FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB16EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB16EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22370
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCGCCAACATCTCGTATGCCGTC9214.117121144389808TruSeq Adapter, Index 11 (96% over 31bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGC4051.8104604380867233TruSeq Adapter, Index 11 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCAACATCTCGTAT2621.1712114438980779TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCAACATCTCGTATG2331.041573535985695TruSeq Adapter, Index 11 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCAACA1640.7331247206079571TruSeq Adapter, Index 11 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCAACATCTCGTATGC1060.47384890478319175TruSeq Adapter, Index 11 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCCAACATCTCGTATGCCG420.18775145283862316TruSeq Adapter, Index 11 (96% over 33bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCAACA410.18328118015198927TruSeq Adapter, Index 11 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)