FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n01_HRB0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_HRB0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTGCCGTCGACGTCGAAGCAGACGTCGGTGAGGTCTCGACCGTTTGA10.9900990099009901No Hit
TTCGATGTGATAGGTGAAGGGGAAAATTTTTGAGAACTAAACTGGCCCTA10.9900990099009901No Hit
TAGTTCGTAGATTCCCCTGAAATTGATCCAGAGAATGTAGATCGGAAGAG10.9900990099009901No Hit
ATAGTCCATTGGTTGATGGGCCGATGTTGTATCATATTAATTCAAGATCG10.9900990099009901No Hit
TCCAGATGACCAGATGTAGTTAAAAAAACAAAAGTTTGGAAAACGAAGTG10.9900990099009901No Hit
TGCATAATCCTTTTCAGGGCAATCTACAGCATGAACACACTGGTAGCAGT10.9900990099009901No Hit
CTTCCAATTTTTTTCAAACTTCCAATTTTGGCGTGAAACTAAACACAGAG10.9900990099009901No Hit
CCTATAAACCCTTCTCCTTCAAGCGCCTGTCCACTATACTCTCCACCATA10.9900990099009901No Hit
ACCACATGCCCCTCTCCCTTTCCAAACTGGACTGTTCTTAGATCGGAAGA10.9900990099009901No Hit
AAACCCGCGATCGACGAAAATCCGATAACCGCAACTATACGCAAACAATC10.9900990099009901No Hit
CTTTATATGTGTACATCTTGTATACTCGAGCAAAAGATCAGATCGGAAGA10.9900990099009901No Hit
TCTCTAGGAAAAGGAACCTACCTAAGATCTTATTGCCTGTCTTTTATCCA10.9900990099009901No Hit
TCGGTGACGTGCTAGACGGCTAGTAATCAACGGCGACGAACTCGTCCGAG10.9900990099009901No Hit
CATTTTGTAAACTCAAGTTTTACCCAGGTGATCAATTTTAAAGTTTGAGG10.9900990099009901No Hit
GAAACTATCTGGGATTTTTCATCGGATATCCGATATTTTAGCAAAAGAGA10.9900990099009901No Hit
ATCTTGTTTGGACCTAGTGTCGTCGTCGTCTCCATGTGTTGCCGCCGGTT10.9900990099009901No Hit
ATTGGCACCTGTAAGGTCTGCACTCTGAAAATCCATGTAAATTGAAGTAT10.9900990099009901No Hit
TGCCATGGACGCCCATCGTAGACAGCCAGAGCCAGCCAGCAAAGATCGGA10.9900990099009901No Hit
CCGTACAGGAAATAATAGATACAGTACAACACATAAGCAGAGCGGGAATT10.9900990099009901No Hit
TATTGACTCGTGCCGTTGTTTGTGTTCTCTCAAGATCGGAAGAGCACACG10.9900990099009901No Hit
CAATTTGAACCGACGTTCCAATCATCCCTAATTCCTTGCTACCAAATAAA10.9900990099009901No Hit
TTCTCGACCACGCCGCCGTCGACCTGGAACGCGCGGGCCAGCAAGATCGG10.9900990099009901No Hit
GGGGCGTGCAGCGCAGCATCCGGGGCAGCCACCGGTGACGTGGATCGAGG10.9900990099009901No Hit
GCCGTCAAAAAAATCCTAGCTCGATTCCATAACCGAAAGGCAAAGTAGAT10.9900990099009901No Hit
CACAGTGGCGGAGGCACCGCAAGCACACTAGATCGGAAGAGCACACGTCT10.9900990099009901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGACGGGGAGGAAACGGCTGCAGCGCGCGGAGCACACCGAGATGCTA10.9900990099009901No Hit
ATTTAGCAAGACGAAATTGGAATGGAAGCTTAAGATGTTACTTCTGCATG10.9900990099009901No Hit
TCAAGCTTCCGTTGTCATAACCTTTTCCAATAAAAGTTCCATATTTAGAT10.9900990099009901No Hit
CCCTAACGTCCTTTGCGTCGTCCATATACTAACCAAGATCGGAAGAGCAC10.9900990099009901No Hit
ACGTTTCACCACTAGATCGGTTTGTGGAACTCCAAATTCGAGTGAGATCG10.9900990099009901No Hit
GGTTCGTGGCGGCGAGGTTATGGTGGGCGCAGTGGTGGTGGGTGCGGCAG10.9900990099009901No Hit
TGCAGAGCTGAAGAGACATGATTCTGAGGTAATAAGAGATCGGAAGAGCA10.9900990099009901No Hit
TTAAATCATGTATGCATGCAAGCGATAAATATGGAGCAGGATTTAACGAA10.9900990099009901No Hit
CCTAGGAGCCTAGCAAGGGAGCTGAGGGGAGCCGGACCAAGCCGCCACAG10.9900990099009901No Hit
CCGTCTTTCGTCACCGAGAATCTTGGAAAGGTTTTTTATTTTAAGGGAAA10.9900990099009901No Hit
TTCCATTAAAAGCCGGGATTAAAAATGATTTTTAATCCCGGTTAAAAAAA10.9900990099009901No Hit
TCGTCGGCTCCGACCCCGACGAGGCCGTCGAGCGGCCGCTGTTCCAGATG10.9900990099009901No Hit
ACAGTACAATTGAAAGCATCATCTGTAGAAGTGATTTATAATGAAATGCA10.9900990099009901No Hit
TGAGAGTTTGCTCAGTCCAGCACGCGAGATCGGAAGAGCACACGTCTGAA10.9900990099009901Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGATATAGCGCAACCCCAAATCAACTTCTAATCCTTATTAAGTTCTTAA10.9900990099009901No Hit
GCGAACATACGGGTACATAAACAGTCTGCCAAACAGCCAAAGAGATCGGA10.9900990099009901No Hit
CATCGAGTACCGCGCCCGACATGCCGAGCGCGAGGTGTCGCCCGGCGGAG10.9900990099009901No Hit
GCTGTCACAAAAAACCTAGCTCGATTCCATGACCGGAAGGCAAAGTTATC10.9900990099009901No Hit
TCCTGCCACCGGCCGGCGTGCACGGCGCGAAGTAATTTTTGCTTTTAGAT10.9900990099009901No Hit
GAAAAGGGGGAAACGGCGATGAGGTCACGAGGGGAGATCGGAAGAGCACA10.9900990099009901No Hit
GTGTTTTTTTACGATGAAGGGGGTGTTTAGATCCAGGGGTGTAAAGTTTT10.9900990099009901No Hit
CCAAAGTGTGGCACACAAATTTTGTTGGACACAGATCGGAAGAGCACACG10.9900990099009901No Hit
GGTGACTCGTCAACTTTTTCCTTGATTAGTGATAGCTTGTCGAAGATCGG10.9900990099009901No Hit
GTTGCGGGAACACCAATTGTATGCCAAGCTCAGCAAGTGTGAGTTCTGGA10.9900990099009901No Hit
TAACCAAATAATCTTATCCAAAGATTTTGAAGCCGCCATGCCATGGGTGC10.9900990099009901No Hit
GAAATTCCACTGGAGAATGACTGTTTTAAAATTGCCATCGCAAACATCTC10.9900990099009901No Hit
CGGGTGCGATGTTTTTGACCGGAAAGAAAAGGTTCAAAAAACACCAAAAC10.9900990099009901No Hit
CCGCACGTTCGTCGGTCCGTGGCTTGGAAGCCAAGATCGGAAGAGCACAC10.9900990099009901No Hit
CCTCCTCCACCACCACCACCAGACACCAACGCCATTCTCACCCAAATCCT10.9900990099009901No Hit
GAGAGAAAGAGAGAGAAGGGAGAGAGGGAGGAGGAAGAAGGACTGACATA10.9900990099009901No Hit
ATGACGACCTTCCCGAGCGACGGCGACGGCTGGAGCGACGGCGGCGACGG10.9900990099009901No Hit
CTTGCCCAAAGGTCAGGACGATGATTGGGGAGTAATTCCAGATCGGAAGA10.9900990099009901No Hit
CTTGAAGAAATGGATTTATCTGATATTTTAGAGCAATTTTCATACACACA10.9900990099009901No Hit
AGAAAGCCGTGTGTGAAGGTTTAATGACCTTCTAAGCCTATTTGTAAACA10.9900990099009901No Hit
TTTCGATGTAAGTGATTTATACTGTCTCGGCAAGGTGACCGATCTATAGA10.9900990099009901No Hit
TGGCTGTACAGCCACAGTGCTGCTGTAGTTCTAGTAGAAATTTGCAGATC10.9900990099009901No Hit
CATCAGAAAGATACTATTGACGTGTTGTACTTAGTCTCAGAGGACAAGAG10.9900990099009901No Hit
TCGTTTCTCATGTAGTGTGCAAAGCAGGATCAGGGAACTAACTGATGAGA10.9900990099009901No Hit
GCGGCGACGGCGGAGAGAGAGGAGGAAGATCGGAAGAGCACACGTCTGAA10.9900990099009901Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCAAAGGGGAGGGAGACGAGCGGCCACGAGATCGGAAGAGCACACGTCT10.9900990099009901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCTCGAGCTGCTCGCGCGGCGAGAGCGGGCCGCCGCGTGCCGGAGATC10.9900990099009901No Hit
CCTATATGAGTTCGACTGCTAGTCACAGAGATCGGAAGAGCACACGTCTG10.9900990099009901Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGATGTTCTTTTTTGAGTGAAGCGTGCAAAAGAAGATCGGAAGAGCACA10.9900990099009901No Hit
CTTCAATTAAGTGAAAGCAGGGATGTGTCCTTAAGAATTGTCAGATCGGA10.9900990099009901No Hit
CTATCAATTGGCGCCAAAGGTCCGTGTGGTGGAGAAAGAAGTTGCCCCAG10.9900990099009901No Hit
TCATTTCCAGAACAATTTGAGTAAGGGAAGAGAGAGTGGAATAAGGATGC10.9900990099009901No Hit
CTCACCTCTCTAGAGTGAACTTTCAGAATTTCCAAACGCTCATGCCATCC10.9900990099009901No Hit
AGATGATCGACTATGATGACGAAGTTGCGCATCAACGGCAGCAAAGTAGG10.9900990099009901No Hit
GCGAAGCCAAGCCAAAAATATAGCACGGTGAAAGATCGGAAGAGCACACG10.9900990099009901No Hit
CAGCTTAAGCCCATTAATCCCAGTGATCTCCTTGAAAAAAGAACCTTCAA10.9900990099009901No Hit
CCTACTCGGCTCCACTGGTTCGCTCCTCCAGGGGTGGATCCAAGATCGGA10.9900990099009901No Hit
CTGCTTTTACATCTTTAATTTTTGTTGCGTTTTACCTTTTTCTTTAGATC10.9900990099009901No Hit
AATCCATCTCTGAACATTTCCAGGGCACAGTAGCTCCAAGCTAACCGTTG10.9900990099009901No Hit
CAAGTTAAGAATTTAGAGTCTAGCCAAGTGTAATACATGTCCCGGTGAGA10.9900990099009901No Hit
CATCCAACCATCGGATTTGACATAAATCGATGGACTCATTATTTTCAATT10.9900990099009901No Hit
TCCGGTAGCATCGCCGCTTCCTCTCCTCCTTTCATCTCAGATCGGAAGAG10.9900990099009901No Hit
AGATTGGATTTTGAATTAAATGGAGAAATTATAAAAAGTTACTGTGCTGG10.9900990099009901No Hit
TTCCTTGACTGTATAACATGTAACAGGTTCCTGATCATCACCTTAATGAA10.9900990099009901No Hit
CATCAAATGGTATTGAATATGACCAACCACAGACCATAAACCCTAGTAGA10.9900990099009901No Hit
TTGCCCGAGACAACGTACGAGGCTAAGAAGATATTCTGCCCAGATCGGAA10.9900990099009901No Hit
CAACCGTAAGGCACACTATCTTAGCAAAATTCAGGAGCTAAGTCTTTGAT10.9900990099009901No Hit
ATTAGCTAGAAATGCAATTTTTATAGGACGGAGGTAGTACTAAAAGATCG10.9900990099009901No Hit
GCACGCATTTTTGATGATGGGTAAACAAAGTCCCAACAACCTGCAAGCTA10.9900990099009901No Hit
GCTTGCTTAGGAGTGAGTTTTCTTCATTTCTTCTCGTTTATGTGTTAATC10.9900990099009901No Hit
CTCTACCACAGGCCTAACTGGAGCCACAACTTTAGGTCCTGAGTAGATCG10.9900990099009901No Hit
GCTTACAAGCAAAATTCCGATCGGACTAACGGAGCCTTATTTTGATTACT10.9900990099009901No Hit
TCATTTCCAAGCGCACGGTGCGCGACAAGTTCTTGGACATTAGCTTGCCC10.9900990099009901No Hit
TAGACCTCACTCTTCTGGTAGTTGATTTTCATTCCAGACATCTCTTCGAA10.9900990099009901No Hit
AAAGAAACATAATATCTACAGAATCAAGCCGTTGACTGATTTTCAGGGTG10.9900990099009901No Hit
CCGAGTTCGGAATACCGAATGGCACGGGGACCTGGTCTCAGATCGGAAGA10.9900990099009901No Hit
CTGTTTTAAAAAGAGAAGAGCTCTCTGTTTCTGTTGAGAGCGCAAGGTGA10.9900990099009901No Hit
GAGGCGGCCGCACTTCTTCAAGGTGCTCGTAGATCGGAAGAGCACACGTC10.9900990099009901No Hit
TTCATCATGATTCTGTGTTTAAGAGCAACACCTTAGAGCCTTAGAGCTCA10.9900990099009901No Hit
TATGTATGTAACAATGTTTTGACCAAAATGCACTTGATTGCTAAGTTCAT10.9900990099009901No Hit
TCTTCCATGGGCGGCGGCTAAGAGGATTTTGAGATATTTGAAACAAGATC10.9900990099009901No Hit
TCTTAGGCTTATGAAAAGAATTACAAAATGAGCTTTACAAGATCGGAAGA10.9900990099009901No Hit

[FAIL]Adapter Content

Adapter graph