FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB12EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB12EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10601
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCAGTATCTCGTATG830.7829450051881898TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCAGTATCTCGTAT480.452787472879917TruSeq Adapter, Index 10 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGCAGTATCTCGTATGCCG380.3584567493632676TruSeq Adapter, Index 10 (97% over 34bp)
CTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCAGTATCTC200.18866144703329873TruSeq Adapter, Index 10 (97% over 37bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCAGTA160.150929157626639TruSeq Adapter, Index 10 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGCAGTATCTCGTATGCCGTC120.11319686821997925TruSeq Adapter, Index 10 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)