FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_NCI2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_NCI2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length251
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGGACCTGAACATCGACGGCGGGTCCCAGCGGTCCGTGCGGCGCGCC12.272727272727273No Hit
GCATAACTTCATCGTGAATCCTACGAGAGTGCGTGTAGATCGAAACCCCA12.272727272727273No Hit
GTACCCCTACCTGCCCCCGCCGTAAGCGATCATGCTGTTGATTTTGCTCG12.272727272727273No Hit
AGGATGTAGGGCGAATGATAGGCAAATCCGTCATTCACATAGCCTGAGAT12.272727272727273No Hit
CTTTCTGGACCATCGAAGGGCGCAACGTGCCCGAGCGCCGCTTCGTGTGG12.272727272727273No Hit
ATACACGTCGATCTCGGGCCCGTGCCTGGACCACGTCGGCCACTGGATGC12.272727272727273No Hit
ATTCAATTGGTTTGGGTGCGTCCAGATCCCCGGATGGTCGATTGGCAGCG12.272727272727273No Hit
AAGCAGCGGTGATGGACGATCAAGGCAATCTGCTCGGCCCGAACCAATCG12.272727272727273No Hit
GATCTCCGCCTCCGACCGGCCCGACATTGCCCTGAACTTGCACGTGATCA12.272727272727273No Hit
CACCGGTCGGGCGTCGCGCGCGCACGGTGGCCCATCCTCGGTGCTGGGCA12.272727272727273No Hit
CCGCTGCTCACCATGCCCGCCAGGCGCGGAACCACCTGGGCAACGTCATC12.272727272727273No Hit
TCTTCTTGCGGTCCAGCTCGTAGTTCATGTGGTCCAGGTTGAACAGGCCG12.272727272727273No Hit
GTTTGCTTCCCTTTCGCCTTACTGTCCGCCGCTCCACCCTTGTTCAGTTA12.272727272727273No Hit
TTGCCGTGTCGACCATCCCGTACTTCTTCATCGGCTATTCGACCGCGTAC12.272727272727273No Hit
CAGGAGATCCACGCGCAGCGGCTTTTTCCTTCAGACAAGGCCGCGGCGAC12.272727272727273No Hit
GTTTTATACAGGGCCGACGGCGATAGCGTCAAGGAAACACAGATGTTTCT12.272727272727273No Hit
CGCACGTTCACCTGGCCCGGCCCCAGGTACACGTAGTCACCCTCACCATC12.272727272727273No Hit
GGTCCGCGCGATTGCGCTGCCGGAGCTGGTTTCGCGCAACCAGTACGCCC12.272727272727273No Hit
CACAAGGAGCGCGCGTGCAACAAGTTTTGAGTAAAACCTTTGGTGGGCTC12.272727272727273No Hit
GTTCATTGGTGTTCCGGCAGACCGCTGCCGAGATTGCCTCCGTTGAACCG12.272727272727273No Hit
CCTCTTCGGAATCGACTGCCGGCGCCTGGCAAGATTCGCCAGCAGCCAAA12.272727272727273No Hit
ATCAAGAACGTTTCCAAGTGGTACGGCCAGTTCCAGGTGCTGACCGACTG12.272727272727273No Hit
GCCGAGATCCACGCGATGGGTCGCGGCACCACCAGCGAGTTCAGCAGGGC12.272727272727273No Hit
GCCAAGGGCAAGGCGTCGACAAGAGCGACGGCCAAGTCTGCCAAGGGCAG12.272727272727273No Hit
GTGAAAATCTCCACCGGTCCGCTGAGCAGCACGGCGCCGGACGGCATTGT12.272727272727273No Hit
TCTCTATTTATTTCCGAGTTCGTCGGCGTCGGCGCGCGGCGGGTTCGATT12.272727272727273No Hit
CGCACGGTCGGCTTCGACTCGCGGCGCGCGCGCAGCACGGCGAGCAGCCC12.272727272727273No Hit
CGATGGCGCTGACCTTCCAGCTCAAGGACGTCTTCGACAGTCCGCGTTCG12.272727272727273No Hit
GGGCTCGGTGGCCAATGTGCAGCAGGCTACTGATGTTAGTCAATTTGCTG12.272727272727273No Hit
CATGGCCTCGTCGACATCGCGAACGTCACCGACTTCGGCGAGACCCAGCT12.272727272727273No Hit
GCAGCATGGTTTGGCGGTTTGGCTCTTCCTGTGCGACCATGCGGCTTTGC12.272727272727273No Hit
GTTGAAGCCGGTGCTGTTCTACGTCGCGATGGTTCGCGAGGGCATCACGC12.272727272727273No Hit
GATCCGCATACCCAGCGGGCTGATCCGGCGGATGATGCCGCACCGCATCA12.272727272727273No Hit
ACTCCCAGCCGGCCTTTCAGGTCGCCCGAGAGGACCTGGGTTCCAACGCT12.272727272727273No Hit
CACGTCCTGCGTGAGGTCCTCGGCGTCGTGCCGGTTGCCGGTCAGGCGGT12.272727272727273No Hit
CCTATCAAGTGCTCGCTGGGCGCAAAAAGGGACGACGTGGAGAAGCCCAT12.272727272727273No Hit
GTGGAGCGCGGCGATACGCTTTCTGCCATCGCCAAGAAGTTCTACGGTGA12.272727272727273No Hit
CACGGGTTCGGGGCGCGTGCCACGTAGTAGGGGGCGGAGCCGGCGGAGCC12.272727272727273No Hit
ATCCCCATTACAGCGACGCCGAACACGCTGAGCAACAATACGACGCCGAA12.272727272727273No Hit
GTCTTGGGGTCCACCTCGGTGCGCAGCACGCCGCTTTCAAGCTGCAACGC12.272727272727273No Hit
CTGGAGCAGAGGCGTAAGCGTGCCCGTATCGAGGTACAGCGTGTGCCCGA12.272727272727273No Hit
TCGTCCGGCTCCGCCTCGTCGGCGTCCTAGTAGACCATTTCGCCGCTCGA12.272727272727273No Hit
CCACGCCGGCCTCGCAAGCCTCTACCCCGGCCGCCAAGCCCTGATCCGCA12.272727272727273No Hit
GTGCCGTGTCGCCGCTCAGCAGCGAGCCCTCCGCGCCGAGCACGACGCAC12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)