FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB0341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB0341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24776
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTAT1620.6538585728123991TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGCCGTC1520.6134969325153374TruSeq Adapter, Index 18 (96% over 32bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1200.4843396835647401No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG660.26638682596060703No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC390.15741039715854052No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT360.145301905069422No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATG340.13722957701000968TruSeq Adapter, Index 18 (97% over 37bp)
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC270.10897642880206651TruSeq Adapter, Index 1 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)