FastQCFastQC Report
Thu 18 Aug 2022
000000000-KG26R_l01_n01_nHRB0220.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_nHRB0220.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4814
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTAT621.2879102617366016TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATG470.9763190693809721TruSeq Adapter, Index 3 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATGCCG120.24927295388450355TruSeq Adapter, Index 3 (97% over 34bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAA70.14540922309929372TruSeq Adapter, Index 3 (97% over 37bp)
TGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAAT50.1038637307852098TruSeq Adapter, Index 3 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATGCCGTC50.1038637307852098TruSeq Adapter, Index 3 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGTCAATCTCGTATGC50.1038637307852098TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)