FastQCFastQC Report
Tue 16 Aug 2022
000000000-KG26R_l01_n01_HRB0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG26R_l01_n01_HRB0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGGCGATACCAAGCAGAAGACCGTTATCCGCTTCAACCAGCAATCAGA11.3513513513513513No Hit
GGAGAGAGATAGGGAAGATAGAAGAGAGAGGGGGGAGAGAGAGAGAGAGA11.3513513513513513No Hit
CATCGGTAAAAAGACAATTGTAGCCCATGGAACATAAAGATCGGAAGAGC11.3513513513513513No Hit
TTGAATATGATGATAATATTTCAACCCTTCAACCTTCAAAGAGTGCTGCT11.3513513513513513No Hit
GGGCGAGAGATGGGGGATCGGGAGGGGAGCGAGAGAGGGTGGATCCGAAG11.3513513513513513No Hit
CTCCTTCCTCTCTGTTTATCTTTATTTTCTCTGCCTTTTTTATTAGATCG11.3513513513513513No Hit
CTCCTCCCTCGACACCTTCTCCGCCTCCAAAGATCGGAAGAGCACACGTC11.3513513513513513No Hit
TGGCTAGCGGGGAAAAGTGACGGAGCGTGGCGAACGACGGGAGAGGAGCT11.3513513513513513No Hit
CGGAGAATATGCACTGCAGTGATGGAGAATATGTGAAATCTTCAGTGATT11.3513513513513513No Hit
GTAAAGCAGACTTGAGGAAGAGAGAGAGCCGAGCGGGCCGAGGGAGGAGG11.3513513513513513No Hit
GCGAAGGAGGCGAGCGGAGGAGGCGAGCGCGGCCTCGCAGGGCGGGCGGG11.3513513513513513No Hit
GAATTTCTACTCGTAGGGCAGTAATACGAGTAACTGGGAAGAAGAGATCG11.3513513513513513No Hit
TCTGCCCGGTGGCTTTCGGGACGACCCGGTTGCCTGCCGTGCCTTAGGGT11.3513513513513513No Hit
AGACTTGGCATTGAAGGCGAAGGAAGAAGAAGAAGAGAGTCGCAAGAGCA11.3513513513513513No Hit
TATCTCGCGTCGGCTACTAGCTTCAATTAATCAATTTTGTCCTAGATCGG11.3513513513513513No Hit
ATTCCGCGGGTCCTTTCCGCTCTAATCAGAAGATCGGAAGAGCACACGTC11.3513513513513513No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT11.3513513513513513No Hit
CCACCCAATCCTGCATGCAATGGTTCGAAAGATCCAAACAGTAAGTTTGA11.3513513513513513No Hit
CAATAATGTAGTACAATTTTTGCATGTGTTCTTCGGGGGAAAAAAACAAA11.3513513513513513No Hit
GTTTTTCTTGTTTTCCCCCATCCAGGGAGGTGCTCTGGATGTGTCAAAAT11.3513513513513513No Hit
TCTTCGTTGGTAGGTTGGGTGTGACAACTCCACATTTATCGAGAACGTTC11.3513513513513513No Hit
AATGATGTAGTGTTAGTCTGTATAGAATAGAGATCGGAAGAGTACACGTC11.3513513513513513No Hit
TTTACGAAGAAACAACAAGAAAAATTCATATTCTGATACATAAGAGTTAT11.3513513513513513No Hit
ACATAGCGACATCTAGCACTTTTTCCTGATGCCTTTGTGCTATGTAAGAT11.3513513513513513No Hit
GTTCATGTAGATCCCAAATTATCTTGTTGTAAGGATGCATCACAACCAAT11.3513513513513513No Hit
ACTGTTTTGGACTTTCCAGAAGATGCTTTCTTCAGAGATCGGAAGAGCAC11.3513513513513513No Hit
TATCAAAGGGGAAAATATAAAAAAAAAATTGCAGGCCAGAGCAATAAACA11.3513513513513513No Hit
ACTAGAGACTGAGGGGCTGTTCACTTTGGTGCCATTTTCAATCTTACCAA11.3513513513513513No Hit
AGAATACTCCCTTGCAAGCAAAAGAGAGTTGCAGTTTCAGTCAGATCGGA11.3513513513513513No Hit
ACCCAGCTGGCCCGAGGTCGTTTTCCTGAAACCTCTCAACTACATCAGAG11.3513513513513513No Hit
CTAGTGCGGGGGAAGCGTGAAGCTGCGGGAGCAAGAGCGCGGGGAGGAAG11.3513513513513513No Hit
AAAAAGAAAAGTGGCCGAAAAACAAAAAAAATCCGAAAAAGAATTAAAAA11.3513513513513513No Hit
AAGATAAGTTGACCCATGCACCTCTCCAGATCGGAAGAGCACACGTCTGA11.3513513513513513Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCATCACCTCTCTCTATCTTTCCTCCCCCTTCCTCTCTCTCTCACTTA11.3513513513513513No Hit
GCAGGGAGCGGCAGCCATTGGACGGGGAGGTCGGCAAGCGTGCTTGGAGA11.3513513513513513No Hit
GTGTCTTACTGGGAACTGAGTGATGGTGAGATGGCAGAGATCGGAAGAGC11.3513513513513513No Hit
TTTTTAGTAAGTTTGGATTTGGTCTAAAAGAAACCTGCTTGTTAGACGAT11.3513513513513513No Hit
CGACGGTTTGGGGAGAGAGAGAAAGAGATCGAGATCGAAGAGAGAGAGCT11.3513513513513513No Hit
TCCTTCTTGAACTCGAACTCCTCTAGATAAGCTTCCTTTGATTGATCCGA11.3513513513513513No Hit
CGCCTCTTGCGGGGAGAAGGCGCAGAGGAAAAAAAAAAACCTAGAGCGCA11.3513513513513513No Hit
GGCCAGCAGCCCAGTAGGCAGCAAAGCAAGAGCAAAGGCTGCAGCCTGCA11.3513513513513513No Hit
CCGATGACGCGGCCGCTTCTGTAATCTCCGCGAAGGAAAACACAAGAGAG11.3513513513513513No Hit
ATCCAGCAAAACAGTTATTCTCGGATCTTCTTTGTTGGTCAAGTATACAA11.3513513513513513No Hit
CGAGCCGGAGATTAAAAACCCAAGCGCCCACCCAAAAACCCTAGGGTTTA11.3513513513513513No Hit
GGGTTTTGAAAGAGGAAGAGAGGTGTATGTCAGATCGGAAGAGCACACGT11.3513513513513513No Hit
CACCAATGTATATGTATAGTAGATGAGAAAGGAAATGAAGTGAAGCTTCT11.3513513513513513No Hit
CGTTGCCGGCACTGTTAGATACGGAAGCTACACATTTGTGAATGAGACAC11.3513513513513513No Hit
AGCTCGCAGAGTTACATATGGCAAGAGTTGTATGTTCACATGGTGAGATC11.3513513513513513No Hit
CCGACCTCGAGCTCTTCTCCTTCTTCTTCTCCAGATCGGAAGAGCACACG11.3513513513513513No Hit
CTACCCGTCTCGTGGGCAAAACGGAAGGTTTCCAAGACAAACCTACAAAG11.3513513513513513No Hit
GCAATAAAAAAAAAGAAACTCTATTGCTGGTTTTTTTCTTTGAGGCTGTG11.3513513513513513No Hit
TCATATATCTTTAATATCACTAGAAAAAATACCCGTGCGTTGTAATGGAA11.3513513513513513No Hit
GGTTAGGTAGGTAGACACAACAATGTGAAATTCATCAGATCAAACATGAA11.3513513513513513No Hit
GGAGAAGAAGCCGGGAGGGAGAGAAAGAGAGAAAAGAAGAAAGAGAGAGG11.3513513513513513No Hit
CTGTAAAGATAAAAAAGAATGGTGTCAGGCACGCTACTCAACGATAGAAG11.3513513513513513No Hit
CTCTCCCAAGGCCTCAAGCAACACCTGCACATACAACAACAGATCGGAAG11.3513513513513513No Hit
TTCCGGCGGTCGGCGGACTCGGCGAGGGGGTGGACGGGGTCGACGGCGGT11.3513513513513513No Hit
AGGTTATTTTATCGAAATTTACTTTCTAAATTTTTTGCTTTTAGATTCTT11.3513513513513513No Hit
GTGGGGGAGAGCGCGTGGGGGGAGGCGAGAGGAGAGATCGGAAGAGCACA11.3513513513513513No Hit
GTATCATATTGTAAACATCCAATTTTTTGCCAAAAAACTTAAAATCCTAT11.3513513513513513No Hit
TGTCAAGTACTGAAGTGCACCAGCCAGGCTGCGATAGTCAGTTGGGTCAG11.3513513513513513No Hit
GGTTGGGCGGCGTCGCGCGCGGCCGGAGCGGCGTTGATGTGGGGGCTTGC11.3513513513513513No Hit
CATGTGGTATCTCCTTCATCTATAAAAGGAGGACCTTGCCCTTCAAGCAA11.3513513513513513No Hit
GTTTTTTGTATAGCTGCTGAAAGAACAAAGTTGAGATCGGAAGAGCACAC11.3513513513513513No Hit
TTCTTGGCAGTGGAGAAAGCTATAAGAAGAGGAAGAGGACGACCACAGGG11.3513513513513513No Hit
GTACCTAGGTATCCACCGCAAGCCTTTCCTCTTTTGAACCTAGATCGGAA11.3513513513513513No Hit
CAAGGGGACAAGCCCCCTTTTCTCTCCCTCTTTTCCCCTTTTTCCTCCCG11.3513513513513513No Hit
CATTCTCTTTGTCTGATAGCGGCGGCAAGAATTGACATAGAAAAGCCAGC11.3513513513513513No Hit
TGGTGGTGAGAGTAACGAGGAGCGAGGCGGCGGAGATCGGAAGAGCACAC11.3513513513513513No Hit
AATCCATTTCGTCGCCCAGTAGCTACAACCGTTTTTTTAATCGGTACTGC11.3513513513513513No Hit
GAGATTGGCTGCATGCGGTGGAGAAGAAGTTGGATTTGATCCAGTGTACT11.3513513513513513No Hit
AATTTGTTTTCAAAATAAAATTGTTTTAAAAGCTCAATCCTCTAATTGAC11.3513513513513513No Hit
AAGAAGCAGAAGAAGGAAGGAAGGAGGAAGAAGAGATCGGAAGAGCACAC11.3513513513513513No Hit
TTTGCTTGTGCTGATCTGCTCTGGCTGGCTAAAGGCCATGAGATCGGAAG11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph