FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB13EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB13EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9934
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATGCCGTC1271.2784376887457216TruSeq Adapter, Index 22 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATG840.8455808335011074TruSeq Adapter, Index 22 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTAT230.23152808536339842TruSeq Adapter, Index 22 (97% over 39bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCA110.11073082343466881TruSeq Adapter, Index 22 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGTACGCATCTCGTATGCCG110.11073082343466881TruSeq Adapter, Index 22 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)