FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB13LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB13LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6569
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTATG440.6698127568884154TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTAT410.6241437052823869TruSeq Adapter, Index 16 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTATGCCG120.18267620642411325TruSeq Adapter, Index 16 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCTCGTATGCCGTC80.1217841376160755TruSeq Adapter, Index 16 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)