FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB0327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB0327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6420
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTC4637.211838006230529TruSeq Adapter, Index 23 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATG570.8878504672897197TruSeq Adapter, Index 23 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTAT400.6230529595015576TruSeq Adapter, Index 23 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAACTCTATCTCGTATGCCGTC90.14018691588785046TruSeq Adapter, Index 23 (97% over 35bp)
GGGGAGCACACGTCTGAACTCCAGTCACCAACTCTATATCGTATGCCGTC70.10903426791277258TruSeq Adapter, Index 23 (96% over 32bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA70.10903426791277258No Hit
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCTA70.10903426791277258TruSeq Adapter, Index 23 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)