FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB15LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB15LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATGCCGTC7133.97129186602871TruSeq Adapter, Index 2 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTAT3014.354066985645932TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATG2913.875598086124402TruSeq Adapter, Index 2 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA2612.440191387559809TruSeq Adapter, Index 2 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACA125.741626794258373TruSeq Adapter, Index 2 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATGC52.3923444976076556TruSeq Adapter, Index 2 (97% over 36bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACA31.4354066985645932TruSeq Adapter, Index 2 (97% over 37bp)
TGACTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCT10.4784688995215311TruSeq Adapter, Index 2 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGATAACACCTCGTATGCCGTC10.4784688995215311TruSeq Adapter, Index 2 (96% over 32bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAAC10.4784688995215311TruSeq Adapter, Index 2 (97% over 37bp)
GAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATGCCGTCTTC10.4784688995215311TruSeq Adapter, Index 2 (96% over 29bp)
ATTCATTGTCCTCAAAAGTAAGCGTAATAAAATTATTTTCGTTTATGTAG10.4784688995215311No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC10.4784688995215311TruSeq Adapter, Index 14 (97% over 38bp)
AAACGTTGACAGTGACAGAAAGCAATCCGATATCGACAGCCAGTCTGCGA10.4784688995215311No Hit
CACAACTTACGGTGAGGGGTCACAACTTACGGTGAGTGGTCACAACTTAC10.4784688995215311No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATGCCG10.4784688995215311TruSeq Adapter, Index 2 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGCATGCCGTC10.4784688995215311TruSeq Adapter, Index 2 (96% over 32bp)
TCTCCTGCACCATCTACAACGAGATCAGATCGGAAGAGCACACGTCTGAA10.4784688995215311Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGTCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATGCCGTCT10.4784688995215311TruSeq Adapter, Index 4 (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGCATG10.4784688995215311TruSeq Adapter, Index 2 (97% over 37bp)
CCAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACATC10.4784688995215311TruSeq Adapter, Index 2 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATGCCGAC10.4784688995215311TruSeq Adapter, Index 2 (96% over 32bp)
TAGACTAGTAGCTTTAGTGTCACTGATGAGCCGCTCGTCCTTTGGTTACT10.4784688995215311No Hit
CTTAACCCACTGAGCAAGGCCAGGGATCGAAGCTGCAACCTCATGAGATC10.4784688995215311No Hit
GAAGAGGACACGTCTGAACTCCAGTCACTGATAACATCTCGTATGCCGTC10.4784688995215311TruSeq Adapter, Index 4 (96% over 31bp)
AAAATGCTAATAATGAAGAAAAGCATAATTTGGTTTTGCGTTATTATTCA10.4784688995215311No Hit
CCCGGGTGTCAGCGGCGCGGGCCTCCTCACCTCTAGCGCGATCGCGCTCA10.4784688995215311No Hit
GTCTGTTGCTACACCTCCTGAACGATTACCCTTTGGTGTCGCTTCGGTGG10.4784688995215311No Hit
TAGATGAATGGAGAAAGGCGTTTAGTCAGTTTAAGACTAAAAAAAGATCG10.4784688995215311No Hit
AGATCGGAAAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATG10.4784688995215311TruSeq Adapter, Index 4 (96% over 29bp)
TATGCCAATGGAACTGTCTGCTAGCTGTTGGACATAGAGGTCTGGAGTGC10.4784688995215311No Hit
GAAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTCGTATGCCGT10.4784688995215311TruSeq Adapter, Index 4 (96% over 32bp)
GTCATCAGGCCGGTTATGCCAACATGGTTGGTAACGTTCCTCGTCTGACT10.4784688995215311No Hit
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATAACATCTC10.4784688995215311TruSeq Adapter, Index 2 (97% over 37bp)
GAACTCCAGTCACAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT10.4784688995215311Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAAGAGCACCACGTCTGAACTCCAGTCACTGATAACATCTCGTATGCCGT10.4784688995215311TruSeq Adapter, Index 4 (95% over 24bp)
CTCCCGATTCTGTGGGGGGCAAGAAATATTATGTGAGTTTTAACGATGAT10.4784688995215311No Hit
GATCGGAAGAGCACACGTCTGAATCCAGTCACTGATAACATCTCGTATGC10.4784688995215311Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GACCGCCTCGACGTATGGCTCGAATCGAGGAGGGGCGAACGGCAGGACAG10.4784688995215311No Hit
ATCTGAATTTATTTTCATTCTATTGAATGGCAGCGTGCAGTATACTTTCA10.4784688995215311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)