FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_NCI3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_NCI3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACAAGGCCGTGGCCAACGTCAAGACGCTGGTGCAGGAAGGCGTGTTCG17.142857142857142No Hit
GCATAGCAGCGACACATGGCCGATGCCCGCGAACGAGATGTGACGCGCGC17.142857142857142No Hit
GGCTTCATCCTTCTGTAGGGCTACCTCCACGACAGCACGATCCCGCCGTT17.142857142857142No Hit
TCTTTGACCACGCTTTTGAATTCGGGCATGTCGCTCATGACAGTGCTCTC17.142857142857142No Hit
TTCCTGTGCCAGACGCACGAGGGCGAGCGATGTCAGCGGCGTGTCTTCCT17.142857142857142No Hit
TCGCAGGCTACATCGACAGCAGCGACGCCACGGGTTGCGTGAACAGCTCG17.142857142857142No Hit
CGTCATCAACTACAACTGCCCTGAGGACGACAAGACCTACGTCCACCGCA17.142857142857142No Hit
GTTCTGCGGGTCGCTGCATTGCGCGCGGTGCGTGCGCGCCTGCACTGGCT17.142857142857142No Hit
ACCCACTCCTATCTAACGGCGTCACCGGCGGGCACCCCGGCCCTCGGCAC17.142857142857142No Hit
GACCGCGTCCGACGTCGCCTTCTCGTTCAAGCGCATCGTCGCCTGTCTCT17.142857142857142No Hit
TGGCTAGACCGTATTCAAACTGGGTCGGTCAGCTGCATCGTTGCCACCGG17.142857142857142No Hit
GCGTCGGGACGCCGCGCTCGCGGGCCGCCTCGCCCACGTTGACCGACGGC17.142857142857142No Hit
GTACAACGCCGACGCCTACCCCGTGTTCCAGCTCGGCTGGTTCCCGGACT17.142857142857142No Hit
ATGTTGCCGGCCGCCGCCTCGGTCACGAGGCACGCGATGTCGTCGCCTGT17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)