAAGAAAATAAAAGACTATTTCGGTTCCTATATAACTCTTAT11.3513513513513513No Hit
CTATTCTATACAGACTAACACTACATCATTAGATCGGAAGAGCGTCGTGT11.3513513513513513No Hit
AGGTTCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGTACAGATCGGAA11.3513513513513513No Hit
TCTGATTAGAGCGGAAAGGACCCGCGGAATAGATCGGAAGAGCGTCGTGT11.3513513513513513No Hit
TCTCTCCTCTTCTTCCTCTCTCTTTCTTCTTTTCTCTCTTTCTCTCCCTC11.3513513513513513No Hit
AGTACACCGGATCAAATCCCACCTCTTTTCCACCCCATGCAGCCAATCTC11.3513513513513513No Hit
TCGCTGCGCTCTAGGTTTTTTTTTTTCCTCTGCGCCTTCTCCCCGCAAGA11.3513513513513513No Hit
GTTGTTGTATGTGCAGGTGTTGCTTGAGGCCTTGGGAGAGAGATCGGAAG11.3513513513513513No Hit
ATCGACAATGGTATTGTATACTTGACCAACAAAGAAGATCCGAGAATAAC11.3513513513513513No Hit
CATTCTGCAAAGCAGCACTCTTTGAAGGTTGAAGGGTTGAAATATTATCA11.3513513513513513No Hit
GACCCAACTGACTATCGCAGCCTGGCTGGTGCACTTCAGTACTTGACAAG11.3513513513513513No Hit
CTGCTTGTTTATTGCTCTGGCCTGCAATTTTTTTTTTATATTTTCCCCTT11.3513513513513513No Hit
TTGCCTCCTGCGTGGCTATGTCCAGAGAGAGAGAGAGAGAGAGAGAGAGA11.3513513513513513No Hit
TCCCCTTGCTTGAAGGGCAAGGTCCTCCTTTTATAGATGAAGGAGATACC11.3513513513513513No Hit
CCTCCTCGCCTCCTCCCTCTCCAAGCACGCTTGCCGACCTCCCCGTCCAA11.3513513513513513No Hit
AGGACAAAATTGATTAATTGAAGCTAGTAGCCGACGCGAGATAAGATCGG11.3513513513513513No Hit
TATATGTGTCTCATTCACAAATGTGTAGCTTCCGTATCTAACAGTGCCGG11.3513513513513513No Hit
CAACTTTGTTCTTTCAGCAGCTATACAAAAAACAGATCGGAAGAGCGTCG11.3513513513513513No Hit
CACCATGTGAACATACAACTCTTGCCATATGTAACTCTGCGAGCTAGATC11.3513513513513513No Hit
GTTTTTTAATTCTTTTTCGGATTTTTTTTGTTTTTCGGCCACTTTTCTTT11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)