FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n01_HRB0233.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_HRB0233.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTTGAGGCGCAGCAGTTGAC111.11111111111111No Hit
TATTAAATCTGAAGTATTTAGCAAGCTAAGATTTTTAAAAAGAGCTAGCT111.11111111111111No Hit
AATGGCTTCTTACCCATCCAGCAAAGAGATAGGAAGAGCACACGTCTGAA111.11111111111111Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCTCTATGCATGTTAAGAATGTGCTCCTCTGGCCTCCTGTCGATATCTCC111.11111111111111No Hit
TTAATTCGTAAACAAGCAGTAGTAATTCCTGCTTTATCAAGATAATTTTT111.11111111111111No Hit
GGAGTAAAGCTTATGTTGATCTTTTTTCCCGGTTGGTAACACCAACCGGG111.11111111111111No Hit
CCTCTACAGACAAAAAGAAGCCTGCTACAGATAGTGACCCATCTGCTTTT111.11111111111111No Hit
CTACTCTATATTAAGATATTAAAGCAGGCAAAATATATCAAACATGAAGC111.11111111111111No Hit
AGCCGGGCTTTATATAAATCCTTTATCTAAAATCAAAGCTAGTCTTGACT111.11111111111111No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)