FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n01_HRB0327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_HRB0327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTACGAGCTGCTGCTCAACCCCGGCGCGACCAACAGCGAGAACGAGCC15.555555555555555No Hit
CCTGTCCTTCGAGGCCCGGGCGGACAACCGGTACGAGAGCAGCGCCACGG15.555555555555555No Hit
CCTTCCCGCTTGGCAAACAACCATTGGATGTTGCACCAGATCGGAAGAGC15.555555555555555No Hit
CAGTGTGGGTATGGGGTACCCATAATCTCCACAGTAGCCCCTGAGACAGA15.555555555555555No Hit
TATATTACTTGTAATGGTCAAAGCCTAAGCAGGGGTTAAGAGATCGGAAG15.555555555555555No Hit
CATCCCGAATTCGACCTCGTTGACCGACTCGGTGGCGCGCTTCATCAAGT15.555555555555555No Hit
GGAGCTACTGTTTTCTCCAAGATAGATCTTCGATCAGGATATCATAGATC15.555555555555555No Hit
TTGGATTTATGTCGTGCCAATCCAACATAAGCCTTTATTTTTTTGAGTAG15.555555555555555No Hit
CTTCTGATTTCCACACTGCTAGCCATGAAAAGCCCATATTGATGGAATTC15.555555555555555No Hit
AAATCTGGTTTCAGATTTGAATTATAGATCATAACTGTAAACATTTGTGC15.555555555555555No Hit
CCCTAACGTCCTATGTGCCATCCCTATACTAACCACCTAGATCGGAAGAG15.555555555555555No Hit
ATTAAGAATTGTAATTCCCGCAGCAAAGCGTGGGGAATCACCTAAGATCG15.555555555555555No Hit
AGGGAAAAAAATTCCTCACCTTATCCTTCCAGCGCTTCATCTCGCCCTCC15.555555555555555No Hit
TGCATTTCACAGCGTCGCGCCCGCCAGCCTACCTTAACGGCGAGATCGGA15.555555555555555No Hit
ATTGGAGGCGAACTAGATGGCGATGGCTTGATGGGAGGAGAAGAGATGGA15.555555555555555No Hit
TCAAAATCAGCCTCTAGGCCGATTTAGACCAATATGAGGATAACTACCCG15.555555555555555No Hit
TAAAAGCATCCTGCTAGGCAAGTTTTAATATAGCTAATGTATAATATTTA15.555555555555555No Hit
AAAAGGAGAGGAAGGAAGGAGGAAGCCGGGAGGAAGAAGGAAAAAGAAAA15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)