FastQCFastQC Report
Sun 21 Aug 2022
000000000-KG266_l01_n02_Tr4_C8I2c_T1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-KG266_l01_n02_Tr4_C8I2c_T1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTTGTAGCGAGGCTGGGTCGCTTTGCCCTCAGGAAACAAGGTCCTGGA11.2345679012345678No Hit
TTCCTGGTGAGGCCGAATCACGCCGCAACCTTCGCATGCAATGTCCACCA11.2345679012345678No Hit
GTTTTTCCACCTTGTCCGGGGTCGGCGGGCAGCTCTGGCGGAAGGTCAGG11.2345679012345678No Hit
AATCAAAAGATCGCAGTCCTCGCCAGCTTCTACAAGGGCCGGTAGCTAAA11.2345679012345678No Hit
CGCATGCCGTCCTTGCCGACCAAGGCTTTCACTCCTGTTCGTCCGTCTCG11.2345679012345678No Hit
CCTCTAATGTATTAATTATTGCCGCCTGTGCCGTCGCGGAACGCAGCGAC11.2345679012345678No Hit
CGCAACAGAGTCCCTGAGCATGATGGACCACAATCCGACTGCACCTTCAC11.2345679012345678No Hit
GAACTGGCCCTGCTTGGAGCGAAATTCGGCCAGCTCCTGGGTTTTCTGGC11.2345679012345678No Hit
GCCTTGAATTCCAGCAATACTTTGACCGCTTCGGCACGGCCGTGGTACGC11.2345679012345678No Hit
AGTATACGGCCAGGAGGACTCGGACAACGACCACCGGTTGGATCAGGACC11.2345679012345678No Hit
CCCAAAAACGGTTTCGCTTGATGGTTTTTGAGTAAAACGTGCAATGATAG11.2345679012345678No Hit
CTTTGGCGTTTTTTAAGGCTGGAACCCATGTCGGATGAACAAATGAGGTG11.2345679012345678No Hit
GAGGTAATCTTCGTGTCCATCGAAAAGGCCTGCGAGACTAGACATTCCTG11.2345679012345678No Hit
CTATGACATTCAGCCAGCCTGCGGCACGGGTCTGGAAGCGGCGCTGCTGG11.2345679012345678No Hit
GTACTGATCGACAACAGCAAGGTGGTCTTCGATGGCGCGCAGCGTTATGA11.2345679012345678No Hit
GAATCGGCCACGATGATGTCGCCGGGCTGCACGGTGGCTCCGTCGCATGC11.2345679012345678No Hit
ATATACAATGGAACATCAGCCCACGGAAAACTTCCTTCTAATGCAAGCCC11.2345679012345678No Hit
TTGTAAGCACCCGGCAATGCTAAATGATTTTTGAGCATTTCATAAGATTT11.2345679012345678No Hit
ATCACAACACTTCGACTGGCTAGTGTTACTGGCCCCTTAACGTGCTTCGG11.2345679012345678No Hit
GATCAAGAATGTCTTCATTGATCGCCCGTGGCGTGCCGACGCTGCTTTGG11.2345679012345678No Hit
ATGTCCTGCAGCATTACCCCACGCAGGCGTGCACACCCAAAGATGACTGC11.2345679012345678No Hit
GTACGCGCGGATCATCGTCGAAGAAGCCCATCGGCGCGGCATCGATGTGC11.2345679012345678No Hit
CCCCGGGCCAGGCGAGGAATCCACCGTCGCGCAGATCGATGATCTGCCAC11.2345679012345678No Hit
GTTCAATGAAACCCTGCAATTGCACTGACCTGTTTCACCATCACCAATGC11.2345679012345678No Hit
TTTCCGTACCGAGCAAGGCCGTATCTACCAGCGTCCGTTCGGCGTCCAGT11.2345679012345678No Hit
GTCTATAACCGCGAATTCGGCATGCCGGGTGGCGAACCGGTCGGTCTTTT11.2345679012345678No Hit
GGGCAGTGGCATAGAGAGCTATACACGGCCGCAGGTGCACAACTCTATGA11.2345679012345678No Hit
GTAAAAGACGACACATTTTGTTGCATCCAAATATCGGTTGGATCCAACGT11.2345679012345678No Hit
CCGTCAGCCTTGCGGCTCCCGGGCCCCCTTTTCCCCCCGGCAACTTCAGC11.2345679012345678No Hit
GTGTATAGCGCCGGACACCGCACTCCTAACCGCCTTTCTCTTGCAGTGTA11.2345679012345678No Hit
ATACCACTGCCTTCGTCTTCGACGATCAGATCGACCGTGTGTTCGCCGGC11.2345679012345678No Hit
CCCCTAATGATGGTAAGTTGGTGATATGGGAATCGAGGAAGGTGAAGTGA11.2345679012345678No Hit
ATATCTGGTCGCGGTGCATGGCATAGAGACGACGGAGGAGGCGACGCAGG11.2345679012345678No Hit
CTCTATGATAATTTCGATTTGTCACTTCACTACGGGAAACTAGAAGATCA11.2345679012345678No Hit
GATCGCCACGGTTACGTGTTGCTGGCCTGCAGTGACGCGGATAACCCGGC11.2345679012345678No Hit
CCTCAGAGATCTCGATACTCTCAATACCCGGGTGTTCCGCCCTCACGGCA11.2345679012345678No Hit
GGGGCAGTACCCCCAAAGGGATCAGTACCCGTCGGACGACGAGATGCGCC11.2345679012345678No Hit
CCGCTGGCCCTTCTGCGGTTCCCGCTGGCTGCCTTGCTCCTTTTCCGCAC11.2345679012345678No Hit
CTTCGCCCCCATCTCGACGCGCCCCCCGCCCTGCCCCCCCCACCCGCCGG11.2345679012345678No Hit
GCGCGTCATCTCCCACAAGCCGGCGGTGACGTTTCAAGTTCCCATCTCGG11.2345679012345678No Hit
CTTTGGTTGGTTTGGGTGAATATCCGTTTTTTGCGGTGTTGCGGATGGCG11.2345679012345678No Hit
CATCGACCTCCATCAGCGGATGCACGGCCCTCCGCACCCCTCCCCCACCC11.2345679012345678No Hit
AAACAAGTCGACACAGTGCCGAGCCACCGGGCCGTCTCACTTGTCGGCAC11.2345679012345678No Hit
GGTGAAAGCGTCACTGGATCATCTATAACTAGCGACTCTTCCCACAGACC11.2345679012345678No Hit
GTCTCATGGTCGTGGCGCTGGCCGGCAGCCTGAGCGGCGAATTGCCGGGC11.2345679012345678No Hit
GGGTAAGAGATGAAATTGGAGATCGATTGAAACAGTTTGCTGAGACTGGA11.2345679012345678No Hit
GTGCCTGATTTGCACACACGAAAGGATGACTATTTGAGAGAGACATTAGC11.2345679012345678No Hit
TTGGTGACGATTCGCGGATCTTTTTGAAATGTTTTGATAGCGTTCTCATA11.2345679012345678No Hit
TGCTCGTATCGCACAACCTCGCGGTGGTGCAGCACGTGGCCGGGGATGTG11.2345679012345678No Hit
CCGCCATGCAACCGGCGCCGGCCGAGGTGATGGCCTGGCGGTACACGTGG11.2345679012345678No Hit
CCGACGAATTGCGAACCCATGTTCAGCTTGCGCAGACGACCGACCGGGAC11.2345679012345678No Hit
GTCAGTGACCGCGCCGGCCAATGCCAGGAGATGAAAGTGGCTGGTGGGCA11.2345679012345678No Hit
ATATCGACCAACGCACCGACCGGTACGAGCGTCAGGGCGGCCATGCCTGT11.2345679012345678No Hit
CTTCTAGGCGGCGCGGCGTCTGCAGCCGGTGCAGCCCCGGACGGGACATC11.2345679012345678No Hit
GACTCGTTCTCGGGCCCGTCGAACCTGAATTTCATCGCTGCTGTTACTGT11.2345679012345678No Hit
AAAGATAGGTGATCCTGGTGGGGCGGGCTGTGTCGAGGTGTCCCTGCTTG11.2345679012345678No Hit
ACAGCGATGTTGACGAAAAGTGAGCATTGGTCCTACGAACGCGAATGGCG11.2345679012345678No Hit
ACCGACTTCACGCCCGGATGGTTGGTGCGCTTGCCCAGATTTTCGTAGGC11.2345679012345678No Hit
GCTGAAAGCGGAGAAGGCCTATCGGGCGGAACTCGCCAAAACCACCATCG11.2345679012345678No Hit
GTCTGTATCCTTTTCACGTTTCCTACGCCCCTAGCGGCCATTGGCGACCA11.2345679012345678No Hit
CTTTTTCAATGGTGTTTCGCACAAAACGGCCGTTGCTGAATTTAGCAGGA11.2345679012345678No Hit
GGCACAAGCAGGTAGGGGGATCCAACCCAGTTGAACGAGACTCTTCTATC11.2345679012345678No Hit
GAGAAAATCCTGTTCATTGTTCGACTTCCACTATGAGTGCTCTTTCAAAG11.2345679012345678No Hit
GGACCACCTGGTTCCATCCTCCCGTGCCGGCGCAACCCGTGGGACTGGCG11.2345679012345678No Hit
CTCGAGGCATTGTCGACGTGTCGTCCCGTTTCTGCGTTTTCAGCGAGAAG11.2345679012345678No Hit
ACATACTGCTTACCGGCGCACTGGGGCCGATGGTGCCTCTCTCGCCGGGA11.2345679012345678No Hit
CTTGATATAAGCGCCGATATCCGTCTGGGTCTTCTCATGGGTGGAGTAAT11.2345679012345678No Hit
AGATCCACTTGCTGTCGGTAATCGTCAGGATCGCCGCAGACAGAAGCTGC11.2345679012345678No Hit
ACGTAAATGAGGTGTTTGTTAAAGGCAATAACCGCATGACAAATTGGTTT11.2345679012345678No Hit
GCCCAGCACGTATTGCCCTCCAGCGTACCGACATTGCGCGGTGGCAGCTT11.2345679012345678No Hit
CGTATCCGGTGGATGGCACGATTCGCCGTGAAGTGTTCAGCCGGCGCGGT11.2345679012345678No Hit
CTACAAACGCATATCCCTTATGACTCGCCGAGGCTCCGCTCCCTGGGTGA11.2345679012345678No Hit
CTCCACAGTTATAGTGGCCACCAGTGGCGCACCCAGTCCCGCCAGACCTC11.2345679012345678No Hit
GAGCGAGCCTGAAGAGAAAATGCCGACTGTGAACACTGAAAATTGCAAGC11.2345679012345678No Hit
AGATCCCCCGGGTCCACGGATGGCTGTACGCGGCGTATTCGATCTTCAGC11.2345679012345678No Hit
GCTTTTCCATTCTCATCCATGACGATTAAGGAATGCATCGCCGTACTAAT11.2345679012345678No Hit
TTTGTATGGAGCAAACCCGCAAGCGCCGGATTCGCTCCGTTTGATTAAAA11.2345679012345678No Hit
GTTTCTGTTTCTTCATTGGGCGTTTTGCTGCTGCTCATTCTCTTTTCACG11.2345679012345678No Hit
AGCCCGGGGCGCCGGTATGGCGGACGGGTAAGCCGGACGCGTTCCCCGCT11.2345679012345678No Hit
GGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCCATGCCGCGTGTG11.2345679012345678No Hit
CCTCAATCCCACTCGCCGCTGATTCCTTTAACGCCTGCTCGTTTTCCTGA11.2345679012345678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)