FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n01_HRB0330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_HRB0330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTTCCATTTCCATTTCCATCTCCCTCTCTTCTCCCTCCAGCAAAAT11.1494252873563218No Hit
CAGTCTGCACAGAATGGTCACTACTAAGCCTTGAAAAAAGATCGGAAGAG11.1494252873563218No Hit
AGCCTCCCCTTTCTTTCTTCTTTTTCCATTTTCTCTCTTCCTCCGCCGGA11.1494252873563218No Hit
TTGGCTAAAATGGCAAATTTGAAACAGCTTAGTCATCTTAGGTGGAGATC11.1494252873563218No Hit
CGGAGGAAGAGGGTACTCGTTTCCAAGAAAATAAAAACAGGTTTACTTCC11.1494252873563218No Hit
GCGCCGAAGCGGTCCTACCCTCCGAGCTTTCCCTGGGTTCGCCTCGTGTC11.1494252873563218No Hit
AATAAAGACTTTAATTTTCAGAGGAATCTTAAACTTCCATATTTTCTTTT11.1494252873563218No Hit
TGCAGATTCAAACTGAGATCATATAGATTTCCCCCCAGTACTGTTGTAGA11.1494252873563218No Hit
TCTCCCCCCTCCCGTTTTTTTTTAAATGGTCCTTTTCAAAAAAAGATCGG11.1494252873563218No Hit
ATTTTATGTAATGGAGGAAATTATATTTATTTTAAAAGGGAGGAGGCAGT11.1494252873563218No Hit
CTTCTAGTATGAGTTTGGTTCGTGGCTTTAGGCTGCCAGATCGGAAGAGC11.1494252873563218No Hit
TTATTCTGCTTATTTAATGATTTTGATGTATAACATATACGCATGCACAG11.1494252873563218No Hit
ATTCTGTATGCCCTCATCCCCAACCTCCCAGAAAAGGAGATCGGAAGAGC11.1494252873563218No Hit
TTGCCCTCTTCCTACCATGATAAACTGTATGATAAGGTATGGTTATCTTG11.1494252873563218No Hit
GGAGAGAAATTTGGAAGAGAAAAGGAGGAGAAAAAAGAGGAGATCGGAAG11.1494252873563218No Hit
AAAGATGTAGAGAGAGAAAGAGTGAAGAGAGAGAGAGAGGTGAGGTGAGG11.1494252873563218No Hit
CTCGAGGAGAAGGAAACGCGAGCACCGGGGCCGAGGCGCGTGGGGAGCTG11.1494252873563218No Hit
AGTGTTTTCCCAAAGAGAGTGTAATCGGTCGGATTCTATTTGGACAAGAT11.1494252873563218No Hit
TAACAGAGAAGGAATCAGAGCAGAACAAAAGAACAGGTTGAACCTTACAA11.1494252873563218No Hit
GGGGTGGCAAAGGTGCGAGCGAGCTCGAGCGAGGGGTGGGGCGAATAAAT11.1494252873563218No Hit
TAAAAAAAAAGAGATGGCAAACTGTAGTTTTCATCCAGGACAAACCATAA11.1494252873563218No Hit
TATAGAGGAAAAATAGTACTCCCTCCATCCCAAAATGTTTGACGCCGAGA11.1494252873563218No Hit
TACGCGAGGCTGCCAGTCGACTAACGGACGCAGTGGAAGCAGATCGGAAG11.1494252873563218No Hit
GCACATCTTTTTTTTCTTTTCTTTTCTTCTCTTTTCTCTCTTCTCTTTCT11.1494252873563218No Hit
TGTTTTTCATTCTCCTTTTCCTTTCTCCCTCTCCGAGATCGGAAGAGCAC11.1494252873563218No Hit
CCTCCTCCTCCCCACCGATGCCGCCCCTTTCTCCTCTCTCCTTCCCCCAC11.1494252873563218No Hit
TGGACTCCCTCTCCCCCTCTAACCTCTCTCTTTTTCTCTCTTAGGGTGTC11.1494252873563218No Hit
ATTTGTTGAGCCCACTTGACATCTCTCTCTCCCTCCCTCTTTTCTCTCCC11.1494252873563218No Hit
AACATCCATATATAAATGCAAGTAACCGTTTCGATTTTTCTTTTTGACAT11.1494252873563218No Hit
TCTCCCACTCCTTCCCCATCCTCTCATGAGGACTAGATCGGAAGAGCACA11.1494252873563218No Hit
AAAGAAGGGGGAAAAGGGGGAATGGGAAATTTTTCCAAGATCGGAAGAGC11.1494252873563218No Hit
CCGGGGAAGAGGAAGGAGAGGGCGCGGAGATCGGAAGAGCACACGTCTGA11.1494252873563218Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCACTACTACAACAACTATTTTTTCGTGCGGCAAATTGACTTTTCCGAG11.1494252873563218No Hit
ACGAAACGAGAGGATATCCTATTGTTTAGTCTTATTAGACGAGACAAAGA11.1494252873563218No Hit
GCTAAAATTTATTTTCCACTGAGCTGTTGCAAAAGATCGGAAGAGCACAC11.1494252873563218No Hit
CTCTTCTTGTGATCCTGCCCATGCCAGATGGTGCACACTAGCAGCAACGG11.1494252873563218No Hit
TTTAGTTTTCATTTTTTTCTCCAAGTAGTGATATTCTTTGGTTAATTTTG11.1494252873563218No Hit
AATTTTCGGTTGGATGGTGCAGAAACATGGGAGAGGAGATCCTAGATCGG11.1494252873563218No Hit
TAGTGCGGGGGCTTCATCGTCTTCGAGTAGACTCAACAGGGGGCATTAAG11.1494252873563218No Hit
TAGAGAGAGAGCCGAGAGAGGGGGAGGAAGGGAGAAGCCGAGGGAGAGAA11.1494252873563218No Hit
GTCAACCAAAGGATTTATCATCAACTGAAATAATAAAAAGGATAGGAGAT11.1494252873563218No Hit
TTTATTTATAGCTTTGCCATACTATTAAGAGGGGTAATGACCTAGCAAAG11.1494252873563218No Hit
CAGAGATAATATGGCACCAGATATTATCCATTCTAGGGTTAAAGATCGGA11.1494252873563218No Hit
CCTTCATCTTCCAGGAACTCCTCTTCTGGGTTTGGGAAAAATTGAGCAAG11.1494252873563218No Hit
TCCTATTGTTATTCTTTTTCTAATGATTGTATTGAAACATCAGATAGATC11.1494252873563218No Hit
GTAATAATTAAACAATATTAACCAGCAAATACGGCTCGGCGGCTCGGAGA11.1494252873563218No Hit
AAACTGATTTTATCGGTATTTGAAAAGTTATCTGGCCTAAAAATAAATTT11.1494252873563218No Hit
TTGTCCAAGTAGAACTAGGAAAACCAATATGGATACAATCAGATCGGAAG11.1494252873563218No Hit
TTGTTTTAATTGTTCTTTAAACAATAAAAGAAAATCTATTAAATATTTAG11.1494252873563218No Hit
CCTGTCTCTGCTTCCCGTCTCCTTCTTTCCAGATCGGAAGAGCACACGTC11.1494252873563218No Hit
ATAGTATTTCTCTATTCACTACTACCTCTATTCTAGGATGCAGCAATCTA11.1494252873563218No Hit
GGAATGACGTTCCGGTTGCTGTGTCTCCCATCATCTTGATGGGACAGGGA11.1494252873563218No Hit
TTACAAAGAACTTCAGGACATTATCGCAATTCTTAGGTTGGAAGATCGGA11.1494252873563218No Hit
TTATTACTCATATCCGTAGATAAACTAGCTTAATTTAGTTTTATGTGGTT11.1494252873563218No Hit
TTCTCTTATTTTCTAACAATACTCTATCCGTCCAGATCGGAAGAGCACAC11.1494252873563218No Hit
TCATACTAGTTCGGAACGCTTCTAGACGCATGACAGCCATTTAAGATCGG11.1494252873563218No Hit
ATTCCATCGATAACTCCATGCAGGAGGTGAACACCCTGAGACCGGATTGT11.1494252873563218No Hit
AGCTAAGCCAAGGAGTGGGCGTCTCATTTGGGGCATAAATAATCAACTTG11.1494252873563218No Hit
CCAAATTTGGCAATATCAACATCGGAGATGAAGACAGATCGGAAGAGCAC11.1494252873563218No Hit
ATTCCTCTGATTCCTCCTCCTCCTCTATGGGCTCCTCCTCCTGAGGAAGC11.1494252873563218No Hit
AGAGGGAGAAAGGGGGGGGAAACAGACTTCTTTTAGGGTTTTCTTTTTGA11.1494252873563218No Hit
TAATAGGACTATCCTGTTTGCAAAAGCTTATATCTCATGATGCTGTAGCA11.1494252873563218No Hit
CCCTTCACAGGGTGCACACAGCTTTCTCGAGATCGGAAGAGCACACGTCT11.1494252873563218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGAGTTAACAATATTTCATCAACTTATATATTATTAACAAGCGGCCCA11.1494252873563218No Hit
CCTTTGAAGCTGAAATTTTGTGCTGCATTTGCATCACCATCTCCCGAGTT11.1494252873563218No Hit
GTGTAATATTTTGCTGCTCGCTTCAGATTGCATTTAATCAGATGCAAAGG11.1494252873563218No Hit
CTTCCACCAAACCACGGTTGAGGCTAGATGATAGACCATCTAGATCGGAA11.1494252873563218No Hit
TCATCGTATTTCTTTATGGAACCAGAAATTGCTGGGAATAAAGAGTTCAT11.1494252873563218No Hit
GTCTTGCACCCTTTGTCAACTATACTGGAAGGAGGCAAAACACGAGCGGC11.1494252873563218No Hit
TCCAACTTTATCTTTTTCTCTTCTCCCTTATCTATTTTTCTCTCTCCCGC11.1494252873563218No Hit
TTTCATGAGAGATTGCCGGATTGCTCCACAAAAATGAACTAGCAGCGTGG11.1494252873563218No Hit
TCTTTCCCTCCTCTTTTTTTTCTTTCTTTTCAAAAGGATTTTTTTTTTCT11.1494252873563218No Hit
GCGGCGAAGACAAGGGGAATAATGGCGAGAGGAAGGAAGAGATCGGAAGA11.1494252873563218No Hit
ATGATGACAAACAGCAAGAAGTACAGATCGGAAGAGCACACGTCTGAACT11.1494252873563218Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGAGAAATGACTGAACAAAACAAATATAGTATTAGGTCTAAGATCGGAA11.1494252873563218No Hit
CTTGTACTACGTATTGTCAAAACTGAAAAAGAAAAAAAAAAGAAGAATGA11.1494252873563218No Hit
CTCGTTTCACAAGTATTTGATTCATTTTTTGTTATTTGCAATGTGTTAGA11.1494252873563218No Hit
CGAGTATTTAACCCCTTTTTTTCTCTCTCTCCCCCTCACCTACTACAGCC11.1494252873563218No Hit
GAAGATCAGTACGCTGCGTCCACCAACCTTGCACCAGTTGGGCTCGTCCA11.1494252873563218No Hit
GTCCAAATAACGCTTTTTTGAAGAGGTGACGATTTTTGAAAAGTAACGGA11.1494252873563218No Hit
TCACGCCGCTCGTCCTCTCCTCTCCTCTCTCCTCACCAGATAACAGCAGC11.1494252873563218No Hit
GGAATAAGTTCATCGGAGGTCCCTTAACTTAACAGCGAGATTTTTTGTGG11.1494252873563218No Hit
TCGGGTCGCGCCGACGGCTGGACAAGCCTTTCTTCAAAGGCGCCCGGTGG11.1494252873563218No Hit
TCCCTTCCTTCTTCATCCTTCCTCTTTAGAAGATCGGAAGAGCACACGTC11.1494252873563218No Hit
CTTAGAAGAGAGACGTCGAGGGCGTCCAGATGGTACAGATCGGAGACATC11.1494252873563218No Hit
GTATCATTATTAATACTCTTCATTTTTAACAGAAAGGCCAGATCGGAAGA11.1494252873563218No Hit
CCCTCTCCCGCCCGGAATCCCTCTCCGACGCCGGCGCCCGAGATCGGAAG11.1494252873563218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)