FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB12LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB12LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTATG542.4861878453038675TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTATGCCG321.4732965009208103TruSeq Adapter, Index 2 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTAT110.5064456721915286TruSeq Adapter, Index 2 (97% over 37bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTC100.46040515653775327TruSeq Adapter, Index 2 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTATGCCGTC80.3683241252302026TruSeq Adapter, Index 2 (96% over 32bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTA60.2762430939226519TruSeq Adapter, Index 2 (97% over 37bp)
AGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTATGCCGTCTTCT60.2762430939226519TruSeq Adapter, Index 2 (96% over 28bp)
CTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTT50.23020257826887663TruSeq Adapter, Index 2 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTA50.23020257826887663TruSeq Adapter, Index 2 (97% over 36bp)
GACTGGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATC40.1841620626151013TruSeq Adapter, Index 2 (97% over 37bp)
GTGACGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTAT30.13812154696132595TruSeq Adapter, Index 12 (96% over 33bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTAT30.13812154696132595TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)