FastQCFastQC Report
Mon 22 Aug 2022
000000000-KG266_l01_n02_Tr4_NCI1c_T1_rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_NCI1c_T1_rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACCCCCTGTCAGAACGACCAGGACGCGATTGCGTTTGAAGCTGAAA10.6369426751592357No Hit
CCTCGGGGCCATCGGGGTCATCAGCGTCAACGTCGACGCGACGCACACAT10.6369426751592357No Hit
TCACATACCCATCTTATTACTCCGGGCGATCCTGTTGGCCCGGACCCTCA10.6369426751592357No Hit
ATCCAAGTCATTCAACACCTCCGCCCGCACGGCGGCCGACATCTCGTTTT10.6369426751592357No Hit
GATAGTAAACAAAGCCGCTGCAATCAAATCCAGCTGGTGTATTGCCGCCC10.6369426751592357No Hit
CAGCAATGGATGGTTGGAGAGAAAGTAGCACGAGACGATATTCGCCCTGG10.6369426751592357No Hit
ATTGACGACAGCACCGTGAGCAGGTTGATGCCGCGGTCGAAGTCGGACTC10.6369426751592357No Hit
GTCTTCAATAGTCAGCACCGGCAGGTTGTCTTGATCCGCGCAGCGCACCG10.6369426751592357No Hit
CCATTCCATTCCAATCCATTCCGTTCCTTTCGCTTGCATTCCATTCTATT10.6369426751592357No Hit
GTACTGGAAAGACAAAACGCTTTTGCGATGTTCGACATCGAGAATGGACA10.6369426751592357No Hit
GTCTGGTTCGAGCGCAAGGCCGACCGTGAGGGACGCCTGACGGTGTCCGC10.6369426751592357No Hit
GACTTTTGGCTGAAATGTTGATATTTCAATTCATAATGTGGAGTAGATTT10.6369426751592357No Hit
GGTGAGCAGACCAGCAAGCAAAGCCTGGCCGCCACGACCAGATTTGATGC10.6369426751592357No Hit
AAACTACTATATGATCTATGAACTCGTCTTGCTTTATGGTCGAGATATCA10.6369426751592357No Hit
GATCAGAAGGGATGGCAAAGGAGATTCCAGCTGTGACCTTCATGGTGTTC10.6369426751592357No Hit
CCACAAGGGCGCCAGGCGCCACAGAACCGATCCCGCAGGAATGTTATGAA10.6369426751592357No Hit
GCCCTCCTGCCACTCACCCCCCTCCTCCCCCGCTGTGGCCGCGTTCACCG10.6369426751592357No Hit
GCGAGAAACTGCTCGAGGCCACGGGCGTCCAGCCCGGTGCCACGCACGTG10.6369426751592357No Hit
GTACAGAGGAGTGGGCTCCCTAAGGGTATGAGACCAGCTTCCCTGTTGGG10.6369426751592357No Hit
CGCTATTACTATCTGGTTACCCAGCCGTGGTTTACCCAGCAGTCTGAACG10.6369426751592357No Hit
ACCACGGGGCGGATTTGCGCGTGGTGCAAATGCTGCTTGGGCACAGCGAC10.6369426751592357No Hit
AAGAAAGTCGGCGGAAGATGTATTTCCAGCTCAAAAGGGAATCCGAGCAA10.6369426751592357No Hit
GTCGTGAGCCGGGCGTTCTTGATGACGTCGGCGGCGGCCTCGAGCCACTC10.6369426751592357No Hit
CTTTACACCACCAAAAATGATGAAAGCCGTAAGAATGGTTACGAATACCC10.6369426751592357No Hit
ATATTCGGATGGTATTTTCGACCATAAACTTAAAACTAATAGTATCTATC10.6369426751592357No Hit
CTGTTGACACAACTGTACTCAATTACGCATTTTGTCTCAATTTCGCAGGC10.6369426751592357No Hit
CTGCAAACCGGCTATGCGCATGAAGATGCACCCACGGCAGTGGAGGCCTG10.6369426751592357No Hit
TGTGTTCACCTACGGTCTTTCGAGTCACAGTATTTTTCATCTCCTTCTTC10.6369426751592357No Hit
GCATTTTTGTGAATGATGATGGCAGCCGTCCGGTGCTGGAAGAGTTGAAA10.6369426751592357No Hit
GGTGTCTCTGCTATGGCGAGAAGCTGGACTTCTCCGGAGTATTTGTCTCG10.6369426751592357No Hit
TTCACTGCCGACCCCCAGTTCCGCTCTCCGCCGCTCGCCCCTCCCGCTGG10.6369426751592357No Hit
GCATCCCGGCGCGCGGTGCGAGCGAGCGCAGCAGGCCCTCGTGCCGGGGC10.6369426751592357No Hit
GAATTTCTTCATCTGTCACTTCATCAATTTTCCCGTTTGATTCATCTGCC10.6369426751592357No Hit
GCGCAGTGATTTTAAAGGTTTCATATAAATCAGAAATGCTATGTTCTGCG10.6369426751592357No Hit
TCCGAAGGGGTGTCAAAGGACAACTCTGGGGACAGTTCTGGCACAAGCCA10.6369426751592357No Hit
GATCCAGAGGGGCCACTACGTACCTACCGACCGTCCGGTCAGCGCACCCG10.6369426751592357No Hit
TATATAAAGAACACTTTTTGAAATGACATCAAGGCAGCTAATAATCGGCC10.6369426751592357No Hit
ATACATGATATTGCCAGCCTCCTTTTAGATCATGTTGGTTTATGATATGA10.6369426751592357No Hit
CGGATCGTCCCCATCACGTATTCGTGCTTCCCCGCCCCCACGTCTCTCCT10.6369426751592357No Hit
GCTACTTTCTTCCGCCTTCCTTAGGTGTCGTTCATGTTCCGCTGTGCTCT10.6369426751592357No Hit
AGCATATAAACCGGACTCAGCAAGCATAGATATCGGTACACAAACGTCAG10.6369426751592357No Hit
ATATCGTCGATGTACTGGTCAAGGAAGGCGACATGGTCAAAGCCGGTCAG10.6369426751592357No Hit
TTTATATTCCGTCATTGTTGCTTATTTAGTCATTATAGTCTTTTATTAAT10.6369426751592357No Hit
AAGAAAGAGTTTGTGGAGTGCCTAGTTGACATTGCCCAGCCACGGTAAGA10.6369426751592357No Hit
CCATCTATTAACGCTGAGGCAATGGCGACTGATTCCATAGACGTGGAGCA10.6369426751592357No Hit
CATCATGTCCGCGTTGCGCGATAGGCAGTGCATGTACAGCTGTCTCTTAT10.6369426751592357No Hit
GTACTGCTCCACCTCGTCGCGAGCGTGGACCCCAGCGTGCCGGTGCTGTT10.6369426751592357No Hit
GGATAATATCATGGCGTGTGACATCGCGCTAGAACGGCCGCTGTACGGAG10.6369426751592357No Hit
GATCTGTACTATCGACACAGCTAAAAGTATCAGGGGTTGATGTATTTTCA10.6369426751592357No Hit
ACGCTGGCGCGCCAGCGCGGGTCGGCTGACCAGCAGCAGGAAGGCGATGA10.6369426751592357No Hit
TTTCAGATATAGTGTTCATTCTTTAGCAACCAAGACACGGCCACGCTCTG10.6369426751592357No Hit
AAACTCGCCTCCACATGACAAGCACGCTCGCTTACTGCTTGGCGGGTTCA10.6369426751592357No Hit
ACCGATTCCATCTACACTTCGACCGTCAACCTGACCCCTGCTCCCTCGGC10.6369426751592357No Hit
GGCCAGATCCGAGGCCGATTCAGTATGGCAAATATAGAATAATGGCCGGT10.6369426751592357No Hit
GTCTGCTTTGCACCGTGGACACGTTCTCTCTCACCGCACGTCGGTCCTGT10.6369426751592357No Hit
TCGTCGTGCCGGCCGAGCTCGTCGCGGTGATCGTGCGCGTGAACTGCCCG10.6369426751592357No Hit
TAATACCCCTCTATAGCGATTGTTGTTCCATCTCCCCGCGATCGTAACCA10.6369426751592357No Hit
TCACTCTAGTGCGCGAAAGCGCAGCCAACGCATCATGGACAGCGTCGATC10.6369426751592357No Hit
CTTCGATTCATATTGAATCAAGCCAAATACAGTTGGCCCGCTGCCGCTCA10.6369426751592357No Hit
TGTCACGACTGGTTGCGCGGCGTCCATCCTGATGGACCACGCGATTTTGT10.6369426751592357No Hit
CTGACCGGCATCGTTGTATTCGAATTTCTTCTCTGCTCCGTTAGCATCCT10.6369426751592357No Hit
CGCTCGATGTCGACACGGAGCTCCTCGTCGACATCCTCGTCCCAGCCGAG10.6369426751592357No Hit
ACATTCCCCAATACGACATTTGGGAAGCGTCTTATCGCTTGCTTCAGACG10.6369426751592357No Hit
TGCTGAACGTGGCCGGTGAATTTACGGTGGTTCAAGGCATCGACGGTGAA10.6369426751592357No Hit
GCCTAACCATCCTCGCCCCTCACTTCCCCCTTCCCCCCGTCCTCTTCCCG10.6369426751592357No Hit
CTTCATAGCGGTGACGATGTCGTTCAGTGATGGTCGGCTCGCCGTACAGC10.6369426751592357No Hit
GCCTTCCGCTATGCTTTCGCCGACGTCCCCCCGCATTCCGATCGCGACAA10.6369426751592357No Hit
GTGCCGACGTCGACGACCGCGAGGCCCACGCGGTGCTTGCCTGTCTCTTA10.6369426751592357No Hit
GAACGACAGCGTGACGCCCGCTTCGTAGGCCCATGCATCTAGCACCTTAC10.6369426751592357No Hit
AATTTACGGGACTGTAACACTCGGATTCCGCACCTCCACGACCTTCTACC10.6369426751592357No Hit
GCATACCGTCACGTGGTGATGATCCCGGTGACGTGATTACGATCCGGACG10.6369426751592357No Hit
AGAAACGAAATAAAGATAAAAAGTAGTTTATAAGAAATTAAAAAAAAAAA10.6369426751592357No Hit
GTTGCCAGCGGCGCCCAAGACCCAGTTGTGGAAGCTCGACATCGGGCTAG10.6369426751592357No Hit
GTTCTAGTACGCGTATGGCCTCTCCGCTACAAACCTCATTTCCCTGTCTG10.6369426751592357No Hit
CCGCCATACCGTCTCGGTTTTGGTGTAGGCCTGGGGGTCACCCGTGGCGG10.6369426751592357No Hit
GGCCGGGGGCGCAGGCACTCATCGCTGCCCTGCGCTGATTCGGGAGGCCA10.6369426751592357No Hit
TCGACGACATCTTCGAGACCTTCGGCTGGCGCGTCGTCACGCTCAAGTAC10.6369426751592357No Hit
CGACGGACCGCATCGCCGCCCGGGCCGCTGTCTCGCGTCTGGGTGAGCTG10.6369426751592357No Hit
GATGATGATGAGCGTCATCGGCAGCGCGATCAGCAGGATCGGTGCCACGT10.6369426751592357No Hit
TCTTTTTAACATTTTCATTCATAACAAAATGAAATGGTCAGTCGGGGTTC10.6369426751592357No Hit
GACTGGGACAAGAGCGGCGCGGCTGCCGTGCAGCAGTTCGAGGCGGCGGC10.6369426751592357No Hit
GTATCCGATGGTGATCGCACAGGATCATAAACGGGCATTTGAAGATGACA10.6369426751592357No Hit
ACTCAGCCGTGTCCTACGCCCACCAGCCCATCTTCTTCAGTGGTGGACCG10.6369426751592357No Hit
CATTGCGAACATGACATCGGATACCGACACGATTCCCACCATCCTGCCCC10.6369426751592357No Hit
GCATGCAGGAGCTGCTCTTGCGTGCAGTGTCGTATCGTCGTCATATCAAC10.6369426751592357No Hit
AACCACGGGAGGCGTCGCGCGGGGTGAGCCTGTCTCTTATACACATCTGA10.6369426751592357No Hit
ATATCAGCCGATTTGAACCTTCAGAATTTGGTGATGAGCCGCGTGTCAGT10.6369426751592357No Hit
GGCGGTTTGGTACTTTACTGCTCCCCCCGGACCCCTCAGTTTTTGCTTGG10.6369426751592357No Hit
TATTACTTGCCATCATCTCTTATCGTGAGCCAATCTCAACATCCCGCCTC10.6369426751592357No Hit
ATAATTTCCTTGGCTAGTTCGGGTTTGAGCGTTTGACGGCCCTGCGATTC10.6369426751592357No Hit
ACCTATTTCAATCTCGTATTGTGCATTTTCCACAAAGTTTCTGAAAATAA10.6369426751592357No Hit
CCCACACGTGGTACACGGGTCTCGCCGGCCACCTCTCCTGTCTCTTATAC10.6369426751592357No Hit
TGCCGCAACAGGTGTCGATCCGATCACAGGAGAAGAACTCACCGCAGGCC10.6369426751592357No Hit
GCCCGCGACCGCCCAGATGATCGAGCGGAAGACGCGTCCGCCGTCGAGCG10.6369426751592357No Hit
CCCTTGCCGCCGCCGCCAGCGACCGCCTTGATGAGCACCGGGTAGCCAAC10.6369426751592357No Hit
TAGATTCAGCCCTATTGTCCGCCCACCCTCCTCCCCTCGTTTCGTTCCTT10.6369426751592357No Hit
GCAATACCTAAACGCTGCTTCATCCCAAGAGAAAAACCCTTTACTTTTTT10.6369426751592357No Hit
ATATTCCCCAACCCCCCCAATCTCTTCCCATATACCCCCGTCGCCAACTT10.6369426751592357No Hit
CCTGAATAGGTTAGCACTACTTCAAGATAGCTTCTAGCAGCAGGATCTTG10.6369426751592357No Hit
CATCGGCTGCGCAGAGGACCCAAACCGCGTTCCCTTCCGGTCCGGCGGCA10.6369426751592357No Hit
CCATTATTTATCCATGTTCCAGTAATGCAAGGTGGAACCAACCATACTTA10.6369426751592357No Hit
CCAAGAGCGGCATCGCGCGGGCGCTGGTCTTCGTCTGTCTCTTATACACA10.6369426751592357No Hit
ACTTCTACCTTCTTGACTTTCCGTTCTTCCGCCTCCTCTATTTCGGTCCG10.6369426751592357No Hit
AGCCTGATTCGCGATGACGCGCAGCGCGCCATGCTGCTCGACCTGGACAA10.6369426751592357No Hit
GGCATGGATTCGATCAACGGCAACCAGGCCAGCAACAACGCCACATCTTC10.6369426751592357No Hit
ATTGTATCTGTCTTCCTGCTCAACTGCCGCGTCTGCCCCGCGTCGCCGTC10.6369426751592357No Hit
CTCATACAGATCAGTAAATTCCAGCCGACAATGCAGGTGGCCTCGCCGGC10.6369426751592357No Hit
ATCAATGCCTTGAACCCATGTTGCGCATAAGTATTCATGATGGCATGTGC10.6369426751592357No Hit
GCAGCGACATGGTCACGCAGGAGTCAGGCCCCCGAGGGCGCGCGAGCGGC10.6369426751592357No Hit
GAGCACACTCCTGCTCTTCTTTAGCCATAAAGTGAGTGCCTTGGTCAGAG10.6369426751592357No Hit
CGCCCACGCCGGTCACGCATTCTCCGACGACCGCGCCCAGGCCGCGGCCC10.6369426751592357No Hit
ATTCCAATCAACTCTCTCAGCTAGAGCTGATGATCGCAAATGTCTATCAA10.6369426751592357No Hit
AATCTGTGCAGGGCGCGCACAGATCGCAGCGGTTACGTTGAACGTCCGCC10.6369426751592357No Hit
GCGCAGGCGTTCCTGACCGGGCCGATTTCCCGCTCGCTGGGCGAGCGCCG10.6369426751592357No Hit
AACTTCACCATTCCCTGTTTCTGCTGTTTCCCACGCCCTTTCGACTAAAC10.6369426751592357No Hit
GGTATACTGGTAATCATAGTATATTAAAAGGATGATGTCACATGCTGAAT10.6369426751592357No Hit
TTTCTGCTTTTTATAAACATCCACAATGAACGTTGAACATAATGAGAGTG10.6369426751592357No Hit
TGTGGAGCCTGAGGAAGTATCTGGTGTACCTGTTGGTGGTGCTTTGTCTA10.6369426751592357No Hit
TTCTTAGATTTATCCCCGCTAAAATCGCTAAGAGGAAGCTTTTCCCCCCC10.6369426751592357No Hit
ATCGAAGCCATCTTGGTCCTTATTCTTCTTGTTACTATTTAAATATAATT10.6369426751592357No Hit
CTACGAATACAACGCCAACACTCAGGTGGTGCGCGAGACGCAGCCCGACG10.6369426751592357No Hit
AGACCGACACGACCGACTGCCAGGGCCGTTATTCGTTCTCCGGCCCGTCG10.6369426751592357No Hit
GCGCGCTGCTGCGCGGCCTCGGTCAAGACCCAGTTGCCGATCTCGACAAT10.6369426751592357No Hit
CTATATAACTTTCTTCTTCATGAGAAGATGTAACCAGCCATTCTTCTCCA10.6369426751592357No Hit
TTTCAGCGCATCTACTCGTTCTTTGGCCAACCTGGCAGCTGTAGAGCTAC10.6369426751592357No Hit
CAACAGGCGCTGGCCAACACGGTCAAGCTGGACTCGGTCAATGCCGCCGA10.6369426751592357No Hit
GATGGGCTTTCTCACTGGCAAGGGCGCCAAGGGCGGCAGTATCAGCAGCG10.6369426751592357No Hit
AGGTAATAGCTTGGCGCGAAGCCAACAAACGTGGCTATAGCGATAACGCA10.6369426751592357No Hit
GGCATAGAGAGGAGACACCAAGATGAAATGGTTAGGACAACAAAAACTCA10.6369426751592357No Hit
TTACTGGGGAGGTGGGTTCCCCAATTGTGGCGCGTAGGCAAAGGCTCGTG10.6369426751592357No Hit
TTTCAGAGGAATGTGCTCTTCACTTTCTCTTCCATATCTCCGTAATTGGC10.6369426751592357No Hit
GTTTAGAGATTCTGCCATATAGTGATAATCTGGTCGGTGAATATGTTCGT10.6369426751592357No Hit
TTATAGATTTAGGCGACGTAAATGAAAGTTTCACTATCATTTTTACCTGA10.6369426751592357No Hit
GTACTCGCCCAGGACGACCCCGGCGTACACGGGCTGACTGTCGCACGTCG10.6369426751592357No Hit
GTATCAACTGTGCTAGGAACTGTTTTTGGAGCTTTGGTCTTCGTCTCTGT10.6369426751592357No Hit
CATTCCGCTGCTGCTGAACCGCAAAAAAATCTGGTTGGCCCCCTCAACCT10.6369426751592357No Hit
GGCCATGCCACACACGACCGGGTACACGCTCTGCAGATGCGATGGATTGG10.6369426751592357No Hit
TTATCACTTCTTCCCCTACTCAAGTTGATTTTCTCCCCGTCTCCTTTCGC10.6369426751592357No Hit
CCTTATTGCTTCGGCGCTTGGAACGGGAAATGACCCGTCGCAGATCTCGG10.6369426751592357No Hit
CGGCGGGCGTGGCCCTGCGCATGGACGATCTCTTCACACAACCCAGTGAG10.6369426751592357No Hit
GGATGGCTGTGGCGCTTGCGACGCACTGTGGCAGGAATAGCCCTGTCTCT10.6369426751592357No Hit
GGTCGCGGGCGCTCGAGCGGAACGTGAAGCGCGACTCCTGCCACGCGCTC10.6369426751592357No Hit
CAGGTGGGTGTTGGTCTTGATGGTCTGCTCGACCTGCGAGAAGCTTGCCA10.6369426751592357No Hit
GCCTGCGCCCAATTCCAACAAAAACTCTGTAACGTGTTTGACCAGCGAGC10.6369426751592357No Hit
ACTATCCCCTGATCGAGGTCGGCACGCTGACGCTGACGCGCAATCCCACC10.6369426751592357No Hit
GCAAAATACTTATAATCGTCCATTTTTCCACTCTCACCCCCTATCACTCT10.6369426751592357No Hit
TAGCCGACTGGGTATGGTGGCGCCCTGAGCTTCAGTCGCTGCCACGCCTG10.6369426751592357No Hit
AAATTTACCACGTTGTTTTCGAGCACGTAGGCTTCCATCTCGGCCAGGCG10.6369426751592357No Hit
TTGCGGACCTTGCCGACCTCGATGCCGCGGTAGGTGACCTGCGCACCGGC10.6369426751592357No Hit
TTCTGCACCACCACCACTTTCCCCGTGTTCGCAAGGCCCCACGGTGGCTG10.6369426751592357No Hit
GGCCAACAGATCTGCCCACTGAGTGGTTCGGCGGGGTTGGCCATCGTTTG10.6369426751592357No Hit
TTTCTCTCTTCCCGCGGGTCACCAATCTTTACCTGCTTCTCTGCCTCGGT10.6369426751592357No Hit
CCATATATCCAACAGCTTTGGAATGCTTTAAAAGGACTTTTCACATTTGA10.6369426751592357No Hit
CTGCGCAACTATCTGCTGGCGCGCATCAATGCGCTCACGCAGAGCGGCAA10.6369426751592357No Hit
ATCTGAATCAAGGGGCTGCACTCTTCCTGCACCAGTTCGGCCCGTTTCTC10.6369426751592357No Hit
TTACGGTACTTGTGATCGTAATTGGGCAGCATGTAGTAACCGGGGTCGTA10.6369426751592357No Hit
GAGATGGACCAGACCGGAGGCGTCTTCGCCGAGCAGGTCATCCGCCCCAC10.6369426751592357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)