FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n01_HRB0342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_HRB0342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTAAGGTTGTCAATCAGCCATCTGTAACAGAAACTGAATTCCCAAGA10.9615384615384616No Hit
ATTATACCATGCAAAGTTGAGACTGCTCGTGATCGATGGGAAGATCGGAA10.9615384615384616No Hit
TGACATATCATTTATCCTTGAGCCGACGTCATATTTGCATCGATCGGCAG10.9615384615384616No Hit
ACCCTTGCAAGGATTCTTTTGTTTGAATCTCCAAACGATAGCTGTGAAAT10.9615384615384616No Hit
CGGCACGGGAGGCGGCGGCGGCGGCCGAAGGCGACGGCGCGCAACTTGAA10.9615384615384616No Hit
CTACCTACTCACCTCAACAAAATGGTGTAGTGGAAAGGAAAAATCGTACA10.9615384615384616No Hit
GTCAACCGTCACCGTCGATGTATTGCCGTAAAGATCGGAAGAGCACACGT10.9615384615384616No Hit
CATATCAATAATTTATAATTTATCACTACAAAGCAATCGCACAACACTTA10.9615384615384616No Hit
GTGGTCTAGGGATAAATTAAATATTTTAAAAAATAGGGTGACAGTTACTC10.9615384615384616No Hit
GTGCTATATTCATATAGAACTCGACATTACTGATCTGAGAAAGATCGGAA10.9615384615384616No Hit
GCATCTCAAGCCAAGACGAGCAGCGCGGCGGAGATCGGAAGAGCACACGT10.9615384615384616No Hit
AGGTTTATCGATCCCAAGGGGTACAGATCCATAATAGACATATAGAGATT10.9615384615384616No Hit
TACTATGAGTGCGGGTTAATTCAGACTACAGACACGAGATCGGAAGAGCA10.9615384615384616No Hit
TCTTCCCTCGGTATCCAGAAGCGTTGATACAAGCTGCATTGCCTCAGGGA10.9615384615384616No Hit
CCGCTGTTCCACCTGTCGCCTGCTCCGTTCCAGGAGATGTAGATCGGAAG10.9615384615384616No Hit
ATCTGTTCTTTACTTTTTCATGTTTTTAGACTGCGATTAGGTAGTTTTTG10.9615384615384616No Hit
CTCCATCTCCATCTCCATCGCTCCATCAATTTGGGTTTAGATCGGAAGAG10.9615384615384616No Hit
CGCCATCGCGAGGGACAAGAGGGGAGAGGAGCAGAGGGAAGATCGGAAGA10.9615384615384616No Hit
TCCGATTTCGCAGGAGAAGACTTTGAAGAGTTCCAAGGCCAGCAAGGCAA10.9615384615384616No Hit
ATGTCTTGCAGGACAGAAAATTGAGTTCATAACAGAGATCGGAAGAGCAC10.9615384615384616No Hit
ATGCTTTGATGTTTCAGGGAACTATTAGAAATGGGCGAGATGTTGCGATG10.9615384615384616No Hit
TTAATTGATAAAAGTTCGCCGCGCGAATCACGAAGTGAGTTTTTGCCACG10.9615384615384616No Hit
CGAGTGATGTTGCTGCGGGAGCGAGTGATCCTGTGGAGTTGCTCAAAAAC10.9615384615384616No Hit
ATACCTCGTTCATCCCCAATGCCTGTAACATGAGTAACTCACGATCAGAT10.9615384615384616No Hit
AATCTCAAGCTAGCTAGCCTAATTAGGAGTTGTAATATTTGTTTTAGTCT10.9615384615384616No Hit
ATCGGCTAACATCTGCTACCAAAAAGCAGTCGGTTAGGAGATCGGAAGAG10.9615384615384616No Hit
CTTATAGTTGTATTCGCGCGCTTGTATGGTTGTATTCGCCGCAGCGGTGC10.9615384615384616No Hit
CAATCATATATTCTCATAATATAGACGGGAATTTGGACTGTTTAGAGGAG10.9615384615384616No Hit
CCCTCCAATATGTCCAAAAAACATGTTTTGGTGCTTTTTGAACCTTTTCT10.9615384615384616No Hit
GATACTTCAAAAATTGTCTTATTTGTTTGAAAGATCGGAAGAGCACACGT10.9615384615384616No Hit
AGCCTACCTAACTAAGATTTGAATATCTAAAAGATTTCAAATAAAAAAGT10.9615384615384616No Hit
TGTTTAGAGGACTGATTTGGTACTGGTACACATTCAAATGACAACTTTTC10.9615384615384616No Hit
CCTAGCCGAGCCAAGTCGCCCTAGTGCCGCCGTGTCGCAGCTGTGTGCCA10.9615384615384616No Hit
TTTGCTCACCTTACAATCTTCCTCCTAAAGCCTGTAACCTTTTTGTTCCA10.9615384615384616No Hit
TAGTTGATTTAATTAGTATCATATTAAAAGTTTAAGTGGGAGTGATAGTC10.9615384615384616No Hit
CTAGCGTAGTTTTGTTTGACTACAGCTTGTGAACTTACTGTTGTGGAATG10.9615384615384616No Hit
GATGGCGAGGAAGGCATGTGGGTTAACCCTTATTGGAAATCAAAGATCGG10.9615384615384616No Hit
TGCTACTACCGTACTTTCCAGAGGATTAGCTTCCAAAGGGATAGATCGGA10.9615384615384616No Hit
ATAGCCATTAGAGACAAGTCCGAGCGAGCTGTGCGGTTCCCTAAGAAGCC10.9615384615384616No Hit
GGAGGAGTCGGTGCAGGAGCCCGAGGAGGAGTCGGTGCAGGAGCCTGAGG10.9615384615384616No Hit
TCCGGTTTTGAATTTTATGCATGAATAAAAAAATATGTTAAAAATTAACG10.9615384615384616No Hit
TGCTGTTGCGGATGCGAGAGTTTGGAGATAGGATGGGCGGCAGTGTAAGC10.9615384615384616No Hit
TCGTAATCCTCGTCAGTCTTGTCCTCAACTCCGACGATTTTTCTTTTGCC10.9615384615384616No Hit
ATCAGTATCATCCGTCGCATGCAGATGCAGTGCATCATCACACATCATCT10.9615384615384616No Hit
CAATCCGTTCGCGATCTTGTTGCTCACGCTGTAGTCGTTCTCGCTCCTGT10.9615384615384616No Hit
GATTGTCACAGTTCATAAGGATAGCCGGCACCGGTTTTTCAACAAAGATC10.9615384615384616No Hit
TTTAACAACAGAATAATTAAAACATAAACATATTCAAATTTACAATTTTG10.9615384615384616No Hit
TCGACGTACTGCAGCGCGGTGCAGTTGAGATCGGAAGAGCACACGTCTGA10.9615384615384616Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTGTCAGATAACGGCCTGCAGTGTCCTCAAGTGTAGAACGGAAGAGCA10.9615384615384616No Hit
AAAACCTTAATTGCGAAATTTGTTTCTTTGCTTGTTGTCTAGTGCCCGAG10.9615384615384616No Hit
GGTTGAGTGACAGAAGCTTTGCGAGGATCTGCTTTGCTTGCATATAAAAA10.9615384615384616No Hit
CTATGTTGAGAGTTTTTTTATCACCCTATTAGATCGGAAGAGCACACGTC10.9615384615384616No Hit
TGATAATATGGTCCATTAACTTCTACCTTTACCTATCGTTTCAGGCAAAC10.9615384615384616No Hit
CGTATCGAATCCGATTCAAACAGATCCCATTTCAGAGACAGATTTTGTCC10.9615384615384616No Hit
ATTTTTCTATTTACAGTGGGTTTTAAATCTAGCAACATTTGAGATCGGAA10.9615384615384616No Hit
TCCCCCGCATCGCTGCTGCTTGGTCGTAGCGTCGAACACCGTAAGATCGG10.9615384615384616No Hit
ACGAAGCAGTCCATCGATGGTGACAAAAACACCAGTCGGCGCCAGTGGAA10.9615384615384616No Hit
GAACATATATTAAACTAATAAACAAACCAAGATTTTACAATTACAAGATC10.9615384615384616No Hit
TTGTGATTGGTTGAGAAGAGAATGTTGGTGGAGAAGTTGTAAAGATCGGA10.9615384615384616No Hit
TCTGGGTTGGCATCCTGTTTGAAGAGAGGCATATCTTCCGTCGTCATCAT10.9615384615384616No Hit
AAGGTGATCATCCTTTTCATGCTTCTAATCTTAAATACATTCAAGTTGGA10.9615384615384616No Hit
TATGGAAAAGCAAATTTTATGTGTACATGGCCAAAAATTGATTTGGAACA10.9615384615384616No Hit
ATGGTTTATTCCCACACAACTATGGTTGTCATGGATAGTTCGGATGGAGA10.9615384615384616No Hit
GCGGTCAGCGACAGGTGATCTCGGCGGTTCGAATGGAGATCGGAAGAGCA10.9615384615384616No Hit
CTCCTGTGTTCACCTTCGCCAACCAAGCCGGCCTCGACATGAGATCGGAA10.9615384615384616No Hit
CTGTGAATTCAACTAAGGCATGTTCAAATTTGAATTTGTAGATCGGAAGA10.9615384615384616No Hit
CGCTTCGCCAAGCAAGCCCAAAACCTTTATGATCTCACCATCAAGAGGGT10.9615384615384616No Hit
TATTGCTGATAATTGGTGGTTGAAGTTTTCTAATACAAGCATGTCGGAAG10.9615384615384616No Hit
TCACTTGCTCTGTTATTTCTCATGTTTCCTCCATCCTCCACGTATGACAA10.9615384615384616No Hit
CGGATGAGGATTGGAGCACAGTTCTTTAGTTCAACCTAAGCATGAGATCG10.9615384615384616No Hit
AGATTTTTCCTTTGACTTGTATGGATACTCCATGGCTCTAACATCAGTCT10.9615384615384616No Hit
ATATATGATTCTGTGCTGTTGCTTTTGTTGCACTCCTCCCTCCTTTTGCT10.9615384615384616No Hit
ACCCACGTATGGCAATATCAGCATTAATTGACAAAAGAGATCGGAAGAGC10.9615384615384616No Hit
TGCAACCTTTCCATTGAAATGATTTAAGACGATCACTATAGATCGGAAGA10.9615384615384616No Hit
CTAACTTTTTCGCTGGGTGAGCTCTAACAAGTAACAACTAACAAGTCTTT10.9615384615384616No Hit
ATTGTACTATACCTTTTATGATTTCCTATACTTTTGGGATTTCGGTATTT10.9615384615384616No Hit
ACCAAGCATGGAGAGCAAGAGGCGAGATCGGAAGAGCACACGTCTGAACT10.9615384615384616Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGAAGACAAGATTCACTGGTGGAGAAACCATCTTTCGTCGGTCGGCCG10.9615384615384616No Hit
TTTGACTCCCTCGATGTTCTTGGAGAGTGGTTTCCCTGACGTGGTGTCCA10.9615384615384616No Hit
TAATGGCATTAATTGACAAAAGTTCGCCGCGCGAATCATGAAGTCAGTTT10.9615384615384616No Hit
CAATCTTCAAATCGTGAATGGATCTAAGTTTCTAAATTACCAAGATCGGA10.9615384615384616No Hit
CGTCTGTCGCTAGCGGCACGCACCCTGAATGAAAAGAAACCAAAGATCGG10.9615384615384616No Hit
AGCTACCTCCAGTCTAACTACGCCTCCTTGGTGTTAGATCGTGGTTGCTC10.9615384615384616No Hit
TTGCGTTGCGCCTTAGCGATCGATTCACCTCTCCGTGGGTTGTTAATCTT10.9615384615384616No Hit
TTGTAACCAGTCACCCGATGTTCCCAACCTCTTTCCCTCGTTTTCCGCGC10.9615384615384616No Hit
TGCATAAACAAGTAAGCAAGTGAGCTCAGAGAGTGGTACTGGAGTACTAC10.9615384615384616No Hit
TGCTGTAACCGTTCTTGTTCTTGTGCTTGATGCTCCTCTTAGATCGGAAG10.9615384615384616No Hit
GTTGCTTTGAAATATCGAACAAATCTATTTTTTAAATTTATAATATTAGA10.9615384615384616No Hit
ATCCAGCTGGCCCTGACATTGCATGATCGAGAGTTTTGATAGCGAGATCG10.9615384615384616No Hit
TTCCGGCCATCCCCTGGCAAATCAGAGCCGATGGCTTGCTTAGATCGGAA10.9615384615384616No Hit
TTTTTGACTAGTTCATTTTCTGGTTCTGTAGAATGCAAATGTGAATCCGG10.9615384615384616No Hit
AAAGCACCAAAACATGATTTTTGGACATATAGATCGGAAGAGCACACGTC10.9615384615384616No Hit
CATATTTATGTTGATTTTCATTTTTTTGCAGGTCCACACCTAGATCGGAA10.9615384615384616No Hit
CGCTTGCATGTTTAAGCACTAGGCCATGATAATTGGCTCCCCACCCCGGG10.9615384615384616No Hit
CCCAGAACCACAAAACCGGACATCTCCTTCAACCCCCAACTTTACATACA10.9615384615384616No Hit
TACTGTGATCATCAGATTCCCTTGATAGAAGGTGCCAAACCAGTAAGTCA10.9615384615384616No Hit
ATCGCACCCACCCGCCAAGTTCTTGTACCGTCAACGCAATTTACTCTATT10.9615384615384616No Hit
CACTCCTCACCATCGGTACTCTTCACCAGCGCAGATCGGAAGAGCACACG10.9615384615384616No Hit
AGCAATCAGAGCAGGAGTTCTATTTGCAACCAATTCTACCTGGCAAATGC10.9615384615384616No Hit
CGACGACATGCCTGCGTTGCTTGATCTCTAGCTAGCTTGTTCGAGATCGG10.9615384615384616No Hit
GTAGCATCTCTAAGCATTTGTCATAGTTGACTAGGGAAGATCGGAAGAGC10.9615384615384616No Hit
CCCCCTCTGGTTGGCTTAGTTCTTGTGATTCGAAGATCGGAAGAGCACAC10.9615384615384616No Hit
GATCTCTCTCCTCTCGCTGCCCCGTCCTCGTCCTTTCGGTGTCGCCTGTG10.9615384615384616No Hit
GTAGGGGACACCCCTCATATCAACCCGCGCGCCGCAGCAAATCCAAGATC10.9615384615384616No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)