FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB16LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB16LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTC20132.00636942675159TruSeq Adapter, Index 7 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTAT12419.745222929936308TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATG7612.101910828025478TruSeq Adapter, Index 7 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG304.777070063694268TruSeq Adapter, Index 7 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGA284.45859872611465TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGC81.2738853503184715TruSeq Adapter, Index 7 (97% over 36bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGA60.9554140127388535TruSeq Adapter, Index 7 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATATCGTATGCCGTC60.9554140127388535TruSeq Adapter, Index 7 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCG50.7961783439490446TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATATCGTATG50.7961783439490446TruSeq Adapter, Index 7 (97% over 37bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTA50.7961783439490446TruSeq Adapter, Index 7 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTCTTC40.6369426751592357TruSeq Adapter, Index 7 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTCTT40.6369426751592357TruSeq Adapter, Index 7 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGT40.6369426751592357TruSeq Adapter, Index 7 (96% over 33bp)
GAAGAGCAAACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTC30.47770700636942676TruSeq Adapter, Index 1 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATTTCGTATG20.3184713375796179TruSeq Adapter, Index 7 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGAACGACCTCGTATGCCGTC20.3184713375796179TruSeq Adapter, Index 7 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATATCGTAT20.3184713375796179TruSeq Adapter, Index 7 (97% over 37bp)
ACACTCTTTCCCCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTAT20.3184713375796179TruSeq Adapter, Index 7 (96% over 26bp)
GAAGAGCACACATCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTC20.3184713375796179TruSeq Adapter, Index 1 (96% over 29bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGAACGAACTCGTATGCCGTC20.3184713375796179TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGGACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATG20.3184713375796179TruSeq Adapter, Index 1 (97% over 34bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GAAGAGCACACGTCTGAACTTCAGTCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCCCGTAT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
CGCTCTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGA10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GAACCCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG10.15923566878980894TruSeq Adapter, Index 7 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGCACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 10 (96% over 32bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGAACGAAATCGCATGCCGTC10.15923566878980894TruSeq Adapter, Index 7 (96% over 32bp)
ACACTCTTTCCCTACACGACGCTCTTCAGATCGGAAGAGCACACGTCTGA10.15923566878980894Illumina Single End PCR Primer 1 (96% over 32bp)
GAAGAGCACACGTCTGAACTCCCAGTCACAAGAACGATCTCGTATGCCGT10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCAGTCACTGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGA10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
AGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
CTGATAGGACTAGTGTTTTTATTTACAGTTTTTGTATTTAAAAACAATTG10.15923566878980894No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGACCTCGTATGCC10.15923566878980894TruSeq Adapter, Index 7 (97% over 35bp)
GAGGATTCCATGCATTGTGGCGCAATGGATCCCGATTGTATGCAGTGGAG10.15923566878980894No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGAATTCGTATG10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAAAACGATCTCCTATGCCGTC10.15923566878980894TruSeq Adapter, Index 5 (96% over 31bp)
ACGCTCGCTGCTGAGGTCCGTGAGAGGGCGCGAGATCGGAAGAGCACACG10.15923566878980894No Hit
GAGCACACGTCTGAACTCCAGTCACAAGAACGATATCGAATGCCGTCTTC10.15923566878980894TruSeq Adapter, Index 7 (96% over 29bp)
AAACCTTAGAGGTTCTGGCAAGGAGAGAGATCGGAAGAGCACACGTCTGA10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAACTCCAGATCGGAAGAGGACACGTCTGAACTCCAGTCACAAGAACGAT10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GGTCAGATTTCAAGTGTTCCATGCGGAATTTTGAAACTTGGAAAGCACCA10.15923566878980894No Hit
GTAAAGGTTGGAAGACTGGTTCGGGGTATAATTCTGCAACGGGTGAAGTA10.15923566878980894No Hit
CTTTAGTTCGGCGCTGCAGCCCGCGGAGATCGGAAGAGCACACGTCTGAA10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAGAGACACGTCTGAACTCCAGTCACAAGAACGATATCGTATGCCGTCT10.15923566878980894TruSeq Adapter, Index 1 (95% over 23bp)
CTTCATCCTCTGCGACGCCATCACCGAAGAGGTGTTGATTCGCTAGATCG10.15923566878980894No Hit
GAAGAGCACACGTCTGAACTCCAATCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
GAAGAGCACACGTATGAACTCCAGTCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
TCAGATCGGAAGAGCACACGTCTGAACTACAGTCACAAGAACGATTTCGT10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GAACTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGAT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
ACACTCTTTCCCTACACGTCTGAACTCCAGTCACAAGAACGATCTCGTAT10.15923566878980894TruSeq Adapter, Index 1 (95% over 23bp)
CAGTTGTGTTGTCAGCCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAAGAGCACACGTCTGAACCCAGTCACAAGAACGATCTCGTATGCCGTCT10.15923566878980894Illumina Paired End PCR Primer 2 (95% over 22bp)
CTCTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGACGA10.15923566878980894TruSeq Adapter, Index 7 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGCCACAAGAACGA10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACGGACTGCGCTTCGATGAAATGGACGCCGATGAGATCGGAAGAGCACAC10.15923566878980894No Hit
GACTTCAGAAGCCAAAGATGAGGCGGAGTTAAGATCGGAAGAGCACACGT10.15923566878980894No Hit
TGTCTGGGTTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGGCACACGTCTGAACTCCAGTCACAA10.15923566878980894TruSeq Adapter, Index 7 (96% over 25bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTGACAAG10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGTCGTC10.15923566878980894TruSeq Adapter, Index 7 (96% over 32bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTA10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
AATCGGAAGACACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGC10.15923566878980894TruSeq Adapter, Index 1 (95% over 24bp)
GAAGCCAAAGATGAGGCGGAGTTACGGACCTCATTCTGATCGGAAGAGCA10.15923566878980894No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATG10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
CCAGTCACAGATCGGAAGCGCACACGTCTGAACTCCAGTCACAAGAACGA10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCC10.15923566878980894TruSeq Adapter, Index 7 (97% over 35bp)
CACTATCTAGGGAACAGGCTGTCAATTTGGACGCAACCAGTCTAAGTTCA10.15923566878980894No Hit
CGTGTGACTGAGGGAATGGAGGAGGCTGGGGCATAAATGGGGATGGCAGA10.15923566878980894No Hit
GATCGGAAGAGACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGC10.15923566878980894TruSeq Adapter, Index 1 (95% over 23bp)
ACGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACAAGAACG10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
ACACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG10.15923566878980894TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTGCAGTCACAAGAACGATCTCGTATG10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATTTCGTAT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GAAAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTCT10.15923566878980894TruSeq Adapter, Index 7 (96% over 28bp)
AGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTCTTCT10.15923566878980894TruSeq Adapter, Index 7 (96% over 28bp)
GCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTCTTCTG10.15923566878980894TruSeq Adapter, Index 7 (96% over 27bp)
ACGATAGCAATGTTCTGCATGTAGATGACGACCATAGTACCCAAAAAGAT10.15923566878980894No Hit
AGATCGGAAGAGACACGTCTGAACTCCAGTCACAAGAACGATATCGTATG10.15923566878980894TruSeq Adapter, Index 7 (96% over 25bp)
GCGGTCTTTGCCGTGAAGTGATTCCTTGAAGTCAAAACAACGAGATCGGA10.15923566878980894No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATATCGTATGCCG10.15923566878980894TruSeq Adapter, Index 7 (97% over 34bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATG10.15923566878980894TruSeq Adapter, Index 7 (96% over 25bp)
AGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTCTTCA10.15923566878980894TruSeq Adapter, Index 7 (96% over 28bp)
GAAGAGCACACGTCTGAAGTCCAGTCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
GAAGAACACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
AAACTGTGACCCCGGACATGCCGAATTTGCGCCATTCTGGAAAGGACCCC10.15923566878980894No Hit
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAA10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GCCATTCTCCAAAGCTTTCATGACGGCGAGCGGATCGCGATGGACCGTAT10.15923566878980894No Hit
GATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
CATATTCAAGTTCTGTCCAAACTTTAAAATCTTTTTTGTTACCGCAGCAA10.15923566878980894No Hit
GAAAACGAGAGGGAAAACGAGACGGAACTTCAGCTGACTAGGGCTGCGAG10.15923566878980894No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGACCTCGTAT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACAAGAACGACCTCGTATG10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
GAATAGAATAGAACAGAATAGAATAGAACAGAACAGAACAGAATAGAATA10.15923566878980894No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGAACTCGTAT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GAACTGCAGTCTGACAGACATTCCCTGATAGAACAGATCTTTCAATAGGG10.15923566878980894No Hit
GAAGACACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTCT10.15923566878980894TruSeq Adapter, Index 7 (96% over 26bp)
CCAGTCACAATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGAT10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
CCCACGCCTGGCGTGGCTGTTCGTTGTGGCGGTCCTGGCCCGCGGGCGGC10.15923566878980894No Hit
GATCGGAAGACCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTATG10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
GCGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGA10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTC10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GAAGAGCATACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
AGATCGGAAGAGCACACGGTCTGAACTCCAGTCACAAGAACGATCTCGTA10.15923566878980894TruSeq Adapter, Index 7 (95% over 21bp)
GAAGAGCACACGACTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
AGATCGGAAAAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGTAT10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACAAGAACGATTTCGTATG10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACG10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGCCACAAGAACGATATCGCATGCCG10.15923566878980894TruSeq Adapter, Index 1 (96% over 31bp)
AAAGCCTTTAATCTTGTTGAATTCATCAACTGATCCAAGAGCCAGAATAG10.15923566878980894No Hit
GAAGAGCACACGTCTGAACTCTAGTCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
GGGCGTCGATCGATTGCTCGATATGACGCGCACGATGGTCAATGTCGGCG10.15923566878980894No Hit
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTCGT10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
CTCTTCCGATCGGAAGAGCACACGTCTGAAGTCCAGTCACAAGAACGATA10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
CCAGTCACAATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTACGAT10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
TGTGCTCTTCCGATCTGAAGAGCACACGTCTGAACTCCAGTCACAAGAAC10.15923566878980894TruSeq Adapter, Index 1 (97% over 34bp)
AAAGCGAATACCATCAAGCGCTCATTAAAATGGGCAGAGTAGAAGCGATG10.15923566878980894No Hit
GAACTCCAGTCACAGATCGGAAGAGCAGACGTCTGAACTCCAGTCACAAG10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTAGATCGGAAGAGACACGTCTGAAC10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GAAGAGCGCACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTC10.15923566878980894TruSeq Adapter, Index 1 (96% over 29bp)
GATAGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGA10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
GGTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACG10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)
CCAGTCACAGATCGGAAGAGCACACGTCTAAACTCCAGTCACAAGAACGA10.15923566878980894Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACGTCTGAACTCCAGTCACAAGAACGATCTCGTATGCCGTCTTCTGCTTG10.15923566878980894Illumina Paired End PCR Primer 2 (96% over 30bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAACGATCTC10.15923566878980894TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)