FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C8I4c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C8I4c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTTTTCCAGTGGGGGAGGGCTTGCCCCCAGTCGACTCCCCAGCCTCT10.9900990099009901No Hit
GTGACACCGGGCCGGGTCTAACGCTGACTGGAACCGTCCAGGCATCGCCC10.9900990099009901No Hit
TATCCGATGGCTTGGTTGGTATTCGGAGGACTTTTCCCACCTTATTCTGG10.9900990099009901No Hit
GCTACGTCCGGGACGTTTTGACCGTCAAATTACGGTTGACCGCCCAGATG10.9900990099009901No Hit
TTGCTGGCCGCCGCGCTTCTGCGGCTCCAGAAAGCCAATTTCACCATGAG10.9900990099009901No Hit
CGGTTATGGCGACGAGCTGGTGCTGGATCAGATGAACCTGTCGATCCTGC10.9900990099009901No Hit
GCGTAATAATAGGGCAAAATGCCCTATTAAAGCAAGATTTATTTCGTCTG10.9900990099009901No Hit
CACCCAGACGGTGACCAGCATCATGTTGCGCACCTGGTCCATCACCCCGC10.9900990099009901No Hit
TAGCAGACCTATAGTTGGCTCGTCGTAGACGAGCTGGTGGGCAGTCTGCC10.9900990099009901No Hit
CTTTTCATAACGCCGAGGACGGTCCGGAAACGCGCATCGGCACCTGCGGC10.9900990099009901No Hit
GTGTGAAATTTTTTTGGCGGGTTTTTCCAGGCAGTCCGGCTTAGTCAGTA10.9900990099009901No Hit
GGACATCGCCGTGTTCGGGCCTGAGCCCGAGAAACCAAAACCCATCACCG10.9900990099009901No Hit
CACCTAGCTTTAATAGAACCTCCTGCCTACATTCAGGACAGTAAAATTTG10.9900990099009901No Hit
GTCCAATTCCGTCCATTGGTGAAATAGCACCATGAAGCACATGATATAAG10.9900990099009901No Hit
CATATGAAGACAGTCGCTTCATCGTTCCTTCTACTGTATCTGGGTTTGAA10.9900990099009901No Hit
TTAAATCCTTCCCCAGCTCTCGGTGTTTGCGAGTTGTTAAACTTCGTGCA10.9900990099009901No Hit
CCTTGGCCAGGCCACGGTGGGTGACGGGAATCCCGGCGGCTGCCGGGACG10.9900990099009901No Hit
GACGGTCTGACCGGCGGTTACGGGTCTTGGTCGGCAGTATTTGCCCGGGC10.9900990099009901No Hit
AAGAAAGGAAGATACATACATATGTTTGGAATTGGTACAAAAGACCTTGG10.9900990099009901No Hit
CTTCTAGGTTTCTGGGGGGTGGGCTGGCTGGCATTTTCCAGTCTACTGAC10.9900990099009901No Hit
GCCATGGCTCAGCGCGCCCGTGCCAGATCACGTAAAAACTGCAAGGCGTC10.9900990099009901No Hit
TTAGACTTGAGCTGATCTCCTCCAGCCACTCTTTGAGTTGATGAATATCA10.9900990099009901No Hit
AGTCACAACCCGCGAGTACCAGGCTAACCAGGGCTACCGCCGCCGCCAAC10.9900990099009901No Hit
CTTTTTTAGGAGCCCTCATTTCATTAATGATTGCGTTAACAGGTGATGAT10.9900990099009901No Hit
TAACGGGAGAAATTCACCTAACAAATAAAGAGAGGTTCAAGGGAATGAAC10.9900990099009901No Hit
GGCTGATGTGGTGCTGTTCCAGGGCGTCGGTTTCAGCTTCGGCTACCGCG10.9900990099009901No Hit
GTATAAGCGCGAGCGACACGGATTGTCGTCATCACAGCTTCTGTCCCTGA10.9900990099009901No Hit
GCAGTTGACGGATCTGGGTGGCGGACACCGCAAGCGGGGTCTGCCAGACG10.9900990099009901No Hit
TATCAATTGAATGCTGTTTTAAAAGGGCATCAACATCCCGCTTTGCGGAC10.9900990099009901No Hit
ATACATATCTGCCATGACAATCGTAATGCCAGTGATAGAACAAACAATGA10.9900990099009901No Hit
ATGATCTGCTTGCCCACTGCGCGTAACGCCTGCCCGTTGCAATCGAAATC10.9900990099009901No Hit
GGCCTTGGCAAATCTTTGTGCGAGCAGGTCGGCAAACGGTCCTTCACCGC10.9900990099009901No Hit
TTCTTCGACTGGGCACGCAGCGATGGCGTGACCTGGGTGTATATCTTCAA10.9900990099009901No Hit
TATCCAGATAGTGTGCTGACCGTTGATAGTGTACCTGTTTTTGCACGCAC10.9900990099009901No Hit
CATAGCTGACCCTGATCTTGTTGATATTATCGAAAAAGAAGTGTCTGGGA10.9900990099009901No Hit
CAGCGATTGTTTTGTGGGCCGGCGCGGCCGAGGGCTACGATTTCTCCCGG10.9900990099009901No Hit
ACCGGAACTGGTTCAGCGACGCCCTGCAACAGGCCGGGCAGATCACCACC10.9900990099009901No Hit
ATGAAGCACCTTTTCGCAGTGCTGCCACTTCCTCTAGAATGGCACAGCTC10.9900990099009901No Hit
GCTTTATTAACAGAGTCTGCATATGCAAATGAGCATCATAGTGATTTACC10.9900990099009901No Hit
GAACTAAAAGCTGTAGCTGAACAAACGGTGAAAGCTTATGAAGAAGCGAT10.9900990099009901No Hit
ATATTCTGGGACAGGGTCAGTTCGGGTAGGCCCGGGTCAAGGTGCTCAGG10.9900990099009901No Hit
CGCCAAAGGTGTCAAGGTGTGTATCGTCTTTGAAGGCCGCGATGGTGCCG10.9900990099009901No Hit
GCGTAGGACAGGTAGACCATGCGGTCCTGCTTGAAGTCCGGCGACAGCAC10.9900990099009901No Hit
TGATGCAGAACATCCCGATCCTGCTTGTTTCTTTACTCATGTTTTTGCTT10.9900990099009901No Hit
TTTTCATACAGGTTCATCTCCCATCATAAAAAAACAGCAGGAAAATTCCC10.9900990099009901No Hit
AGAATATATTGACTTCCATTTGATTGTTCATGGAAAAACAACCATTGAAG10.9900990099009901No Hit
TGAAGAACCTGGTCGCGATTCTCGAAGAGGCCGGTTATGGCCTTGAAGAC10.9900990099009901No Hit
GAACATGAGAGTGTAAAATATCATGTTCCTGCTTCATTCTGCGTTCAATT10.9900990099009901No Hit
GGCTACAGCTCTCTTGATGTCGGATTCCCTCGATCGGTAAGGCTGGTCGA10.9900990099009901No Hit
ATTAAAGCTTTTTGCGACATTGACAGCATCTTGACCAGTCATCGAGTCAA10.9900990099009901No Hit
GCAGGAAGCCGACACCGACGTTGAACTTTGCGGACTGCAGGACGCCCAGG10.9900990099009901No Hit
CGCGTACTCGCCGCTCAAGGCCAATTGCTGGCCGCTGATGCCGCTGTCTC10.9900990099009901No Hit
CAGTAGCGGGTGTAGCCGTTGACGTAGCCAATCTTGGGTCGGGTGAAGAG10.9900990099009901No Hit
CCGTGTATACCCCGTGCTTTGTGTAATAGGCACCTACGGTCACGGCGCAA10.9900990099009901No Hit
ATTTATTACTCATGATTTAGGCGTCGTGCGGCAAATATGCCAGCGTGTCA10.9900990099009901No Hit
GGCAAGACCACGCTGCTGCGCTGCATCGCCGGGCTGGAGAAGCAGGACAG10.9900990099009901No Hit
ACTGTGCACCACGTCCAGGCTCAGGTTGGCGGCGTCCTTGGGCAAGCCGT10.9900990099009901No Hit
TCTCTACCAAGGGCGAATACTTCTTTTTGATTGCTGCCGCTGATGGTATC10.9900990099009901No Hit
CCCTTATATGTTCTGAATGTGTATTTGCTCAATTAATGAATCAATGTCAT10.9900990099009901No Hit
CACTCATGTCGTCCTGTGCTTCCGGCTCTCCCTGCTCGGCCGAAACGACG10.9900990099009901No Hit
CCTTGGGAGGGCGATAAAGTGGGTACGAGGGCAATGCTACTCTCGATAAG10.9900990099009901No Hit
TACCTATAGATTGCGAACATCCTTTGTGATTTAGCCAGTTATATAATGGA10.9900990099009901No Hit
AGCGAAGAATGTGTTATACCAAAAGTCACTAAGTACGTACTTAGTGACTT10.9900990099009901No Hit
GCACCCCGGCCCCATCGACTCCATTACCGAGCTTCCCCCGAATGATCAGT10.9900990099009901No Hit
GGATATTTTTAGTAAGGCAAAGAAAAGCCCGATGAACATGCCGATGAAAA10.9900990099009901No Hit
CGTTCGGCGGTGCTGAGTCCGCCCCAGATGCCGAAGCGCTCGTCGGCGCT10.9900990099009901No Hit
GAAAACAATGCGCTCATTACTGCTGTGGTTCCTCGGCGTGCCTATCCCTG10.9900990099009901No Hit
GTCTATTTCATCCTCTTCTTGTTCAGTTCGCGCTTCATGTTGCAAAACGG10.9900990099009901No Hit
CCCACAGTGACCGCGTACCGATCTCCTGTAGGAGTGAGCCTGCTCGCGAT10.9900990099009901No Hit
GCAGTGGTACCGCTGCTGGGTGGCCTGGGCGGCGTCAACGGCATGGCGCG10.9900990099009901No Hit
AACTCAGGTCGGCGCTTTCGGCAATGATGCGTTGATAGCGCACCAGGCGC10.9900990099009901No Hit
ATCTGGGTCTCCGCCCACATCTGCCATTGCTTTTCTTAAAGAAGCAAGGT10.9900990099009901No Hit
CAAGCGCTGGGCATCAAAGGCCCTGATGATTTGTGGGGCGGCTACGTCGA10.9900990099009901No Hit
ACCTGATAGTTCAGGATCATACGCGGGGTGAATTTCCTGAACGTCTCGTT10.9900990099009901No Hit
GAAGGAAAGTTTTGGGAAGTCTACCACAATTCAGACCACAGAAACACTAG10.9900990099009901No Hit
GCACATGACATTCGCACAACTACTCAACATGTGTCAGCATCTTCCTTTAG10.9900990099009901No Hit
GGATGAATCATTAGATCTGTTAACGAAAGTGAGAAACGACATTCAAAAGC10.9900990099009901No Hit
ACCTAAAGATATTAGGTGAACAATTTGTATTTGAACGCAACATTTCAGAA10.9900990099009901No Hit
GTGCAGCGTGAAACCGATCGCCTGGGCCTGGAGATGACCGCCAAGCAGAT10.9900990099009901No Hit
GTCTAATCAAAGCACTCACATTCTTTGCTGCTGCAACACAGAAAGAGACA10.9900990099009901No Hit
GACATTCTCTACATTGCCCGCTCGGCGACCACGCAGCGGTTGATCTCGGT10.9900990099009901No Hit
GCGATAGACCGCCGGGGCCTGTTGCAGCAACTGGCGGGGCCGTTCGGCGG10.9900990099009901No Hit
CTTCGCAGCCCAGCGGGAGCAAGCTCCCTCGCCACAGGGTTGCGGATCAG10.9900990099009901No Hit
ACATCTAAATAAATGACCACCTTCTGCTCCTTTCATCTGAAACAAAGAAT10.9900990099009901No Hit
GTCACCGCCGGTTCGGACTTACACCGACCCCGGAGCACGTATGTGTTTTG10.9900990099009901No Hit
CGTGTATCGTCATTCCAAATGATATCGACGGAGAAGCGGTCCGGGCTGAT10.9900990099009901No Hit
TCACCTTGTCTTACCGACTGGAAAAAGCCGACGGCTCCGACGATCTGGAA10.9900990099009901No Hit
GCCCCATACTGCGCCAAAATACCAGAACTGCCCGTCCCAATAAACAGCAC10.9900990099009901No Hit
ACTTATTTTTATTGAGGCGTCTACTATAACTTCGGCGAAAGTCCGTTTTT10.9900990099009901No Hit
GCCTACTAGCACTCTGACGCTTTTAGTCGATGCGGCCTTTTTTCATCTAC10.9900990099009901No Hit
GCTGGGCGGTGCCGGTAACCACCAGCAGGCCGATGGCCAGGCCGGTGAAG10.9900990099009901No Hit
TATGCTAGCTTTGTGCTGCTGACTGTCACAGCGCCATCGTTTGCCCCAAA10.9900990099009901No Hit
AAAATACTGTGACAAATGTATTGTCTTTGCGGATCTGATTCAAAAATTGG10.9900990099009901No Hit
CTTCTATATTGGCCTTGAGTTCATCGTTGGACGGCTTGATCCGTACATCC10.9900990099009901No Hit
TGAAAAAAGTGTCAATGATTACCACTCCTATTGTCCGCCGACCTCGCCAT10.9900990099009901No Hit
GTGCACGTTGATGCCGAAGCGCTGGATCCACACGTGCTTGAATTCAAGCG10.9900990099009901No Hit
TTATGAAACACGCCAAATGAAAATCGTGAATGACGAAGATGTTCTAAACC10.9900990099009901No Hit
GCTCGGTGAGCCGGTCAACCCCAACGATCACGTCAACTGCGGCCAGAGCA10.9900990099009901No Hit
GTCGGTGAGGTGGATGCCTTTAGCCGACTTCTGGCTCGGCTCCAGGCGTG10.9900990099009901No Hit
GACTGGGTCTGGGAATCCCCATTTATCACGAATTACTTGGTGCGTTCGTT10.9900990099009901No Hit
ATCTGAAGAAGGTCGGGCGCATCGGCCTCAAATCGGTGATCTACTTTGAA10.9900990099009901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)