FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB15EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB15EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTCGTATGC49020.745131244707874TruSeq Adapter, Index 9 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTCGTATGCCGTC803.3869602032176123TruSeq Adapter, Index 9 (96% over 32bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCT241.0160880609652836TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTCGTAT220.9314140558848433TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTCGTATG190.8044030482641829TruSeq Adapter, Index 9 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTCGTATGCCG90.3810330228619814TruSeq Adapter, Index 9 (97% over 34bp)
GGGGAGCACACGTCTGAACTCCAGTCACGCTCCGTATCTCGTATGCCGTC50.21168501270110077TruSeq Adapter, Index 9 (96% over 29bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTATATCGTATGC40.1693480101608806TruSeq Adapter, Index 9 (97% over 36bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTA40.1693480101608806TruSeq Adapter, Index 9 (97% over 37bp)
ATCGGAAGAGCACACGTCTAAACTCCAGTCACGCTCCGTATCTCGTATGC30.12701100762066045TruSeq Adapter, Index 6 (97% over 34bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCCGTA30.12701100762066045TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)