FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n01_HRB0333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_HRB0333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTGATTGTTCTTCAGTTTCAGCTGCAGCCAGCTTCGAGGCTAGAGGAT15.0No Hit
GTGATGTTGGCGGTGCGGGCACGAGCAGGAGGCGGAGGCGGCCAAGATCG15.0No Hit
CGCGACCTCGCCGCGGCCGGCGCAGATCGGAAGAGCACACGTCTGAACTC15.0Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCGCGCGGCGAATTTTTGTCTATTAATGCGAATGTTGGCAAGATCGGAA15.0No Hit
AGATGAGGATGAGCTGACTTCAATTCATCTTCTCGCTCCCAAGTAGAGAT15.0No Hit
ACGCTGGCGGCGGCGGGAGGCGGATAGATCGGAAGAGCACACGTCTGAAC15.0Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGCTGCTTGAGATTCCTTCTCATTCTTTGACTCTGCATCCTGTGTTTTA15.0No Hit
GTCAAACGTTGGACACGGAAACCAGGGTTTGTCTTTTTTTGGAACGGAGG15.0No Hit
TCGAGGACACGGTCTCTTGCTCCACCTCGCCCCCTTTCTTGATTTCTTTA15.0No Hit
CGATTCCGAAAGGCCGATGAGGAGAAGCGGCAGGGAGATCGGAAGAGCAC15.0No Hit
TGAGGAGAGCACGGGCACCTCAAGCCGTCTCCACCCTGATGGCTGCCGCG15.0No Hit
ATCTGATGGATGGCCACGAATCCAACAACGAAGCGACGACTAATCGAATC15.0No Hit
GTGCTTTTGGGATCGTGATCTCGTCTTCTGGTGATAATAAGAGAAAGATC15.0No Hit
CTGGAGGAGGCTCTTCGCTCTGTCTGTCACTCTCCACAAGATCGGAAGAG15.0No Hit
TCGACGAGGAGGGCGAGAAGAAGAAGAGAGAAGACGCGAGAAGAGGGGGC15.0No Hit
CTGCTCTTCTCCCCTGCCGGCCGTCGGACCTCCTCTCCTCTCCCGCCTCT15.0No Hit
GAGAGAGGGGGAAAAAGGGGAGAGGGAAGAGAGGAGGGGAGATCGGAAGA15.0No Hit
ATTACATCCCCTTTATTTTTTCACAACGAATCTTTTTTCACAAAATATTA15.0No Hit
CCGGAGAGGAGGCCGAGGAGGCGCAGATCGGAAGAGCACACGTCTGAACT15.0Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGAATCTTTTCTTAAGTTACATGGCCCAAAATAGATCGGAAGAGCACA15.0No Hit

[FAIL]Adapter Content

Adapter graph