FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C9I3c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C9I3c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAACAACTGGTGCAAACCCGAGGTTGCACCGCCTGTCATGGTGCCCAA12.127659574468085No Hit
GCCTCAACGCGACCTCGGACTCGGGCCGTACCCACACCAACTTCCCGCTG12.127659574468085No Hit
GAGGGACATATCGTGCGAGCTGAAGCGCACCGAGAACACGAGCTCCCGCC12.127659574468085No Hit
CTATGCAAGGCTAGTGCTCGCGGCAGGGGACTAGGGTATTCACTAAGCCA12.127659574468085No Hit
CCTGCCATCATCACTGCGCGCAATGGTGAGAAGGATTACGTTTTCCTCGA12.127659574468085No Hit
GCCCCCGCCACTGCTGGCTTCGCATGCCACCGCCTTCCCGGGCTCCCCCA12.127659574468085No Hit
CTTCTACCCACTCCCCGTCACGAGCTTAAAATCCGCTCCGCCATACTTCG12.127659574468085No Hit
CGATGAAGGCGATCACCGCGTAGGTCAGGCCCGGGAACAGCCACATGCTG12.127659574468085No Hit
CGACCACGTCGACCATTTTGGGCATCGTCACCAGCACGAGAAGCCCCTGC12.127659574468085No Hit
ATTAGTACAGCGCCGCAAACACCTTGCGCCGGTAAGCGGTCACCAGCGGG12.127659574468085No Hit
GAAGTTTCACCAATCCAATTTCTTTCCATTCCCATATAGAAATTGTTATG12.127659574468085No Hit
GGAACGACTACTTTGCAAAATCAGAAGGGTTTAAGCAAAAAGGGACCATC12.127659574468085No Hit
TACCCGAATCCGCCCAGTACCGCCAGGCGTCTCAAGCTAGATTGTCGAAC12.127659574468085No Hit
CGCGCCATCCATGCGCACCATCGGCCTTTCCGCCACCGTCGCCGACCCCA12.127659574468085No Hit
ATGTGGCGTGACACTGACGCCACTGAGGGTGCCGGTGCGCGGCTCCGCCC12.127659574468085No Hit
GCGTTAACGTCGCGCGTACTCAGTCCGATTGATCTGGTCGCACCCAGGAC12.127659574468085No Hit
CTTCATGGAATGGATTGTTGATTTTGTTCGAAATATCATAGGTAGTTCGA12.127659574468085No Hit
AAGAAAACGTCTATCGGATATGGTAAGCAATGCATTCCGGCAGCAGTGGC12.127659574468085No Hit
AGAGATACAACGGGACGATAAGGTCGTCGCAGTTGCTGATCTGTCTCTTA12.127659574468085No Hit
CTCCAATGGTTTCGGTGGACAACCGGTCGCCGTCAACACACCGACCGTGT12.127659574468085No Hit
CACATACACATCCCCGGCACGAGCGATATCATCTGGAAGGGTGATATTCA12.127659574468085No Hit
CACCGACTTCCTTGGTCCAGATGTCGTAGGCCTCGTCATCGCTGGCGTAG12.127659574468085No Hit
TGTGAGATGTAGCCGCTTCTTGCCTTGCCGCGGTTCCCGAATCGCCTCCG12.127659574468085No Hit
CGGTTACGTAAAACGCTTCTGGCTGCACCAGGTCAACCTGAGCATGAACC12.127659574468085No Hit
GTGCGGGACACCCGCAGCCAGTTCCGTCATCCGCGCCGGGTCGTCCCGGC12.127659574468085No Hit
CCTCTCGCCCAGAGCAGCATTCATCTAACGGCACAATTCGCTGTAACGTC12.127659574468085No Hit
CCCTTGAGGCACGCTCAGCCCAGCTTGGCCGGCTGGGCTCTTGCCGCCCG12.127659574468085No Hit
GTCCCGCGACCACCAACAGACCGAGGGCATTGAGCAGGCGACCGCGCAGT12.127659574468085No Hit
ATAAATATGTTCAAAAAAACCACGAGGATCTACTATCTCCGTCTGGCCCC12.127659574468085No Hit
CTATAGGATGGGACAAAGCGGCGTTTGTAGGTGGCTTTCTTTGTTAGAAA12.127659574468085No Hit
ATGCTTTTCCGCTTGAAAAGGCGGCTTATGAAGCGATCCGCCCTGAGATT12.127659574468085No Hit
CTGTTTTACTGATTCTCGCCCACGGCGTCATCGCTGCTGTCCGGGGTGGC12.127659574468085No Hit
GGCCAACGAACCAACAGGTGAACGCCAACCAGAATGTCACCCAGGCACGA12.127659574468085No Hit
ATATGGGCCTTCGCGATTTCGGACAGAAGCTGACAGCCTATCTCGGCAAG12.127659574468085No Hit
GTATTGATCAGTCCTCAGAAATGGTCGAAACCCTAAATCGTGAACCAGCA12.127659574468085No Hit
GGAGTCGAGGCTGTTTTCCAGCAGCTCCTTGATCACCGAGGCAGGCCGTT12.127659574468085No Hit
GCCAGCGTAAAGGGCAGGTACGCTCCTCCTTCATTGTCTATGCGCGGTGC12.127659574468085No Hit
GTACAACAACAAGGAAGCCGTCAAACGTCCGGCCAACTCCCTGTCGCTGC12.127659574468085No Hit
GGCATTGGCCGCCATGCTCAGGCCCGGCAACATGCCAACACCCATTACGG12.127659574468085No Hit
GGCCTGAAACTGCCGTGCATCGAGTTTCGTGGTCGCCGTCTCGATTCGTC12.127659574468085No Hit
CCACGCAAGATCGTGGTGGGGTCTTAGACGGGAAGGCGGCGTCCCCCAGA12.127659574468085No Hit
GGTACTTATCTTCGCCGCTTCTAGCTCATAAGACGGATCTTTCTACCGCC12.127659574468085No Hit
TCGTCGACGTGCGCTTTCCCCCCGCTGACGCCAGGTCCGAACTTATGCAT12.127659574468085No Hit
AGCTACCTTACCTTCGGATCTGCTTCGTCAACGGGGCCGGATGCGAGCCT12.127659574468085No Hit
ACCCTGGTGGTTGCCGTGGTTTGCGACCGCCTGCTGGGCAAGCGCGCCGA12.127659574468085No Hit
TCTCTAGCCTGTGCCCCGCTGTGTTCCTTTCATGTCCATGCCTGAAAGCT12.127659574468085No Hit
CGTAGTCTTTGTTGTGGATTAGGTGGCGTCTTTCACACAATTCACTATCA12.127659574468085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)