FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_NCI1b_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_NCI1b_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length251
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCGCAGCGGTTGCAGAAGCCGGAGATGGTAGCGCAAACGCCTCCGCC12.380952380952381No Hit
AACCTGATCGGCAACTGCGTCGCCACCGTGGCCATTGCCCGCTGGGAAAA12.380952380952381No Hit
GCCCACTCCCAAGTGGTGATGAAGCTCTCCACGCGCTGCTGTCTCTTATA12.380952380952381No Hit
GTTTTGGCAGGTGATTCTGCCGGGCACGCTGCTCTCGATTCTGATGGGCG12.380952380952381No Hit
GCGTTGACCGCCGCGTCACCCGTGACCATCCCGACGTTGTCGGCGCCGAA12.380952380952381No Hit
GTGGTCGACCGACTCGGCGCGCGACCGCGACACCTCAGCCACCCGGCGCT12.380952380952381No Hit
ACCTCGCCGACCTGCACCGGCGGGACGTACGAGCGGACGTCGTTGAGCCA12.380952380952381No Hit
ATGACCGACTGGTATTGGCTGCCGGCAGCCGAGTAATCCGGCCACCGCTC12.380952380952381No Hit
CTCCTTTTCTCAAGAACTGAACTTTCGCGGTGTTCACCTCCTAGCAGCCC12.380952380952381No Hit
GCGCCTGGGCGTCGAAACCCTGATCGCCACCGAGTGCGAAATGCAGGACC12.380952380952381No Hit
ACCAACGCCGGGTTCGATAACGGCTCGCACCAGCAATCCGCCGAGGGCCA12.380952380952381No Hit
AGCATTCACAATCGGAGGTGTCGTCCATGGCACCATCACCACCGCTTTCC12.380952380952381No Hit
CTACACTCGCGCCTGTTCTGGGCGACGGCCCACACACACGCCACGATCGC12.380952380952381No Hit
CGCGCACATCGTTCCGCTGCTGGGTGATGTGCCTGCGCTGTCGTTTGCAA12.380952380952381No Hit
AGGTTGACACGATCAACTTCGATCGTCCTGAGAATGCGAAGAACAAGATT12.380952380952381No Hit
CTACTACAGCGTTCCCGACTACTGGCACGCCGGTAAAGAGAAGGCGCTGG12.380952380952381No Hit
ATAACAATAAGAAAAAAGCAGCGATCATCGTGATAAATAAGACTGGGGCA12.380952380952381No Hit
AAACAGGACCGCGGTGCGGAGGCTGACGAAGCGGGTGGACGGTGACCCTT12.380952380952381No Hit
CTCTATGCTAGTAGGCTTTGCTACAGGAATGGATTTACAGGAAATCATTC12.380952380952381No Hit
GGCGAGGTCTCGGTCGTTGACGATCACGTCGTCGGCACGGCTCACGCGGT12.380952380952381No Hit
GAGCATCAGTGGTTCGGTGATTTTCGGCAGCAATGGCGAATTGCGGATAC12.380952380952381No Hit
AGCTCAAGGTGCGCGAAGGGCAATTGGAGGAAGCCTGGATGGAAGCCCTC12.380952380952381No Hit
CCTTGTGGGTCAACCTGAGCATGGTGGCTGTGTGGTTTGCCATCGACAGC12.380952380952381No Hit
CTTTACACATGATTTCATTAGAGCATGTTAACTCATTTACTTGATTGCGC12.380952380952381No Hit
GGTCTGGAGCGCCAGTCATCCGACCGCTTACGCGTCGCGCCGGGCCGGCT12.380952380952381No Hit
ATCACGCCGAGAATCAGCACGACGTGGGTGAGGAAGTCCAGGAATGGACG12.380952380952381No Hit
ATTTATAACGAAGTAGAGACTGATGAAAAACATTTAAAGTGGTATGAAGA12.380952380952381No Hit
AGGTTTAACCAGTTAATGTGCAGAAGGTTTTGCGCGCACCAAAACGGGTC12.380952380952381No Hit
GCGTCGCACAGTTCTTCCGGGTCGACCACTGGCAGGACCCTGTCTCTTAT12.380952380952381No Hit
GCGGAACCCGAGAGGAAATAAGAAAACGTGGAAGGCCCGCATTATACGTG12.380952380952381No Hit
CAGCCAACCTGTTCCATCAAATAGGCACCATGTAAAATGCCGCCTGCATC12.380952380952381No Hit
GGGCAGCCCCGGCATCGACTACGTCGACTGGAAGAAACCGGTTCTGGCTG12.380952380952381No Hit
CCGTAGACCACGGTGTGGCCGACGTTGGGCGTGTACAGGGCGCGTTCGAG12.380952380952381No Hit
CACCCATCGAGCATTTGCTGGCGCTGAAGGACACCCTGGTCAAGGGCGGC12.380952380952381No Hit
GTCCCAGCCACCCGTTGCCGAGACGGCGCAGTCATACTGCTCGGCGGCAA12.380952380952381No Hit
GCGCCGATCACAGTACACAGCACCAGCGCAATAAAAACCGCCGCGTAACC12.380952380952381No Hit
GGATTGAAATCAGTGGTTTTTGGCGAGATGGCAAGCTTTCTGCTTAGACA12.380952380952381No Hit
AGCTAACCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCCTA12.380952380952381No Hit
TTATTCATCTTCCTTTCTCAATCTGGGGAGACTGCGGACAGCCGTGCTGT12.380952380952381No Hit
TCCCTATAGTTATGAGTGGGAGGGCGCCTTGGGGGGGGGGAAGAAGACCC12.380952380952381No Hit
GCAGAGAAAATCTGTGAAGTACTCAGACTTGCCCCATTCAGAGCGGCAGA12.380952380952381No Hit
GCATTGCAGGACGCGACTCTGCCGCCGCTGTTCAATGCCGAGAGCGGCAC12.380952380952381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)