FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB0340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB0340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7832
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTATGCCGTC1331.698161389172625TruSeq Adapter, Index 2 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTAT861.09805924412666TruSeq Adapter, Index 2 (97% over 37bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG500.6384065372829418No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA450.5745658835546475No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTATCTCGTATG350.44688457609805926TruSeq Adapter, Index 2 (97% over 37bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT260.3319713993871297No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC220.2808988764044944No Hit
GAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTA220.2808988764044944TruSeq Adapter, Index 2 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATCTTA120.15321756894790603TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)